{"id":{"repo_id":"essex","oai_identifier":"oai:repository.essex.ac.uk:17521"},"canonical_url":"https://search.dev.ndltd.org/etd/essex/oai:repository.essex.ac.uk:17521","repository":{"repo_id":"essex","name":"University of Essex","base_url":"https://repository.essex.ac.uk/cgi/oai2"},"display":{"title":"The role of soluble factors affecting the major histocompatibility complex class I molecules In an IN VITRO model of the fetomaternal interface","abstract":"Human main histocompatibility complex is encoded in the short arm of chromosome 6 and can be divided into three main regions based on the proteins that it encodes. We have class I, class III and class II. Class I encodes for proteins that are expressed on the cell surface of almost all somatic cells and is related to the presentation of self-antigens. Class I can be sub-divided into two groups known as classical class I (Ia) and non-classical class I (Ib), and expressed on the cell surface as human leukocyte antigen (HLA). HLA-G is one of the members of HLA-Ib and, together with HLA-E and HLA-F, is thought to play a key role in maternal tolerance to the semi-allogenic embryo, one haplotype comes from the mother, and is shared with her, while the other comes from the father. At the trophoblast stage, embryos do not express HLA-II and only express HLA-C (Ia member). Based on these facts, important questions about the role of these antigens during pregnancy have arisen. Our premise is that PreImplantation Factor (PIF), a 15 amino acid peptide secreted only by viable embryos, seems to plays a key role in this regulation. In this study we have used the JEG-3 cell line as a human trophoblastic model to study the effect of PIF on HLA-I expression. JEG-3 cells were incubated at different concentrations and time points of PIF. Using a wide variety of techniques, we could detect that PIF significantly induced HLA-I expression, mainly HLA-G and -E, increased their invasion, proliferation in vitro. Also, PIF modified protein profile, detected by 2D electrophoresis. Compared with the untreated cells, 14 proteins were over-expressed and 8 were under-expressed. Our study suggests that PIF, has a regulatory effect on HLA-I in this cellular model and the fact that not only HLA-G was over-expressed can suggest new regulations pathways under the control of HLA-E.","abstract_html":"Human main histocompatibility complex is encoded in the short arm of chromosome 6 and can be divided into three main regions based on the proteins that it encodes. We have class I, class III and class II. Class I encodes for proteins that are expressed on the cell surface of almost all somatic cells and is related to the presentation of self-antigens. Class I can be sub-divided into two groups known as classical class I (Ia) and non-classical class I (Ib), and expressed on the cell surface as human leukocyte antigen (HLA). HLA-G is one of the members of HLA-Ib and, together with HLA-E and HLA-F, is thought to play a key role in maternal tolerance to the semi-allogenic embryo, one haplotype comes from the mother, and is shared with her, while the other comes from the father. At the trophoblast stage, embryos do not express HLA-II and only express HLA-C (Ia member). Based on these facts, important questions about the role of these antigens during pregnancy have arisen. Our premise is that PreImplantation Factor (PIF), a 15 amino acid peptide secreted only by viable embryos, seems to plays a key role in this regulation. In this study we have used the JEG-3 cell line as a human trophoblastic model to study the effect of PIF on HLA-I expression. JEG-3 cells were incubated at different concentrations and time points of PIF. Using a wide variety of techniques, we could detect that PIF significantly induced HLA-I expression, mainly HLA-G and -E, increased their invasion, proliferation in vitro. Also, PIF modified protein profile, detected by 2D electrophoresis. Compared with the untreated cells, 14 proteins were over-expressed and 8 were under-expressed. Our study suggests that PIF, has a regulatory effect on HLA-I in this cellular model and the fact that not only HLA-G was over-expressed can suggest new regulations pathways under the control of HLA-E.","abstract_has_math":false,"creators":["Hakam, Soukaina Miya"],"institution":"University of Essex","degree_name":"phd","degree_level":"doctoral","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2016,"date_issued":"2016-06","date_published":"2016-06","updated_at":"2026-07-24T02:18:09Z","subjects":["QH301 Biology"],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":null,"outbound_label":null,"outbound_source":null},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Hakam, Soukaina Miya"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2016-06"]},{"key":"dc:date.issued","label":"Date","values":["2016-06"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["School of Biological Sciences"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["University of Essex"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://repository.essex.ac.uk/17521/"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["doctoral"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["phd"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["QH301 Biology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://repository.essex.ac.uk/17521/3/The%20role%20of%20soluble%20factors%20affecting%20the%20major%20histocompatibility%20comlex%20class%20I%20molecules%20in%20an%20in%20vitro%20model%20of%20the%20fetomaternal%20interface%20Hakam%20thesis.pdf"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Human main histocompatibility complex is encoded in the short arm of chromosome 6 and can be divided into three main regions based on the proteins that it encodes. We have class I, class III and class II. Class I encodes for proteins that are expressed on the cell surface of almost all somatic cells and is related to the presentation of self-antigens. Class I can be sub-divided into two groups known as classical class I (Ia) and non-classical class I (Ib), and expressed on the cell surface as human leukocyte antigen (HLA). HLA-G is one of the members of HLA-Ib and, together with HLA-E and HLA-F, is thought to play a key role in maternal tolerance to the semi-allogenic embryo, one haplotype comes from the mother, and is shared with her, while the other comes from the father. At the trophoblast stage, embryos do not express HLA-II and only express HLA-C (Ia member). Based on these facts, important questions about the role of these antigens during pregnancy have arisen. Our premise is that PreImplantation Factor (PIF), a 15 amino acid peptide secreted only by viable embryos, seems to plays a key role in this regulation. In this study we have used the JEG-3 cell line as a human trophoblastic model to study the effect of PIF on HLA-I expression. JEG-3 cells were incubated at different concentrations and time points of PIF. Using a wide variety of techniques, we could detect that PIF significantly induced HLA-I expression, mainly HLA-G and -E, increased their invasion, proliferation in vitro. Also, PIF modified protein profile, detected by 2D electrophoresis. Compared with the untreated cells, 14 proteins were over-expressed and 8 were under-expressed. Our study suggests that PIF, has a regulatory effect on HLA-I in this cellular model and the fact that not only HLA-G was over-expressed can suggest new regulations pathways under the control of HLA-E."]},{"key":"dc:format","label":"Dc Format","values":["text"]},{"key":"dc:title","label":"Title","values":["The role of soluble factors affecting the major histocompatibility complex class I molecules In an IN VITRO model of the fetomaternal interface"]}]}],"canonical_facts":{"dc:creator":["Hakam, Soukaina Miya"],"dc:date":["2016-06"],"dc:date.issued":["2016-06"],"dc:description.abstract":["Human main histocompatibility complex is encoded in the short arm of chromosome 6 and can be divided into three main regions based on the proteins that it encodes. We have class I, class III and class II. Class I encodes for proteins that are expressed on the cell surface of almost all somatic cells and is related to the presentation of self-antigens. Class I can be sub-divided into two groups known as classical class I (Ia) and non-classical class I (Ib), and expressed on the cell surface as human leukocyte antigen (HLA). HLA-G is one of the members of HLA-Ib and, together with HLA-E and HLA-F, is thought to play a key role in maternal tolerance to the semi-allogenic embryo, one haplotype comes from the mother, and is shared with her, while the other comes from the father. At the trophoblast stage, embryos do not express HLA-II and only express HLA-C (Ia member). Based on these facts, important questions about the role of these antigens during pregnancy have arisen. Our premise is that PreImplantation Factor (PIF), a 15 amino acid peptide secreted only by viable embryos, seems to plays a key role in this regulation. In this study we have used the JEG-3 cell line as a human trophoblastic model to study the effect of PIF on HLA-I expression. JEG-3 cells were incubated at different concentrations and time points of PIF. Using a wide variety of techniques, we could detect that PIF significantly induced HLA-I expression, mainly HLA-G and -E, increased their invasion, proliferation in vitro. Also, PIF modified protein profile, detected by 2D electrophoresis. Compared with the untreated cells, 14 proteins were over-expressed and 8 were under-expressed. Our study suggests that PIF, has a regulatory effect on HLA-I in this cellular model and the fact that not only HLA-G was over-expressed can suggest new regulations pathways under the control of HLA-E."],"dc:format":["text"],"dc:identifier.uri":["https://repository.essex.ac.uk/17521/3/The%20role%20of%20soluble%20factors%20affecting%20the%20major%20histocompatibility%20comlex%20class%20I%20molecules%20in%20an%20in%20vitro%20model%20of%20the%20fetomaternal%20interface%20Hakam%20thesis.pdf"],"dc:language":["en"],"dc:publisher.department":["School of Biological Sciences"],"dc:publisher.institution":["University of Essex"],"dc:relation.isreferencedby":["https://repository.essex.ac.uk/17521/"],"dc:subject":["QH301 Biology"],"dc:title":["The role of soluble factors affecting the major histocompatibility complex class I molecules In an IN VITRO model of the fetomaternal interface"],"dc:type":["Thesis"],"dc:type.qualificationlevel":["doctoral"],"dc:type.qualificationname":["phd"]},"updated_at":"2026-07-24T02:18:09Z"}