Measurement of lipid peroxidation in biology models using gas-chromatography-aass spectrometry

<p>C2C12 mouse myoblast cells, grown in glass vials, were connected to a cryofocusing unit to trap volatile organic compounds (VOCs). The VOCs were eluted from the trap by capacitive discharge into a gas chromatograph with time-of-flight mass spectral capabilities (GC-TOFMS) and were found to include the lipid peroxidation product hexanal. The pro-oxidant cumene hydroperoxide elevated the levels of these lipid peroxidation products, whereas the anti-oxidant butylated hydroxy toluene (BHT) impaired their production. Derivatization of the aldehyde products of lipid peroxidation in the same myoblast cells with pentafluorobenzyl hydroxylamine hydrochloride (PFB) provided evidence for formation of non-volatile products of lipid peroxidation such as malondialdehyde and 4hydroxynonenal.</p> <p>Similar experiments with the human tracheal epithelial cells, 9-HTE cells treated with <em>Haemophilus influenza</em> bacteria, showed elevated levels of malondialdehyde at 8- hour incubation time intervals giving the initial evidence that the products of lipid peroxidation are formed long before the COX-1 enzyme is activated.</p>

Name thesis:degree_name

Master of Science (MS)

Level thesis:degree_level

Open Access Thesis

Discipline thesis:degree_discipline

Chemistry

Year

2005

Author dc:creator

• Lokireddy, Madhavi

Contributors dc:contributor

• Dr. Steven Pernecky
• Dr. Heather Holmes
• Dr. Hedeel Guy Evans

• Mass spectrometry Instruments
• Gas chromatography Instruments
• Volatile organic compounds
• Myoblasts
• Peroxidation Measurement
• Lipids Measurement
• Chemistry

Repository record dc:identifier

https://commons.emich.edu/theses/113

OAI identifier oai:identifier

oai:commons.emich.edu:theses-1112