{"id":{"repo_id":"east-anglia","oai_identifier":"oai:ueaeprints.uea.ac.uk:56830"},"canonical_url":"https://search.dev.ndltd.org/etd/east-anglia/oai:ueaeprints.uea.ac.uk:56830","repository":{"repo_id":"east-anglia","name":"University of East Anglia","base_url":"https://ueaeprints.uea.ac.uk/cgi/oai2"},"display":{"title":"Structural and spectroscopic studies on the porin-cytochrome complex from Shewanella oneidensis MR-1","abstract":"The\"outer\"membrane,\"hetero!trimeric\"multi!heme\"cytochrome\"complex\"MtrCAB,\"enables\"the\" process\"of\"dissimilatory\"metal\"reduction\"(DMR)\"in\"Shewanella(oneidensis.\" \"\" The\"properties\"of\" the\"decaheme\"protein\"MtrA\"as\"well\"as\"a\" truncated\"version\"of\" this\"protein\" were\" investigated\" using\" analytical\" ultracentrifugation\" (AUC),\" small\" angle\" X!ray\" scattering\" (SAXS)\" and\" spectropotentiometric\" techniques.\" MtrA\" and\" a\" truncated\"N!terminal\" MtrA\" construct\"containing\" the\" five\"N!terminal\"hemes\"(MtrA\"N)\"were\"both\"observed\" to\"be\"prolate\" and\"highly\"extended\" along\"one\" axis.\"Through\" aligning\"MtrA\"N\"with\"MtrA,\" the\"N\" terminus\" of\" the\"MtrA\"structure\"was\"identified.\"\"Redox\"titration\"experiments\"were\"performed\"on\"MtrA\"as\" well\" as\" N\" and\"C\" terminal\" truncations.\"From\" these\" titrations\" three\"distinct\" groups\" of\" hemes\" were\" identified;\" a\" high,\" a\" middle\" potential\" and\" a\" low\" potential\" group\" of\" hemes.\" The\" five\"N\" terminal\"hemes\"contained\"the\"high\"and\"middle\"potential\"groups\"of\"hemes;\"whereas\"the\"five\"C\" terminal\"hemes\"contained\"the\"middle\"and\"low\"potential\"groups\"of\"hemes.\" \" The\"MtrCAB\" complex\"was\" inserted\" into\" liposomes\" containing\" either\"methyl\" viologen,\" small\" tetra\" heme\" cytochrome\" (STC)\" or\" cytochrome\" c.\" Using\" these\" proteoliposomes\" the\" rate\" of\" electron\"transfer\"across\"MtrCAB\"was\"investigated.\"MtrCAB\"was\"seen\"to\"enable\"the\"reduction\" and\"oxidation\"of\"methyl\"viologen\"and\"STC,\"but\"only\"the\"reduction\"of\"cytochrome\"c.\"\"STC\"was\" reduced\" during\" the\" experiments,\" implying\" an\" electron\" storage\" role\" in\" the\" periplasm\" during\"respiration.\" \"Finally,\"the\" structure\" of\" MtrCAB\" was\" investigated\" through\" small\" angle\" neutron\" scattering\" (SANS).\"Structures\"of\"MtrCAB\"were\"highly\"elongated\"with\"a\"globular\"head\" region\"and\"a\" tail\" region.\"Through\"density\"matching\"buffers,\"scattering\"produced\"from\"detergents\"was\"removed\" and\"a\"prediction\"of\"the\"relative\"location\"of\"MtrB\"within\"the\"model\"made.\"The\"model\"produced\" was\" found\" to\" be\" long\" enough\" to\" span\" the\" periplasm\" enabling\" direct\" contact\" of\"MtrA\" with\" proteins\"on\"the\"inner\"membrane.\"\"","abstract_html":"The"outer"membrane,"hetero!trimeric"multi!heme"cytochrome"complex"MtrCAB,"enables"the" process"of"dissimilatory"metal"reduction"(DMR)"in"Shewanella(oneidensis." "" The"properties"of" the"decaheme"protein"MtrA"as"well"as"a" truncated"version"of" this"protein" were" investigated" using" analytical" ultracentrifugation" (AUC)," small" angle" X!ray" scattering" (SAXS)" and" spectropotentiometric" techniques." MtrA" and" a" truncated"N!terminal" MtrA" construct"containing" the" five"N!terminal"hemes"(MtrA"N)"were"both"observed" to"be"prolate" and"highly"extended" along"one" axis."Through" aligning"MtrA"N"with"MtrA," the"N" terminus" of" the"MtrA"structure"was"identified.""Redox"titration"experiments"were"performed"on"MtrA"as" well" as" N" and"C" terminal" truncations."From" these" titrations" three"distinct" groups" of" hemes" were" identified;" a" high," a" middle" potential" and" a" low" potential" group" of" hemes." The" five"N" terminal"hemes"contained"the"high"and"middle"potential"groups"of"hemes;"whereas"the"five"C" terminal"hemes"contained"the"middle"and"low"potential"groups"of"hemes." " The"MtrCAB" complex"was" inserted" into" liposomes" containing" either"methyl" viologen," small" tetra" heme" cytochrome" (STC)" or" cytochrome" c." Using" these" proteoliposomes" the" rate" of" electron"transfer"across"MtrCAB"was"investigated."MtrCAB"was"seen"to"enable"the"reduction" and"oxidation"of"methyl"viologen"and"STC,"but"only"the"reduction"of"cytochrome"c.""STC"was" reduced" during" the" experiments," implying" an" electron" storage" role" in" the" periplasm" during"respiration." "Finally,"the" structure" of" MtrCAB" was" investigated" through" small" angle" neutron" scattering" (SANS)."Structures"of"MtrCAB"were"highly"elongated"with"a"globular"head" region"and"a" tail" region."Through"density"matching"buffers,"scattering"produced"from"detergents"was"removed" and"a"prediction"of"the"relative"location"of"MtrB"within"the"model"made."The"model"produced" was" found" to" be" long" enough" to" span" the" periplasm" enabling" direct" contact" of"MtrA" with" proteins"on"the"inner"membrane.""","abstract_has_math":false,"creators":["Lawes, Matthew"],"institution":"University of East Anglia","degree_name":"phd","degree_level":"doctoral","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-04","date_published":"2015-04","updated_at":"2026-07-24T02:12:10Z","subjects":[],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":null,"outbound_label":null,"outbound_source":null},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Lawes, Matthew"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-04"]},{"key":"dc:date.issued","label":"Date","values":["2015-04"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["School of Biological Sciences"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["University of East Anglia"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://ueaeprints.uea.ac.uk/id/eprint/56830/"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["doctoral"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["phd"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://ueaeprints.uea.ac.uk/id/eprint/56830/1/Matthew_Lawes_Thesis.pdf"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The\"outer\"membrane,\"hetero!trimeric\"multi!heme\"cytochrome\"complex\"MtrCAB,\"enables\"the\" process\"of\"dissimilatory\"metal\"reduction\"(DMR)\"in\"Shewanella(oneidensis.\" \"\" The\"properties\"of\" the\"decaheme\"protein\"MtrA\"as\"well\"as\"a\" truncated\"version\"of\" this\"protein\" were\" investigated\" using\" analytical\" ultracentrifugation\" (AUC),\" small\" angle\" X!ray\" scattering\" (SAXS)\" and\" spectropotentiometric\" techniques.\" MtrA\" and\" a\" truncated\"N!terminal\" MtrA\" construct\"containing\" the\" five\"N!terminal\"hemes\"(MtrA\"N)\"were\"both\"observed\" to\"be\"prolate\" and\"highly\"extended\" along\"one\" axis.\"Through\" aligning\"MtrA\"N\"with\"MtrA,\" the\"N\" terminus\" of\" the\"MtrA\"structure\"was\"identified.\"\"Redox\"titration\"experiments\"were\"performed\"on\"MtrA\"as\" well\" as\" N\" and\"C\" terminal\" truncations.\"From\" these\" titrations\" three\"distinct\" groups\" of\" hemes\" were\" identified;\" a\" high,\" a\" middle\" potential\" and\" a\" low\" potential\" group\" of\" hemes.\" The\" five\"N\" terminal\"hemes\"contained\"the\"high\"and\"middle\"potential\"groups\"of\"hemes;\"whereas\"the\"five\"C\" terminal\"hemes\"contained\"the\"middle\"and\"low\"potential\"groups\"of\"hemes.\" \" The\"MtrCAB\" complex\"was\" inserted\" into\" liposomes\" containing\" either\"methyl\" viologen,\" small\" tetra\" heme\" cytochrome\" (STC)\" or\" cytochrome\" c.\" Using\" these\" proteoliposomes\" the\" rate\" of\" electron\"transfer\"across\"MtrCAB\"was\"investigated.\"MtrCAB\"was\"seen\"to\"enable\"the\"reduction\" and\"oxidation\"of\"methyl\"viologen\"and\"STC,\"but\"only\"the\"reduction\"of\"cytochrome\"c.\"\"STC\"was\" reduced\" during\" the\" experiments,\" implying\" an\" electron\" storage\" role\" in\" the\" periplasm\" during\"respiration.\" \"Finally,\"the\" structure\" of\" MtrCAB\" was\" investigated\" through\" small\" angle\" neutron\" scattering\" (SANS).\"Structures\"of\"MtrCAB\"were\"highly\"elongated\"with\"a\"globular\"head\" region\"and\"a\" tail\" region.\"Through\"density\"matching\"buffers,\"scattering\"produced\"from\"detergents\"was\"removed\" and\"a\"prediction\"of\"the\"relative\"location\"of\"MtrB\"within\"the\"model\"made.\"The\"model\"produced\" was\" found\" to\" be\" long\" enough\" to\" span\" the\" periplasm\" enabling\" direct\" contact\" of\"MtrA\" with\" proteins\"on\"the\"inner\"membrane.\"\""]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Structural and spectroscopic studies on the porin-cytochrome complex from Shewanella oneidensis MR-1"]}]}],"canonical_facts":{"dc:creator":["Lawes, Matthew"],"dc:date":["2015-04"],"dc:date.issued":["2015-04"],"dc:description.abstract":["The\"outer\"membrane,\"hetero!trimeric\"multi!heme\"cytochrome\"complex\"MtrCAB,\"enables\"the\" process\"of\"dissimilatory\"metal\"reduction\"(DMR)\"in\"Shewanella(oneidensis.\" \"\" The\"properties\"of\" the\"decaheme\"protein\"MtrA\"as\"well\"as\"a\" truncated\"version\"of\" this\"protein\" were\" investigated\" using\" analytical\" ultracentrifugation\" (AUC),\" small\" angle\" X!ray\" scattering\" (SAXS)\" and\" spectropotentiometric\" techniques.\" MtrA\" and\" a\" truncated\"N!terminal\" MtrA\" construct\"containing\" the\" five\"N!terminal\"hemes\"(MtrA\"N)\"were\"both\"observed\" to\"be\"prolate\" and\"highly\"extended\" along\"one\" axis.\"Through\" aligning\"MtrA\"N\"with\"MtrA,\" the\"N\" terminus\" of\" the\"MtrA\"structure\"was\"identified.\"\"Redox\"titration\"experiments\"were\"performed\"on\"MtrA\"as\" well\" as\" N\" and\"C\" terminal\" truncations.\"From\" these\" titrations\" three\"distinct\" groups\" of\" hemes\" were\" identified;\" a\" high,\" a\" middle\" potential\" and\" a\" low\" potential\" group\" of\" hemes.\" The\" five\"N\" terminal\"hemes\"contained\"the\"high\"and\"middle\"potential\"groups\"of\"hemes;\"whereas\"the\"five\"C\" terminal\"hemes\"contained\"the\"middle\"and\"low\"potential\"groups\"of\"hemes.\" \" The\"MtrCAB\" complex\"was\" inserted\" into\" liposomes\" containing\" either\"methyl\" viologen,\" small\" tetra\" heme\" cytochrome\" (STC)\" or\" cytochrome\" c.\" Using\" these\" proteoliposomes\" the\" rate\" of\" electron\"transfer\"across\"MtrCAB\"was\"investigated.\"MtrCAB\"was\"seen\"to\"enable\"the\"reduction\" and\"oxidation\"of\"methyl\"viologen\"and\"STC,\"but\"only\"the\"reduction\"of\"cytochrome\"c.\"\"STC\"was\" reduced\" during\" the\" experiments,\" implying\" an\" electron\" storage\" role\" in\" the\" periplasm\" during\"respiration.\" \"Finally,\"the\" structure\" of\" MtrCAB\" was\" investigated\" through\" small\" angle\" neutron\" scattering\" (SANS).\"Structures\"of\"MtrCAB\"were\"highly\"elongated\"with\"a\"globular\"head\" region\"and\"a\" tail\" region.\"Through\"density\"matching\"buffers,\"scattering\"produced\"from\"detergents\"was\"removed\" and\"a\"prediction\"of\"the\"relative\"location\"of\"MtrB\"within\"the\"model\"made.\"The\"model\"produced\" was\" found\" to\" be\" long\" enough\" to\" span\" the\" periplasm\" enabling\" direct\" contact\" of\"MtrA\" with\" proteins\"on\"the\"inner\"membrane.\"\""],"dc:format":["application/pdf"],"dc:identifier.uri":["https://ueaeprints.uea.ac.uk/id/eprint/56830/1/Matthew_Lawes_Thesis.pdf"],"dc:language":["en"],"dc:publisher.department":["School of Biological Sciences"],"dc:publisher.institution":["University of East Anglia"],"dc:relation.isreferencedby":["https://ueaeprints.uea.ac.uk/id/eprint/56830/"],"dc:title":["Structural and spectroscopic studies on the porin-cytochrome complex from Shewanella oneidensis MR-1"],"dc:type":["Thesis"],"dc:type.qualificationlevel":["doctoral"],"dc:type.qualificationname":["phd"]},"updated_at":"2026-07-24T02:12:10Z"}