{"id":{"repo_id":"east-anglia","oai_identifier":"oai:ueaeprints.uea.ac.uk:59214"},"canonical_url":"https://search.dev.ndltd.org/etd/east-anglia/oai:ueaeprints.uea.ac.uk:59214","repository":{"repo_id":"east-anglia","name":"University of East Anglia","base_url":"https://ueaeprints.uea.ac.uk/cgi/oai2"},"display":{"title":"The role of international ‘high-risk clones’ in the emergence of metallo-b-lactamase-producing pseudomonas aeruginosa in the UK","abstract":"Pseudomonas aeruginosa is a Gram-negative bacterium responsible for a wide range of opportunistic hospital-acquired infections. However metallo-β-lactamases (MBLs) encoding resistance to carbapenems and other β-lactam antibiotics – which are important agents for the treatment of P. aeruginosa infections – are increasingly reported in the species worldwide. This study aimed to characterise a comprehensive collection of MBL-producing P. aeruginosa referred to the UK national reference laboratory from 267 patients between 2003 and 2012. VIM-type MBLs predominated (91%) but a few IMP- and NDM-type enzymes were also identified. Diverse types were identified amongst these isolates using nine-locus variable number tandem-repeat (VNTR) analysis, but the majority (85%) belonged to six major complexes, corresponding to sequence types (STs) 111, 233, 235, 357,654/964 and 773, respectively, by multi-locus sequence typing (MLST). These are all‘high-risk clones’ frequently reported internationally as hosts of MBLs. blaVIM- and blaIMP-MBL genes were carried in diverse class 1 integron structures; the six most common integron structures (I-VI) carried blaVIM-2 (n=5) or blaVIM-6 (n=1) genes; four were associated with single STs (I, ST111; III, ST773; IV, ST654/964; V,ST357) while two integrons types (II,VI) were seen in multiple STs. Five of these six integrons were located on the P. aeruginosa chromosome, while one (VI) was located on a plasmid. VIM-encoding genomic islands were characterised in representatives of STs 111, 233 and 235 using whole genome sequencing (WGS) methods. SNP analysis achieved greater discrimination among MBL-producing representatives of STs 111 and 235. CRISPR-Cas bacterial defence systems were absent from all sequenced representatives of STs 111 and 235 but were present in single representatives of STs 233, 357, 654 and 773. Overall, ‘high-risk clones’ are important in the spread of MBL-producing P. aeruginosa in the UK. Future work should focus on further elucidating the reasons for the success of these clones, to better understand how to limit the spread of these multi-drug resistant organisms.","abstract_html":"Pseudomonas aeruginosa is a Gram-negative bacterium responsible for a wide range of opportunistic hospital-acquired infections. However metallo-β-lactamases (MBLs) encoding resistance to carbapenems and other β-lactam antibiotics – which are important agents for the treatment of P. aeruginosa infections – are increasingly reported in the species worldwide. This study aimed to characterise a comprehensive collection of MBL-producing P. aeruginosa referred to the UK national reference laboratory from 267 patients between 2003 and 2012. VIM-type MBLs predominated (91%) but a few IMP- and NDM-type enzymes were also identified. Diverse types were identified amongst these isolates using nine-locus variable number tandem-repeat (VNTR) analysis, but the majority (85%) belonged to six major complexes, corresponding to sequence types (STs) 111, 233, 235, 357,654/964 and 773, respectively, by multi-locus sequence typing (MLST). These are all‘high-risk clones’ frequently reported internationally as hosts of MBLs. blaVIM- and blaIMP-MBL genes were carried in diverse class 1 integron structures; the six most common integron structures (I-VI) carried blaVIM-2 (n=5) or blaVIM-6 (n=1) genes; four were associated with single STs (I, ST111; III, ST773; IV, ST654/964; V,ST357) while two integrons types (II,VI) were seen in multiple STs. Five of these six integrons were located on the P. aeruginosa chromosome, while one (VI) was located on a plasmid. VIM-encoding genomic islands were characterised in representatives of STs 111, 233 and 235 using whole genome sequencing (WGS) methods. SNP analysis achieved greater discrimination among MBL-producing representatives of STs 111 and 235. CRISPR-Cas bacterial defence systems were absent from all sequenced representatives of STs 111 and 235 but were present in single representatives of STs 233, 357, 654 and 773. Overall, ‘high-risk clones’ are important in the spread of MBL-producing P. aeruginosa in the UK. Future work should focus on further elucidating the reasons for the success of these clones, to better understand how to limit the spread of these multi-drug resistant organisms.","abstract_has_math":false,"creators":["Wright, Laura L."],"institution":"University of East Anglia","degree_name":"phd","degree_level":"doctoral","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-12","date_published":"2015-12","updated_at":"2026-07-24T02:12:16Z","subjects":[],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":null,"outbound_label":null,"outbound_source":null},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Wright, Laura L."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-12"]},{"key":"dc:date.issued","label":"Date","values":["2015-12"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["Norwich Medical School"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["University of East Anglia"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://ueaeprints.uea.ac.uk/id/eprint/59214/"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["doctoral"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["phd"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://ueaeprints.uea.ac.uk/id/eprint/59214/1/2015WrightLLPhD.pdf"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Pseudomonas aeruginosa is a Gram-negative bacterium responsible for a wide range of opportunistic hospital-acquired infections. However metallo-β-lactamases (MBLs) encoding resistance to carbapenems and other β-lactam antibiotics – which are important agents for the treatment of P. aeruginosa infections – are increasingly reported in the species worldwide. This study aimed to characterise a comprehensive collection of MBL-producing P. aeruginosa referred to the UK national reference laboratory from 267 patients between 2003 and 2012. VIM-type MBLs predominated (91%) but a few IMP- and NDM-type enzymes were also identified. Diverse types were identified amongst these isolates using nine-locus variable number tandem-repeat (VNTR) analysis, but the majority (85%) belonged to six major complexes, corresponding to sequence types (STs) 111, 233, 235, 357,654/964 and 773, respectively, by multi-locus sequence typing (MLST). These are all‘high-risk clones’ frequently reported internationally as hosts of MBLs. blaVIM- and blaIMP-MBL genes were carried in diverse class 1 integron structures; the six most common integron structures (I-VI) carried blaVIM-2 (n=5) or blaVIM-6 (n=1) genes; four were associated with single STs (I, ST111; III, ST773; IV, ST654/964; V,ST357) while two integrons types (II,VI) were seen in multiple STs. Five of these six integrons were located on the P. aeruginosa chromosome, while one (VI) was located on a plasmid. VIM-encoding genomic islands were characterised in representatives of STs 111, 233 and 235 using whole genome sequencing (WGS) methods. SNP analysis achieved greater discrimination among MBL-producing representatives of STs 111 and 235. CRISPR-Cas bacterial defence systems were absent from all sequenced representatives of STs 111 and 235 but were present in single representatives of STs 233, 357, 654 and 773. Overall, ‘high-risk clones’ are important in the spread of MBL-producing P. aeruginosa in the UK. Future work should focus on further elucidating the reasons for the success of these clones, to better understand how to limit the spread of these multi-drug resistant organisms."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["The role of international ‘high-risk clones’ in the emergence of metallo-b-lactamase-producing pseudomonas aeruginosa in the UK"]}]}],"canonical_facts":{"dc:creator":["Wright, Laura L."],"dc:date":["2015-12"],"dc:date.issued":["2015-12"],"dc:description.abstract":["Pseudomonas aeruginosa is a Gram-negative bacterium responsible for a wide range of opportunistic hospital-acquired infections. However metallo-β-lactamases (MBLs) encoding resistance to carbapenems and other β-lactam antibiotics – which are important agents for the treatment of P. aeruginosa infections – are increasingly reported in the species worldwide. This study aimed to characterise a comprehensive collection of MBL-producing P. aeruginosa referred to the UK national reference laboratory from 267 patients between 2003 and 2012. VIM-type MBLs predominated (91%) but a few IMP- and NDM-type enzymes were also identified. Diverse types were identified amongst these isolates using nine-locus variable number tandem-repeat (VNTR) analysis, but the majority (85%) belonged to six major complexes, corresponding to sequence types (STs) 111, 233, 235, 357,654/964 and 773, respectively, by multi-locus sequence typing (MLST). These are all‘high-risk clones’ frequently reported internationally as hosts of MBLs. blaVIM- and blaIMP-MBL genes were carried in diverse class 1 integron structures; the six most common integron structures (I-VI) carried blaVIM-2 (n=5) or blaVIM-6 (n=1) genes; four were associated with single STs (I, ST111; III, ST773; IV, ST654/964; V,ST357) while two integrons types (II,VI) were seen in multiple STs. Five of these six integrons were located on the P. aeruginosa chromosome, while one (VI) was located on a plasmid. VIM-encoding genomic islands were characterised in representatives of STs 111, 233 and 235 using whole genome sequencing (WGS) methods. SNP analysis achieved greater discrimination among MBL-producing representatives of STs 111 and 235. CRISPR-Cas bacterial defence systems were absent from all sequenced representatives of STs 111 and 235 but were present in single representatives of STs 233, 357, 654 and 773. Overall, ‘high-risk clones’ are important in the spread of MBL-producing P. aeruginosa in the UK. Future work should focus on further elucidating the reasons for the success of these clones, to better understand how to limit the spread of these multi-drug resistant organisms."],"dc:format":["application/pdf"],"dc:identifier.uri":["https://ueaeprints.uea.ac.uk/id/eprint/59214/1/2015WrightLLPhD.pdf"],"dc:language":["en"],"dc:publisher.department":["Norwich Medical School"],"dc:publisher.institution":["University of East Anglia"],"dc:relation.isreferencedby":["https://ueaeprints.uea.ac.uk/id/eprint/59214/"],"dc:title":["The role of international ‘high-risk clones’ in the emergence of metallo-b-lactamase-producing pseudomonas aeruginosa in the UK"],"dc:type":["Thesis"],"dc:type.qualificationlevel":["doctoral"],"dc:type.qualificationname":["phd"]},"updated_at":"2026-07-24T02:12:16Z"}