Quantification of short-chain fatty acids in cecal material by gas chromatography-mass spectrometry

<p>Probiotic bacteria in the human colon that produce C₂ – C₄ short chain fatty acids (SCFA) and <em>Lactobacillus</em> species that produce lactate in addition to SCFAs are known to have positive health benefits. These organic acids were extracted with ether from murine cecal material (from Dr. Gary Huffnagle, Univ. of Mich.) and derivatized for quantitative measurement by gas chromatography-mass spectrometry. Antibiotic-treated animals gavaged with <em>Candidaalbicans</em> had a lower level of cecal butyrate relative to untreated animals, but a higher level of butyrate when treated mice were also administered <em>Lactobacillus johnsonii</em> NF-1. More recent studies have demonstrated a butyrate-lowering effect of antibiotic-/<em>C. albicans</em> treatment relative to antibiotic or <em>C. albicans</em> treatment alone, but a corresponding increase in the level of lactate. It is proposed that the steady state levels of small organic acids provide a marker of human health and disease.</p>

Name thesis:degree_name

Master of Science (MS)

Level thesis:degree_level

Open Access Thesis

Discipline thesis:degree_discipline

Chemistry

Year dc:date.available

2010

Author dc:creator

• Harrison, Charles

Contributors dc:contributor

• Steven Pernecky, PhD, chair
• Daniel Clemans, PhD
• Gavin Edwards, PhD

• short chain fatty acid analysis
• cecal material
• biological analysis
• gc/ms
• mouse models
• Chemistry

Repository record dc:identifier

https://commons.emich.edu/theses/277

OAI identifier oai:identifier

oai:commons.emich.edu:theses-1276