\"Fertilizer grade biosolid is sold to the general public by the Cheney Wastewater Treatment Plant for use as garden fertilizer and as a soil conditioner. Moist biosolid samples were obtained from the Cheney Wastewater Treatment Plant and antibiotic resistant gram negative bacteria were isolated from it. Bacteria isolated from these samples were resistant to tetracycline, ampicillin, streptomycin, kanamycin and chloramphenicol. Tetracycline resistant bacteria were isolated on MacConkey's agar supplemented with tetracycline and their resistance to ampicillin, streptomycin, chloramphenicol and kanamycin were also determined. A total of 48 isolates cultivated to determine the presence of plasmids which often contain antibiotic resistance genes. These isolates were identified using the Vitek2 Identification System (BioMerieux, Durham N.C; U.S.A.). Sixty-seven percent of isolates were discovered to have plasmids and 54% had two or more plasmids. Plasmid size ranged from 2 to lOKb\"--Document.
","abstract_html":"<p>"Fertilizer grade biosolid is sold to the general public by the Cheney Wastewater Treatment Plant for use as garden fertilizer and as a soil conditioner. Moist biosolid samples were obtained from the Cheney Wastewater Treatment Plant and antibiotic resistant gram negative bacteria were isolated from it. Bacteria isolated from these samples were resistant to tetracycline, ampicillin, streptomycin, kanamycin and chloramphenicol. Tetracycline resistant bacteria were isolated on MacConkey's agar supplemented with tetracycline and their resistance to ampicillin, streptomycin, chloramphenicol and kanamycin were also determined. A total of 48 isolates cultivated to determine the presence of plasmids which often contain antibiotic resistance genes. These isolates were identified using the Vitek2 Identification System (BioMerieux, Durham N.C; U.S.A.). Sixty-seven percent of isolates were discovered to have plasmids and 54% had two or more plasmids. Plasmid size ranged from 2 to lOKb"--Document.</p>","abstract_has_math":false,"creators":["Hartman, Gwendolyn Lenore"],"institution":null,"degree_name":"Master of Science (MS) in Biology","degree_level":"Thesis","degree_discipline":"Biology","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2012,"date_issued":"2012-01-01T08:00:00Z","date_published":"2012-01-01T08:00:00Z","updated_at":"2026-07-24T02:13:00Z","subjects":["Cheney Wastewater Treatment Plant","Drug resistance in microorganisms--Washington (State)--Cheney","Sewage sludge as fertilizer--Health aspects--Washington (State)--Cheney","Sewage sludge as fertilizer--Toxicology--Washington (State)--Cheney","Biology"],"languages":[],"rights":["Access is available to all users"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://dc.ewu.edu/theses/77","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Hartman, Gwendolyn Lenore"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"thesis:degree_discipline","label":"Discipline","values":["Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science (MS) in Biology"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Cheney Wastewater Treatment Plant","Drug resistance in microorganisms--Washington (State)--Cheney","Sewage sludge as fertilizer--Health aspects--Washington (State)--Cheney","Sewage sludge as fertilizer--Toxicology--Washington (State)--Cheney","Biology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["Access is available to all users"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://dc.ewu.edu/theses/77"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["\"Fertilizer grade biosolid is sold to the general public by the Cheney Wastewater Treatment Plant for use as garden fertilizer and as a soil conditioner. Moist biosolid samples were obtained from the Cheney Wastewater Treatment Plant and antibiotic resistant gram negative bacteria were isolated from it. Bacteria isolated from these samples were resistant to tetracycline, ampicillin, streptomycin, kanamycin and chloramphenicol. Tetracycline resistant bacteria were isolated on MacConkey's agar supplemented with tetracycline and their resistance to ampicillin, streptomycin, chloramphenicol and kanamycin were also determined. A total of 48 isolates cultivated to determine the presence of plasmids which often contain antibiotic resistance genes. These isolates were identified using the Vitek2 Identification System (BioMerieux, Durham N.C; U.S.A.). Sixty-seven percent of isolates were discovered to have plasmids and 54% had two or more plasmids. Plasmid size ranged from 2 to lOKb\"--Document.
"]},{"key":"dc:title","label":"Title","values":["An assessment of antibiotic resistant bacteria in biosolid used as garden fertilizer"]}]}],"canonical_facts":{"dc:creator":["Hartman, Gwendolyn Lenore"],"dc:description.abstract":["\"Fertilizer grade biosolid is sold to the general public by the Cheney Wastewater Treatment Plant for use as garden fertilizer and as a soil conditioner. Moist biosolid samples were obtained from the Cheney Wastewater Treatment Plant and antibiotic resistant gram negative bacteria were isolated from it. Bacteria isolated from these samples were resistant to tetracycline, ampicillin, streptomycin, kanamycin and chloramphenicol. Tetracycline resistant bacteria were isolated on MacConkey's agar supplemented with tetracycline and their resistance to ampicillin, streptomycin, chloramphenicol and kanamycin were also determined. A total of 48 isolates cultivated to determine the presence of plasmids which often contain antibiotic resistance genes. These isolates were identified using the Vitek2 Identification System (BioMerieux, Durham N.C; U.S.A.). Sixty-seven percent of isolates were discovered to have plasmids and 54% had two or more plasmids. Plasmid size ranged from 2 to lOKb\"--Document.
"],"dc:identifier":["https://dc.ewu.edu/theses/77"],"dc:rights":["Access is available to all users"],"dc:subject":["Cheney Wastewater Treatment Plant","Drug resistance in microorganisms--Washington (State)--Cheney","Sewage sludge as fertilizer--Health aspects--Washington (State)--Cheney","Sewage sludge as fertilizer--Toxicology--Washington (State)--Cheney","Biology"],"dc:title":["An assessment of antibiotic resistant bacteria in biosolid used as garden fertilizer"],"thesis:degree_discipline":["Biology"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["Master of Science (MS) in Biology"]},"updated_at":"2026-07-24T02:13:00Z"}