An important aspect of conservation biology is understanding how land-use changes impact biodiversity. Ridge-top wetlands are unique habitat for pond-breeding amphibians and the Daniel Boone National Forest (DBNF) contains natural forested ridge-top wetlands in close proximity to constructed wetlands intermixed across the same landscape. Genetic data can be used to address current population status, probability of persistence, and population connectivity. The objective of this study was to determine the amount and distribution of population genetic diversity of wood frogs in natural ridge-top wetlands and what factors influence this. Genetic data were analyzed for nine microsatellite DNA loci from twenty-five wood frog egg clutches at each of five randomly selected natural wetlands. Overall, genetic variation was measured by calculating observed heterozygosity (0.250-0.960), expected heterozygosity (0.270-0.913), and mean allelic richness (8.83-11.95). The results from program STRUCTURE gave support for 3 genetic clusters, and overall FST was 0.054 ± 0.022 SE among populations. Three populations exhibited signs of a recent population bottleneck event within populations. Pairwise FST and DST values were correlated, with DST indicating slightly higher population divergence. Isolation by distance was significant (P = 0.0354; R2 = 0.445), indicating that geographic distance between the wetlands was an important factor explaining genetic differentiation. Future work should focus on expanding the sampling to a larger scale and sampling both natural and constructed wetlands between the sites to understand more fully how the genetic variation is partitioned across the landscape. For example, wood frogs have been observed breeding in both constructed and natural wetlands, which may demonstrate source/sink dynamics, and predation on wood frog eggs in constructed wetlands may decrease overall wood frog genetic diversity over time.
","abstract_html":"<p>An important aspect of conservation biology is understanding how land-use changes impact biodiversity. Ridge-top wetlands are unique habitat for pond-breeding amphibians and the Daniel Boone National Forest (DBNF) contains natural forested ridge-top wetlands in close proximity to constructed wetlands intermixed across the same landscape. Genetic data can be used to address current population status, probability of persistence, and population connectivity. The objective of this study was to determine the amount and distribution of population genetic diversity of wood frogs in natural ridge-top wetlands and what factors influence this. Genetic data were analyzed for nine microsatellite DNA loci from twenty-five wood frog egg clutches at each of five randomly selected natural wetlands. Overall, genetic variation was measured by calculating observed heterozygosity (0.250-0.960), expected heterozygosity (0.270-0.913), and mean allelic richness (8.83-11.95). The results from program STRUCTURE gave support for 3 genetic clusters, and overall FST was 0.054 ± 0.022 SE among populations. Three populations exhibited signs of a recent population bottleneck event within populations. Pairwise FST and DST values were correlated, with DST indicating slightly higher population divergence. Isolation by distance was significant (P = 0.0354; R2 = 0.445), indicating that geographic distance between the wetlands was an important factor explaining genetic differentiation. Future work should focus on expanding the sampling to a larger scale and sampling both natural and constructed wetlands between the sites to understand more fully how the genetic variation is partitioned across the landscape. For example, wood frogs have been observed breeding in both constructed and natural wetlands, which may demonstrate source/sink dynamics, and predation on wood frog eggs in constructed wetlands may decrease overall wood frog genetic diversity over time. </p>","abstract_has_math":false,"creators":["Strong, Jennifer R."],"institution":"Eastern Kentucky University","degree_name":"Master of Science (MS)","degree_level":"Master's","degree_discipline":"Biological Sciences","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2014,"date_issued":"2014-01-01T08:00:00Z","date_published":"2014-01-01T08:00:00Z","updated_at":"2026-07-24T02:15:32Z","subjects":["Amphibians","Lithobates sylvaticus","Microsatellites","Population genetics","Wetland","Wood frogs","Terrestrial and Aquatic Ecology"],"languages":[],"rights":["Copyright 2014 Jennifer R. Strong"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://encompass.eku.edu/etd/319","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Strong, Jennifer R."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:publisher","label":"Institution","values":["Encompass Digital Archive, Eastern Kentucky University"]},{"key":"dc:type","label":"Dc Type","values":["Master Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biological Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Master's"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science (MS)"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["Eastern Kentucky University"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Amphibians","Lithobates sylvaticus","Microsatellites","Population genetics","Wetland","Wood frogs","Terrestrial and Aquatic Ecology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2014 Jennifer R. Strong"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://encompass.eku.edu/etd/319"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["An important aspect of conservation biology is understanding how land-use changes impact biodiversity. Ridge-top wetlands are unique habitat for pond-breeding amphibians and the Daniel Boone National Forest (DBNF) contains natural forested ridge-top wetlands in close proximity to constructed wetlands intermixed across the same landscape. Genetic data can be used to address current population status, probability of persistence, and population connectivity. The objective of this study was to determine the amount and distribution of population genetic diversity of wood frogs in natural ridge-top wetlands and what factors influence this. Genetic data were analyzed for nine microsatellite DNA loci from twenty-five wood frog egg clutches at each of five randomly selected natural wetlands. Overall, genetic variation was measured by calculating observed heterozygosity (0.250-0.960), expected heterozygosity (0.270-0.913), and mean allelic richness (8.83-11.95). The results from program STRUCTURE gave support for 3 genetic clusters, and overall FST was 0.054 ± 0.022 SE among populations. Three populations exhibited signs of a recent population bottleneck event within populations. Pairwise FST and DST values were correlated, with DST indicating slightly higher population divergence. Isolation by distance was significant (P = 0.0354; R2 = 0.445), indicating that geographic distance between the wetlands was an important factor explaining genetic differentiation. Future work should focus on expanding the sampling to a larger scale and sampling both natural and constructed wetlands between the sites to understand more fully how the genetic variation is partitioned across the landscape. For example, wood frogs have been observed breeding in both constructed and natural wetlands, which may demonstrate source/sink dynamics, and predation on wood frog eggs in constructed wetlands may decrease overall wood frog genetic diversity over time.
"]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:source","label":"Dc Source","values":["Encompass Digital Archive: Online Theses and Dissertations"]},{"key":"dc:title","label":"Title","values":["Population genetics of wood frogs (Lithobates sylvaticus) in a forested ridge-top wetland ecosystem"]}]}],"canonical_facts":{"dc:creator":["Strong, Jennifer R."],"dc:description.abstract":["An important aspect of conservation biology is understanding how land-use changes impact biodiversity. Ridge-top wetlands are unique habitat for pond-breeding amphibians and the Daniel Boone National Forest (DBNF) contains natural forested ridge-top wetlands in close proximity to constructed wetlands intermixed across the same landscape. Genetic data can be used to address current population status, probability of persistence, and population connectivity. The objective of this study was to determine the amount and distribution of population genetic diversity of wood frogs in natural ridge-top wetlands and what factors influence this. Genetic data were analyzed for nine microsatellite DNA loci from twenty-five wood frog egg clutches at each of five randomly selected natural wetlands. Overall, genetic variation was measured by calculating observed heterozygosity (0.250-0.960), expected heterozygosity (0.270-0.913), and mean allelic richness (8.83-11.95). The results from program STRUCTURE gave support for 3 genetic clusters, and overall FST was 0.054 ± 0.022 SE among populations. Three populations exhibited signs of a recent population bottleneck event within populations. Pairwise FST and DST values were correlated, with DST indicating slightly higher population divergence. Isolation by distance was significant (P = 0.0354; R2 = 0.445), indicating that geographic distance between the wetlands was an important factor explaining genetic differentiation. Future work should focus on expanding the sampling to a larger scale and sampling both natural and constructed wetlands between the sites to understand more fully how the genetic variation is partitioned across the landscape. For example, wood frogs have been observed breeding in both constructed and natural wetlands, which may demonstrate source/sink dynamics, and predation on wood frog eggs in constructed wetlands may decrease overall wood frog genetic diversity over time.
"],"dc:format":["application/pdf"],"dc:identifier":["https://encompass.eku.edu/etd/319"],"dc:publisher":["Encompass Digital Archive, Eastern Kentucky University"],"dc:rights":["Copyright 2014 Jennifer R. Strong"],"dc:source":["Encompass Digital Archive: Online Theses and Dissertations"],"dc:subject":["Amphibians","Lithobates sylvaticus","Microsatellites","Population genetics","Wetland","Wood frogs","Terrestrial and Aquatic Ecology"],"dc:title":["Population genetics of wood frogs (Lithobates sylvaticus) in a forested ridge-top wetland ecosystem"],"dc:type":["Master Thesis"],"thesis:degree_discipline":["Biological Sciences"],"thesis:degree_level":["Master's"],"thesis:degree_name":["Master of Science (MS)"],"thesis:institution_name":["Eastern Kentucky University"]},"updated_at":"2026-07-24T02:15:32Z"}