{"id":{"repo_id":"east-anglia","oai_identifier":"oai:ueaeprints.uea.ac.uk:57216"},"canonical_url":"https://search.dev.ndltd.org/etd/east-anglia/oai:ueaeprints.uea.ac.uk:57216","repository":{"repo_id":"east-anglia","name":"University of East Anglia","base_url":"https://ueaeprints.uea.ac.uk/cgi/oai2"},"display":{"title":"Detection of explosive materials","abstract":"Explosive materials are increasingly being exploited by criminal organisations,with harmful, devastating and destructive effects on targeted societies; highlighting the demand for sensitive, reliable and quick explosive detection methods that can be used in a range of environments. Immunoassays are one type of test that can provide these qualities. Immunoassays use antibodies to detect a molecule of interest (analyte). A common type of user-friendly immunoassay that has been employed for a wide-range of applications is the lateral flow immunoassay (LFIA). A LFIA simplifies the immunoassay procedure, applying the principle (discussed in detail later) to perform along a single axis by utilising a porous membrane. This type of test is quick, sensitive and relatively easy to use, which means it is accessible by non-specialised personnel. This research uses a competitive immunoassay format, which arises from competitive binding between a free form and a bound form of the analyte. Successful detection of explosive material: 2,4,6-trinitrotoluene (TNT) has been demonstrated via a competitive immunoassay technique. Through optimisation of reagents and conditions, the limit of detection is 0.03 μg ml-1 (300 pg per fingerprint deposit) on a plate assay format. Initial steps have been taken to apply this mechanism to the LFIA format to produce a test. However, further research is required before a functioning device is ready for commercial value.","abstract_html":"Explosive materials are increasingly being exploited by criminal organisations,with harmful, devastating and destructive effects on targeted societies; highlighting the demand for sensitive, reliable and quick explosive detection methods that can be used in a range of environments. Immunoassays are one type of test that can provide these qualities. Immunoassays use antibodies to detect a molecule of interest (analyte). A common type of user-friendly immunoassay that has been employed for a wide-range of applications is the lateral flow immunoassay (LFIA). A LFIA simplifies the immunoassay procedure, applying the principle (discussed in detail later) to perform along a single axis by utilising a porous membrane. This type of test is quick, sensitive and relatively easy to use, which means it is accessible by non-specialised personnel. This research uses a competitive immunoassay format, which arises from competitive binding between a free form and a bound form of the analyte. Successful detection of explosive material: 2,4,6-trinitrotoluene (TNT) has been demonstrated via a competitive immunoassay technique. Through optimisation of reagents and conditions, the limit of detection is 0.03 μg ml-1 (300 pg per fingerprint deposit) on a plate assay format. Initial steps have been taken to apply this mechanism to the LFIA format to produce a test. 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Immunoassays are one type of test that can provide these qualities. Immunoassays use antibodies to detect a molecule of interest (analyte). A common type of user-friendly immunoassay that has been employed for a wide-range of applications is the lateral flow immunoassay (LFIA). A LFIA simplifies the immunoassay procedure, applying the principle (discussed in detail later) to perform along a single axis by utilising a porous membrane. This type of test is quick, sensitive and relatively easy to use, which means it is accessible by non-specialised personnel. This research uses a competitive immunoassay format, which arises from competitive binding between a free form and a bound form of the analyte. Successful detection of explosive material: 2,4,6-trinitrotoluene (TNT) has been demonstrated via a competitive immunoassay technique. Through optimisation of reagents and conditions, the limit of detection is 0.03 μg ml-1 (300 pg per fingerprint deposit) on a plate assay format. Initial steps have been taken to apply this mechanism to the LFIA format to produce a test. However, further research is required before a functioning device is ready for commercial value."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Detection of explosive materials"]}]}],"canonical_facts":{"dc:creator":["Moore, Brydie"],"dc:date":["2015-09"],"dc:date.issued":["2015-09"],"dc:description.abstract":["Explosive materials are increasingly being exploited by criminal organisations,with harmful, devastating and destructive effects on targeted societies; highlighting the demand for sensitive, reliable and quick explosive detection methods that can be used in a range of environments. Immunoassays are one type of test that can provide these qualities. Immunoassays use antibodies to detect a molecule of interest (analyte). A common type of user-friendly immunoassay that has been employed for a wide-range of applications is the lateral flow immunoassay (LFIA). A LFIA simplifies the immunoassay procedure, applying the principle (discussed in detail later) to perform along a single axis by utilising a porous membrane. This type of test is quick, sensitive and relatively easy to use, which means it is accessible by non-specialised personnel. This research uses a competitive immunoassay format, which arises from competitive binding between a free form and a bound form of the analyte. Successful detection of explosive material: 2,4,6-trinitrotoluene (TNT) has been demonstrated via a competitive immunoassay technique. Through optimisation of reagents and conditions, the limit of detection is 0.03 μg ml-1 (300 pg per fingerprint deposit) on a plate assay format. Initial steps have been taken to apply this mechanism to the LFIA format to produce a test. However, further research is required before a functioning device is ready for commercial value."],"dc:format":["application/pdf"],"dc:identifier.uri":["https://ueaeprints.uea.ac.uk/id/eprint/57216/1/4628659_MSc_by_Research_Thesis.pdf"],"dc:language":["en"],"dc:publisher.department":["School of Chemistry"],"dc:publisher.institution":["University of East Anglia"],"dc:relation.isreferencedby":["https://ueaeprints.uea.ac.uk/id/eprint/57216/"],"dc:title":["Detection of explosive materials"],"dc:type":["Thesis"],"dc:type.qualificationlevel":["masters"],"dc:type.qualificationname":["other"]},"updated_at":"2026-07-24T02:12:13Z"}