Microtubule Organisation in Normal and Colon Cancer Cells
type: CreativeWork
description: Abstract+ ApicoNbasal" polarisation" of" epithelial" cells" is" essential" for" normal" function," with" the" rearrangement" of" the"microtubule" cytoskeleton" on" differentiation" critical" for" the" establishment" of" polarity." "During" this" process,"microtubules" reorganise" to" form"a"stabilised"array"of"apicoNbasal"bundles"via"a"release"and"capture"mechanism."" Disruption"of"this"array"can"cause"a"loss"of"epithelial"polarity,"which"can"contribute"to" diseases" such"as" cancer." "Microtubule" rearrangements" are"driven"by"their" inherent" dynamics,"which" are" regulated"by"+TIP"proteins." "This"project" aims" to"examine" the" mechanisms" behind" apicoNbasal" array" formation," and" how" they" may" be" compromised" in" colorectal" cancer." " It" has" focussed" on" two" +TIPs;" APC" (which" is" mutated" in" most" colorectal" cancers)," and" EB2" (which" has" also" been" observed" upregulated"in"cancer)." The"role"of"APC"at"the"cortex"was"examining"using"cell"lines"containing"wild" type"or"APC" truncation"mutations." "Microtubule"orientation" at" junctional" sites"was" determined,"with"Nocodazole"regrowth"assays"used"to"determine"cortical"approach."" Additionally,"the"angle"of"the"mitotic"spindles"was"analysed"in"the"different"cell"lines." The" impact" of" EB2" loss" on" microtubule" organisation" was" determined" by" immunolabelling" following" siRNA" treatment." "A" fluorescently" tagged" EB2" construct" was"created"and"used"to"rescue"depleted"phenotypes.""The"effect"of"EB2"depletion" on"microtubule"stability"was"assessed"by"analysing"live"microtubule"dynamics.""The" impact"of"EB2"overexpression"on"cell"migration"was"assessed"by"creating"a"stable"EB2" overexpressing" cell" line" and" live" imaging" sparsely" seeded" cells" and" scratch" assays."" Overexpressing"cells"were"grown"in"3D"culture"to"study"any"effects"on"polarisation." The" TC7" cell" line" containing" truncated" APC" possessed" fewer" microtubules" orientated"perpendicular"to"the"cortex"than"wildNty…
abstract: Abstract+ ApicoNbasal" polarisation" of" epithelial" cells" is" essential" for" normal" function," with" the" rearrangement" of" the"microtubule" cytoskeleton" on" differentiation" critical" for" the" establishment" of" polarity." "During" this" process,"microtubules" reorganise" to" form"a"stabilised"array"of"apicoNbasal"bundles"via"a"release"and"capture"mechanism."" Disruption"of"this"array"can"cause"a"loss"of"epithelial"polarity,"which"can"contribute"to" diseases" such"as" cancer." "Microtubule" rearrangements" are"driven"by"their" inherent" dynamics,"which" are" regulated"by"+TIP"proteins." "This"project" aims" to"examine" the" mechanisms" behind" apicoNbasal" array" formation," and" how" they" may" be" compromised" in" colorectal" cancer." " It" has" focussed" on" two" +TIPs;" APC" (which" is" mutated" in" most" colorectal" cancers)," and" EB2" (which" has" also" been" observed" upregulated"in"cancer)." The"role"of"APC"at"the"cortex"was"examining"using"cell"lines"containing"wild" type"or"APC" truncation"mutations." "Microtubule"orientation" at" junctional" sites"was" determined,"with"Nocodazole"regrowth"assays"used"to"determine"cortical"approach."" Additionally,"the"angle"of"the"mitotic"spindles"was"analysed"in"the"different"cell"lines." The" impact" of" EB2" loss" on" microtubule" organisation" was" determined" by" immunolabelling" following" siRNA" treatment." "A" fluorescently" tagged" EB2" construct" was"created"and"used"to"rescue"depleted"phenotypes.""The"effect"of"EB2"depletion" on"microtubule"stability"was"assessed"by"analysing"live"microtubule"dynamics.""The" impact"of"EB2"overexpression"on"cell"migration"was"assessed"by"creating"a"stable"EB2" overexpressing" cell" line" and" live" imaging" sparsely" seeded" cells" and" scratch" assays."" Overexpressing"cells"were"grown"in"3D"culture"to"study"any"effects"on"polarisation." The" TC7" cell" line" containing" truncated" APC" possessed" fewer" microtubules" orientated"perpendicular"to"the"cortex"than"wildNty…
author: Gadsby, Jonathan
language: en
date published: 2014-01
date modified: 2026-07-24T02:11:59Z
provider: University of East Anglia
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of East Anglia