Listeria Monocytogenes (L. monocytogenes) is a ubiquitous bacterium that can be found in both soil and water. This opportunistic intercellular pathogen is often food-borne and can lead to serious illness or death of those that are immunocompromised. Current medical research into the practical use of this pathogen has revealed that the intercellular mechanisms can be exploited to produce a cancer vaccine. Currently L. monocytogenes is being studied as a viable candidate for various other cancer vaccines and therapies. The purpose of this study is to reduce the downstream virulence factors that heavily rely on upregulation of the prfA gene. In this paper the pIMC plasmid is used in an attempt to knock-out the untranslated region (UTR) of the prfA gene located on the LIPI-1 pathogenicity island of the L. monocytogenes.
","abstract_html":"<p>Listeria Monocytogenes (L. monocytogenes) is a ubiquitous bacterium that can be found in both soil and water. This opportunistic intercellular pathogen is often food-borne and can lead to serious illness or death of those that are immunocompromised. Current medical research into the practical use of this pathogen has revealed that the intercellular mechanisms can be exploited to produce a cancer vaccine. Currently L. monocytogenes is being studied as a viable candidate for various other cancer vaccines and therapies. The purpose of this study is to reduce the downstream virulence factors that heavily rely on upregulation of the prfA gene. In this paper the pIMC plasmid is used in an attempt to knock-out the untranslated region (UTR) of the prfA gene located on the LIPI-1 pathogenicity island of the L. monocytogenes.</p>","abstract_has_math":false,"creators":["Jackson, Robert"],"institution":"Eastern Kentucky University","degree_name":"Master of Science (MS)","degree_level":"Master's","degree_discipline":"Biological Sciences","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2019,"date_issued":"2019-01-01T08:00:00Z","date_published":"2019-01-01T08:00:00Z","updated_at":"2026-07-24T02:15:53Z","subjects":["Cancer Biology"],"languages":[],"rights":["Copyright 2019 Robert Jackson"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://encompass.eku.edu/etd/610","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Jackson, Robert"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:publisher","label":"Institution","values":["Encompass Digital Archive, Eastern Kentucky University"]},{"key":"dc:type","label":"Dc Type","values":["Master Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biological Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Master's"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science (MS)"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["Eastern Kentucky University"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Cancer Biology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2019 Robert Jackson"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://encompass.eku.edu/etd/610"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Listeria Monocytogenes (L. monocytogenes) is a ubiquitous bacterium that can be found in both soil and water. This opportunistic intercellular pathogen is often food-borne and can lead to serious illness or death of those that are immunocompromised. Current medical research into the practical use of this pathogen has revealed that the intercellular mechanisms can be exploited to produce a cancer vaccine. Currently L. monocytogenes is being studied as a viable candidate for various other cancer vaccines and therapies. The purpose of this study is to reduce the downstream virulence factors that heavily rely on upregulation of the prfA gene. In this paper the pIMC plasmid is used in an attempt to knock-out the untranslated region (UTR) of the prfA gene located on the LIPI-1 pathogenicity island of the L. monocytogenes.
"]},{"key":"dc:format","label":"Dc Format","values":["application/PDF"]},{"key":"dc:source","label":"Dc Source","values":["Encompass Digital Archive: Online Theses and Dissertations"]},{"key":"dc:title","label":"Title","values":["Knockout of the trans-acting riboswitches in Listeria monocytogenes to disrupt downstream virulence factors"]}]}],"canonical_facts":{"dc:creator":["Jackson, Robert"],"dc:description.abstract":["Listeria Monocytogenes (L. monocytogenes) is a ubiquitous bacterium that can be found in both soil and water. This opportunistic intercellular pathogen is often food-borne and can lead to serious illness or death of those that are immunocompromised. Current medical research into the practical use of this pathogen has revealed that the intercellular mechanisms can be exploited to produce a cancer vaccine. Currently L. monocytogenes is being studied as a viable candidate for various other cancer vaccines and therapies. The purpose of this study is to reduce the downstream virulence factors that heavily rely on upregulation of the prfA gene. In this paper the pIMC plasmid is used in an attempt to knock-out the untranslated region (UTR) of the prfA gene located on the LIPI-1 pathogenicity island of the L. monocytogenes.
"],"dc:format":["application/PDF"],"dc:identifier":["https://encompass.eku.edu/etd/610"],"dc:publisher":["Encompass Digital Archive, Eastern Kentucky University"],"dc:rights":["Copyright 2019 Robert Jackson"],"dc:source":["Encompass Digital Archive: Online Theses and Dissertations"],"dc:subject":["Cancer Biology"],"dc:title":["Knockout of the trans-acting riboswitches in Listeria monocytogenes to disrupt downstream virulence factors"],"dc:type":["Master Thesis"],"thesis:degree_discipline":["Biological Sciences"],"thesis:degree_level":["Master's"],"thesis:degree_name":["Master of Science (MS)"],"thesis:institution_name":["Eastern Kentucky University"]},"updated_at":"2026-07-24T02:15:53Z"}