Targeted nanoparticle platforms for selective photodynamic therapy of cancer
type: CreativeWork
description: Numerous studies have demonstrated the substantial improvement in the photodynamic therapy (PDT) of cancer using multifunctional nanoparticles. The tumour-selectivity of nanoparticles carrying PDT agents can be achieved through conjugation to cancer-specific biomolecules that provide the particles with an improved tumour localisation and a marked enhancement in the therapeutic outcome. The primary aim of this thesis was to establish the therapeutic potential of lectin, a carbohydrate binding protein, targeted PDT treatment of cancer cells using zinc phthalocyanine (C11Pc)-PEG gold nanoparticles (ca. 4 nm). Using a number of colorimetric techniques the interactions of Concanavalin A and succinyl-Concanavalin A with MCF-7 and SK-BR-3 human breast cancer cells expressing α-mannose was explored. The targeted PDT treatment of the breast cancer cells was attempted using C11Pc-PEG gold nanoparticles conjugated to Concanavalin A. However, Concanavalin A conjugation provided no enhancement in photodynamic breast cancer cell kill, as compared to the non-conjugated C11Pc-PEG gold nanoparticles. The lectin jacalin was also conjugated to C11Pc-PEG gold nanoparticles to target HT-29 human colon cancer cells expressing the oncofoetal Thomsen-Friedenreich (T) antigen. Using laser scanning confocal microscopy a substantial improvement in the cellular internalisation was observed with the jacalin nanoparticle conjugates, as compared to the non-conjugated particles. Similarly, the phototoxicity of the HT-29 cells incubated with the jacalin conjugates was dramatically improved following irradiation at 633 nm, as compared to treatment using the non-conjugated particles. The PDT efficacy of the lectin-photosensitising nanoparticle conjugates was competitively inhibited by methyl-α-galactopyranoside and the T antigen-expressing glycoprotein, asialofetuin. The ApoTox-GloTM triplex assay confirmed that the jacalin targeted phototoxicity of the HT-29 cells was through necrosis, with no evide…
abstract: Numerous studies have demonstrated the substantial improvement in the photodynamic therapy (PDT) of cancer using multifunctional nanoparticles. The tumour-selectivity of nanoparticles carrying PDT agents can be achieved through conjugation to cancer-specific biomolecules that provide the particles with an improved tumour localisation and a marked enhancement in the therapeutic outcome. The primary aim of this thesis was to establish the therapeutic potential of lectin, a carbohydrate binding protein, targeted PDT treatment of cancer cells using zinc phthalocyanine (C11Pc)-PEG gold nanoparticles (ca. 4 nm). Using a number of colorimetric techniques the interactions of Concanavalin A and succinyl-Concanavalin A with MCF-7 and SK-BR-3 human breast cancer cells expressing α-mannose was explored. The targeted PDT treatment of the breast cancer cells was attempted using C11Pc-PEG gold nanoparticles conjugated to Concanavalin A. However, Concanavalin A conjugation provided no enhancement in photodynamic breast cancer cell kill, as compared to the non-conjugated C11Pc-PEG gold nanoparticles. The lectin jacalin was also conjugated to C11Pc-PEG gold nanoparticles to target HT-29 human colon cancer cells expressing the oncofoetal Thomsen-Friedenreich (T) antigen. Using laser scanning confocal microscopy a substantial improvement in the cellular internalisation was observed with the jacalin nanoparticle conjugates, as compared to the non-conjugated particles. Similarly, the phototoxicity of the HT-29 cells incubated with the jacalin conjugates was dramatically improved following irradiation at 633 nm, as compared to treatment using the non-conjugated particles. The PDT efficacy of the lectin-photosensitising nanoparticle conjugates was competitively inhibited by methyl-α-galactopyranoside and the T antigen-expressing glycoprotein, asialofetuin. The ApoTox-GloTM triplex assay confirmed that the jacalin targeted phototoxicity of the HT-29 cells was through necrosis, with no evide…
author: Obaid, G
language: en
date published: 2013-02
date modified: 2026-07-24T02:11:54Z
provider: University of East Anglia
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of East Anglia