In this project, we studied the effect of silver and gold nanoparticles of various sizes on the fluorescence properties of tris (2, 2-bipyridyl) dichloro ruthenium (II) hexahydrate (Ru (bpy)3 2+), tryptophan and phenylalanine. For (Ru (bpy)3 2+) absorbed on silver and gold nanoparticles, the fluorescence intensity was quenched and showed a significant increase in the fluorescence lifetimes. These quenched intensities were due to energy transfer processes from the donor (Ru (bpy)3 2+) to the metal nanoparticles. Conversely, tryptophan showed enhanced fluorescence intensities with an increase in the size of the nanoparticles, which may be attributed to the effect of fluorescence resonance energy transfer and the overlap of the emission band of fluorophore with surface plasmon resonance bands of metallic nanoparticles. Phenylalanine, however, showed a decrease in fluorescence intensities in the presence of gold and silver nanoparticles. Thus the observed fluorescence properties of tris (2,2-bipyridyl) dichloro ruthenium (II) hexahydrate, tryptophan, and phenylalanine on silver and gold nanoparticles may be useful for the design of new analytical tools that can be used in the fields of labeling, biosensing, and bioimaging.
","abstract_html":"<p>In this project, we studied the effect of silver and gold nanoparticles of various sizes on the fluorescence properties of tris (2, 2-bipyridyl) dichloro ruthenium (II) hexahydrate (Ru (bpy)3 2+), tryptophan and phenylalanine. For (Ru (bpy)3 2+) absorbed on silver and gold nanoparticles, the fluorescence intensity was quenched and showed a significant increase in the fluorescence lifetimes. These quenched intensities were due to energy transfer processes from the donor (Ru (bpy)3 2+) to the metal nanoparticles. Conversely, tryptophan showed enhanced fluorescence intensities with an increase in the size of the nanoparticles, which may be attributed to the effect of fluorescence resonance energy transfer and the overlap of the emission band of fluorophore with surface plasmon resonance bands of metallic nanoparticles. Phenylalanine, however, showed a decrease in fluorescence intensities in the presence of gold and silver nanoparticles. Thus the observed fluorescence properties of tris (2,2-bipyridyl) dichloro ruthenium (II) hexahydrate, tryptophan, and phenylalanine on silver and gold nanoparticles may be useful for the design of new analytical tools that can be used in the fields of labeling, biosensing, and bioimaging.</p>","abstract_has_math":false,"creators":["Kandimalla, Vamsi K."],"institution":null,"degree_name":"Master of Science (MS)","degree_level":"Open Access Thesis","degree_discipline":"Chemistry","degree_department":null,"school":null,"contributors":["Dr. Timothy Brewer","Dr. Maria Milletti","Dr. Vance Kennedy"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2010,"date_issued":"2010-01-01T08:00:00Z","date_published":"2010-01-01T08:00:00Z","updated_at":"2026-07-24T02:16:44Z","subjects":["Nanoparticle synthesis","fluorescence enhancement","tryptophan","tris(2","2-bipyridyl) dichloro ruthenium(II)","Chemistry"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://commons.emich.edu/theses/371","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Dr. Timothy Brewer","Dr. Maria Milletti","Dr. Vance Kennedy"]},{"key":"dc:creator","label":"Author","values":["Kandimalla, Vamsi K."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2012-01-23T08:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Chemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Open Access Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science (MS)"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Nanoparticle synthesis","fluorescence enhancement","tryptophan","tris(2","2-bipyridyl) dichloro ruthenium(II)","Chemistry"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://commons.emich.edu/theses/371"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["In this project, we studied the effect of silver and gold nanoparticles of various sizes on the fluorescence properties of tris (2, 2-bipyridyl) dichloro ruthenium (II) hexahydrate (Ru (bpy)3 2+), tryptophan and phenylalanine. For (Ru (bpy)3 2+) absorbed on silver and gold nanoparticles, the fluorescence intensity was quenched and showed a significant increase in the fluorescence lifetimes. These quenched intensities were due to energy transfer processes from the donor (Ru (bpy)3 2+) to the metal nanoparticles. Conversely, tryptophan showed enhanced fluorescence intensities with an increase in the size of the nanoparticles, which may be attributed to the effect of fluorescence resonance energy transfer and the overlap of the emission band of fluorophore with surface plasmon resonance bands of metallic nanoparticles. Phenylalanine, however, showed a decrease in fluorescence intensities in the presence of gold and silver nanoparticles. Thus the observed fluorescence properties of tris (2,2-bipyridyl) dichloro ruthenium (II) hexahydrate, tryptophan, and phenylalanine on silver and gold nanoparticles may be useful for the design of new analytical tools that can be used in the fields of labeling, biosensing, and bioimaging.
"]},{"key":"dc:title","label":"Title","values":["Influence of metal nanoparticles on fluorescence properties"]}]}],"canonical_facts":{"dc:contributor":["Dr. Timothy Brewer","Dr. Maria Milletti","Dr. Vance Kennedy"],"dc:creator":["Kandimalla, Vamsi K."],"dc:date.available":["2012-01-23T08:00:00Z"],"dc:description.abstract":["In this project, we studied the effect of silver and gold nanoparticles of various sizes on the fluorescence properties of tris (2, 2-bipyridyl) dichloro ruthenium (II) hexahydrate (Ru (bpy)3 2+), tryptophan and phenylalanine. For (Ru (bpy)3 2+) absorbed on silver and gold nanoparticles, the fluorescence intensity was quenched and showed a significant increase in the fluorescence lifetimes. These quenched intensities were due to energy transfer processes from the donor (Ru (bpy)3 2+) to the metal nanoparticles. Conversely, tryptophan showed enhanced fluorescence intensities with an increase in the size of the nanoparticles, which may be attributed to the effect of fluorescence resonance energy transfer and the overlap of the emission band of fluorophore with surface plasmon resonance bands of metallic nanoparticles. Phenylalanine, however, showed a decrease in fluorescence intensities in the presence of gold and silver nanoparticles. Thus the observed fluorescence properties of tris (2,2-bipyridyl) dichloro ruthenium (II) hexahydrate, tryptophan, and phenylalanine on silver and gold nanoparticles may be useful for the design of new analytical tools that can be used in the fields of labeling, biosensing, and bioimaging.
"],"dc:identifier":["https://commons.emich.edu/theses/371"],"dc:subject":["Nanoparticle synthesis","fluorescence enhancement","tryptophan","tris(2","2-bipyridyl) dichloro ruthenium(II)","Chemistry"],"dc:title":["Influence of metal nanoparticles on fluorescence properties"],"thesis:degree_discipline":["Chemistry"],"thesis:degree_level":["Open Access Thesis"],"thesis:degree_name":["Master of Science (MS)"]},"updated_at":"2026-07-24T02:16:44Z"}