Using ethanol, a sustainable reversed-phase high-performance liquid chromatographic method with photo-diode array detection (HPLC-PDAD) was investigated to detect sub-parts per billion concentrations of N-nitrosodimethylamine (NDMA) in drinking water and environmental water samples. This presents a practical and “green” option to analytical methods that require laborious derivatization, expensive detectors and unecological solvents.
Mobile phase characteristics, instrument operating parameters, and sample preparation were investigated. Selectivity for NDMA was evaluated using three different C18 columns. Optimal peak resolution and detection were achieved at 231 nm. The limit of detection and quantitation for NDMA was 0.29 μg/mL and 0.96 μg/mL, respectively, with an ethanol modifier and 5-μm column. A sustainable solid-phase extraction method was also investigated. Using ethanol for cartridge conditioning and extraction, recovery of NDMA was low (10%).
","abstract_html":"<p>Using ethanol, a sustainable reversed-phase high-performance liquid chromatographic method with photo-diode array detection (HPLC-PDAD) was investigated to detect sub-parts per billion concentrations of N-nitrosodimethylamine (NDMA) in drinking water and environmental water samples. This presents a practical and “green” option to analytical methods that require laborious derivatization, expensive detectors and unecological solvents.</p> <p>Mobile phase characteristics, instrument operating parameters, and sample preparation were investigated. Selectivity for NDMA was evaluated using three different C18 columns. Optimal peak resolution and detection were achieved at 231 nm. The limit of detection and quantitation for NDMA was 0.29 μg/mL and 0.96 μg/mL, respectively, with an ethanol modifier and 5-μm column. A sustainable solid-phase extraction method was also investigated. Using ethanol for cartridge conditioning and extraction, recovery of NDMA was low (10%).</p>","abstract_has_math":false,"creators":["McDay, JoLisa"],"institution":null,"degree_name":"Master of Science (MS)","degree_level":"Open Access Thesis","degree_discipline":"Chemistry","degree_department":null,"school":null,"contributors":["Heather Holmes, Ph.D, Chair","Harriet Lindsay, Ph.D.","Donald Snyder, Ph.D."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2010,"date_issued":"2010-01-01T08:00:00Z","date_published":"2010-01-01T08:00:00Z","updated_at":"2026-07-24T02:16:36Z","subjects":["Dimethylnitrosamine Toxicity testing","Environmental testing","Alcohol","Extraction (Chemistry)","Water Analysis Research","Chemistry"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://commons.emich.edu/theses/260","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Heather Holmes, Ph.D, Chair","Harriet Lindsay, Ph.D.","Donald Snyder, Ph.D."]},{"key":"dc:creator","label":"Author","values":["McDay, JoLisa"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"thesis:degree_discipline","label":"Discipline","values":["Chemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Open Access Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science (MS)"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Dimethylnitrosamine Toxicity testing","Environmental testing","Alcohol","Extraction (Chemistry)","Water Analysis Research","Chemistry"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://commons.emich.edu/theses/260"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Using ethanol, a sustainable reversed-phase high-performance liquid chromatographic method with photo-diode array detection (HPLC-PDAD) was investigated to detect sub-parts per billion concentrations of N-nitrosodimethylamine (NDMA) in drinking water and environmental water samples. This presents a practical and “green” option to analytical methods that require laborious derivatization, expensive detectors and unecological solvents.
Mobile phase characteristics, instrument operating parameters, and sample preparation were investigated. Selectivity for NDMA was evaluated using three different C18 columns. Optimal peak resolution and detection were achieved at 231 nm. The limit of detection and quantitation for NDMA was 0.29 μg/mL and 0.96 μg/mL, respectively, with an ethanol modifier and 5-μm column. A sustainable solid-phase extraction method was also investigated. Using ethanol for cartridge conditioning and extraction, recovery of NDMA was low (10%).
"]},{"key":"dc:title","label":"Title","values":["Ecological method development for detecting N-nitrosodimethylamine in water using HPLC-PDAD"]}]}],"canonical_facts":{"dc:contributor":["Heather Holmes, Ph.D, Chair","Harriet Lindsay, Ph.D.","Donald Snyder, Ph.D."],"dc:creator":["McDay, JoLisa"],"dc:description.abstract":["Using ethanol, a sustainable reversed-phase high-performance liquid chromatographic method with photo-diode array detection (HPLC-PDAD) was investigated to detect sub-parts per billion concentrations of N-nitrosodimethylamine (NDMA) in drinking water and environmental water samples. This presents a practical and “green” option to analytical methods that require laborious derivatization, expensive detectors and unecological solvents.
Mobile phase characteristics, instrument operating parameters, and sample preparation were investigated. Selectivity for NDMA was evaluated using three different C18 columns. Optimal peak resolution and detection were achieved at 231 nm. The limit of detection and quantitation for NDMA was 0.29 μg/mL and 0.96 μg/mL, respectively, with an ethanol modifier and 5-μm column. A sustainable solid-phase extraction method was also investigated. Using ethanol for cartridge conditioning and extraction, recovery of NDMA was low (10%).
"],"dc:identifier":["https://commons.emich.edu/theses/260"],"dc:subject":["Dimethylnitrosamine Toxicity testing","Environmental testing","Alcohol","Extraction (Chemistry)","Water Analysis Research","Chemistry"],"dc:title":["Ecological method development for detecting N-nitrosodimethylamine in water using HPLC-PDAD"],"thesis:degree_discipline":["Chemistry"],"thesis:degree_level":["Open Access Thesis"],"thesis:degree_name":["Master of Science (MS)"]},"updated_at":"2026-07-24T02:16:36Z"}