{"id":{"repo_id":"emich","oai_identifier":"oai:commons.emich.edu:theses-1796"},"canonical_url":"https://search.dev.ndltd.org/etd/emich/oai:commons.emich.edu:theses-1796","repository":{"repo_id":"emich","name":"Eastern Michigan University","base_url":"https://commons.emich.edu/do/oai/"},"display":{"title":"Association of glpQ with serum-resistance in salmonella typhimurium","abstract":"

Salmonella typhimurium is a leading cause of infectious human gastroenteritis and systemic disease when it escapes into the bloodstream. The human serum complement system is central in fighting systemic bacterial infections. S. typhimurium exhibits resistance to killing by complement (serum-resistance), a trait conferred by several known virulence genes. This study investigates a potential chromosomal complement-resistance gene in S. typhimurium tagged by TnphoA insertional transposon mutagenesis. S. typhimurium strain EM876 has a chromosomal TnphoA insertion and reduced serum-resistance. Inverse PCR and subsequent DNA sequence analysis reveal the insertion to be in the gene glpQ. glpQ codes for a periplasmic glycerophosphodiester phosphodiesterase involved in glycerol degradation. Complementation assays using a clone of glpQ on a multicopy plasmid are consistent with a role for glpQ in serum-resistance. glpQ is preferentially expressed at 37°C versus 30°C. This is similar to traT and suggests that multiple temperature-regulated genes may contribute to serum-resistance in S. typhimurium.

","abstract_html":"<p><em>Salmonella typhimurium</em> is a leading cause of infectious human gastroenteritis and systemic disease when it escapes into the bloodstream. The human serum complement system is central in fighting systemic bacterial infections. <em>S. typhimurium</em> exhibits resistance to killing by complement (serum-resistance), a trait conferred by several known virulence genes. This study investigates a potential chromosomal complement-resistance gene in <em>S. typhimurium</em> tagged by Tn<em>phoA</em> insertional transposon mutagenesis. <em>S. typhimurium</em> strain EM876 has a chromosomal Tn<em>phoA</em> insertion and reduced serum-resistance. Inverse PCR and subsequent DNA sequence analysis reveal the insertion to be in the gene <em>glpQ</em>. <em>glpQ</em> codes for a periplasmic glycerophosphodiester phosphodiesterase involved in glycerol degradation. Complementation assays using a clone of <em>glpQ</em> on a multicopy plasmid are consistent with a role for <em>glpQ</em> in serum-resistance. <em>glpQ</em> is preferentially expressed at 37°C versus 30°C. This is similar to <em>traT</em> and suggests that multiple temperature-regulated genes may contribute to serum-resistance in <em>S. typhimurium</em>.</p>","abstract_has_math":false,"creators":["Mitchell, Kristie Carlotta"],"institution":null,"degree_name":"Master of Science (MS)","degree_level":"Open Access Thesis","degree_discipline":"Biology","degree_department":null,"school":null,"contributors":["James L. VandenBosch, Phd, Chair","Daniel Clemans, Phd","David Kass, Phd"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-11-15T08:00:00Z","date_published":"2011-11-15T08:00:00Z","updated_at":"2026-07-24T02:16:51Z","subjects":["salmonella","gastroenteristis","serum","qlpQ","Biology"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://commons.emich.edu/theses/427","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["James L. VandenBosch, Phd, Chair","Daniel Clemans, Phd","David Kass, Phd"]},{"key":"dc:creator","label":"Author","values":["Mitchell, Kristie Carlotta"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2012-09-24T07:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Open Access Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science (MS)"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["salmonella","gastroenteristis","serum","qlpQ","Biology"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://commons.emich.edu/theses/427"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["

Salmonella typhimurium is a leading cause of infectious human gastroenteritis and systemic disease when it escapes into the bloodstream. The human serum complement system is central in fighting systemic bacterial infections. S. typhimurium exhibits resistance to killing by complement (serum-resistance), a trait conferred by several known virulence genes. This study investigates a potential chromosomal complement-resistance gene in S. typhimurium tagged by TnphoA insertional transposon mutagenesis. S. typhimurium strain EM876 has a chromosomal TnphoA insertion and reduced serum-resistance. Inverse PCR and subsequent DNA sequence analysis reveal the insertion to be in the gene glpQ. glpQ codes for a periplasmic glycerophosphodiester phosphodiesterase involved in glycerol degradation. Complementation assays using a clone of glpQ on a multicopy plasmid are consistent with a role for glpQ in serum-resistance. glpQ is preferentially expressed at 37°C versus 30°C. This is similar to traT and suggests that multiple temperature-regulated genes may contribute to serum-resistance in S. typhimurium.

"]},{"key":"dc:title","label":"Title","values":["Association of glpQ with serum-resistance in salmonella typhimurium"]}]}],"canonical_facts":{"dc:contributor":["James L. VandenBosch, Phd, Chair","Daniel Clemans, Phd","David Kass, Phd"],"dc:creator":["Mitchell, Kristie Carlotta"],"dc:date.available":["2012-09-24T07:00:00Z"],"dc:description.abstract":["

Salmonella typhimurium is a leading cause of infectious human gastroenteritis and systemic disease when it escapes into the bloodstream. The human serum complement system is central in fighting systemic bacterial infections. S. typhimurium exhibits resistance to killing by complement (serum-resistance), a trait conferred by several known virulence genes. This study investigates a potential chromosomal complement-resistance gene in S. typhimurium tagged by TnphoA insertional transposon mutagenesis. S. typhimurium strain EM876 has a chromosomal TnphoA insertion and reduced serum-resistance. Inverse PCR and subsequent DNA sequence analysis reveal the insertion to be in the gene glpQ. glpQ codes for a periplasmic glycerophosphodiester phosphodiesterase involved in glycerol degradation. Complementation assays using a clone of glpQ on a multicopy plasmid are consistent with a role for glpQ in serum-resistance. glpQ is preferentially expressed at 37°C versus 30°C. This is similar to traT and suggests that multiple temperature-regulated genes may contribute to serum-resistance in S. typhimurium.

"],"dc:identifier":["https://commons.emich.edu/theses/427"],"dc:subject":["salmonella","gastroenteristis","serum","qlpQ","Biology"],"dc:title":["Association of glpQ with serum-resistance in salmonella typhimurium"],"thesis:degree_discipline":["Biology"],"thesis:degree_level":["Open Access Thesis"],"thesis:degree_name":["Master of Science (MS)"]},"updated_at":"2026-07-24T02:16:51Z"}