Preparation And Characterization Of Self-Assembled Monolayers And Mesoscale Protein Patterning

<p>Bottom-up approach was used to develop self-assembled monolayers of octadecyltrichlorosilane (OTS) and undecenyltrichlorosilane(UTS) on Si(100) wafer. Undecenyltrichlorosilane monolayer was oxidized at the vinyl terminal to generate a carboxylic acid group. Lysozyme protein was immobilized on the polar carboxylic acid group. The developed protein patterns were investigated using fluorescence microscopy. Lysozyme has an isoelectronic point of 11.35. At a pH below this value the protein is positively charged making it a good candidate for electrostatic adsorption on the negatively charge -COO- group. Fluorescence images confirm formation of lysozyme across the silicon wafer. The patterned Si(100) wafer can be used as a biosensor against lysozyme antibodies. </p> <p>Another approach to develop varied surface properties was used to grow OTS on oxidized UTSox via chemical phase deposition (CVD). In this case we used polystyrene and silicon nanospheres as masking agents on the already developed and oxidized UTS. Fluorescence images revealed that OTS layers were formed on the interstitial spaces of the nanosphere masks. Varied protein can be immobilized on this surface due to different terminal groups on the surface. </p>

Name thesis:degree_name

Master of Science (MS)

Level thesis:degree_level

Master's

Discipline thesis:degree_discipline

Chemistry

Grantor dc:publisher

Eastern Kentucky University

Year

2015

Author dc:creator

• Noomuna, Panae

• Fluorescence Microscopy
• Lysozyme
• Mesoscale Protein Patterns
• Nanosphere Lithography
• Self-Assembled Monolayers
• Chemistry

Statement dc:rights

• Copyright 2015 Panae Noomuna

Repository record dc:identifier

https://encompass.eku.edu/etd/300

OAI identifier oai:identifier

oai:encompass.eku.edu:etd-1298