{"id":{"repo_id":"dut","oai_identifier":"oai:openscholar.dut.ac.za:10321/307"},"canonical_url":"https://search.dev.ndltd.org/etd/dut/oai:openscholar.dut.ac.za:10321/307","repository":{"repo_id":"dut","name":"Durban University of Technology","base_url":"https://openscholar.dut.ac.za/server/oai/request"},"display":{"title":"Comparative analysis of genetically modified maize by implementation of a half-seed extraction technique","abstract":"Submitted in fulfilment of the requirements for the Degree of Master of Technology: Biotechnology, Durban University of Technology, Durban, South Africa, 2007.","abstract_html":"Submitted in fulfilment of the requirements for the Degree of Master of Technology: Biotechnology, Durban University of Technology, Durban, South Africa, 2007.","abstract_has_math":false,"creators":["Pienaar, Fernando"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Permaul, Kugen"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2008,"date_issued":"2008-07-16T12:46:25Z","date_published":"2008-07-16T12:46:25Z","updated_at":"2026-07-27T19:20:48Z","subjects":["Corn","Crops","Plant genetic engineering","Crop improvement","Biotechnology--Dissertations, Academic","Corn--Genetic engineering","Crops--Genetic engineering","Corn--Biotechnology"],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["308421","https://doi.org/10.51415/10321/307"],"render_values":[{"text":"308421","href":null,"code":true},{"text":"https://doi.org/10.51415/10321/307","href":"https://doi.org/10.51415/10321/307","code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/10321/307","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Permaul, Kugen"]},{"key":"dc:creator","label":"Author","values":["Pienaar, Fernando"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2008-07-16T12:46:25Z","2007"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Corn","Crops","Plant genetic engineering","Crop improvement","Biotechnology--Dissertations, Academic","Corn--Genetic engineering","Crops--Genetic engineering","Corn--Biotechnology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["308421","http://hdl.handle.net/10321/307","https://doi.org/10.51415/10321/307"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Submitted in fulfilment of the requirements for the Degree of Master of Technology: Biotechnology, Durban University of Technology, Durban, South Africa, 2007.","The development of transgenic plants resulted in the need to utilize the various molecular methods (e.g., ELISA, real - time PCR etc.) for the detection or analysis of the presence or absence of a specific trait in a particular plant (Bt in this study). The overall aim of this study was to optimize a half – seed extraction technique as part of a laboratory protocol for transgenic maize plants and to explore the possibility of using the following molecular techniques: horizontal isoelectric focusing, real - time PCR and ELISA, as methods for detection of the Bt trait for incorporation into the half – seed extraction protocol.","M"]},{"key":"dc:format","label":"Dc Format","values":["81 p","application/pdf"]},{"key":"dc:title","label":"Title","values":["Comparative analysis of genetically modified maize by implementation of a half-seed extraction technique"]}]}],"canonical_facts":{"dc:contributor":["Permaul, Kugen"],"dc:creator":["Pienaar, Fernando"],"dc:date":["2008-07-16T12:46:25Z","2007"],"dc:description":["Submitted in fulfilment of the requirements for the Degree of Master of Technology: Biotechnology, Durban University of Technology, Durban, South Africa, 2007.","The development of transgenic plants resulted in the need to utilize the various molecular methods (e.g., ELISA, real - time PCR etc.) for the detection or analysis of the presence or absence of a specific trait in a particular plant (Bt in this study). The overall aim of this study was to optimize a half – seed extraction technique as part of a laboratory protocol for transgenic maize plants and to explore the possibility of using the following molecular techniques: horizontal isoelectric focusing, real - time PCR and ELISA, as methods for detection of the Bt trait for incorporation into the half – seed extraction protocol.","M"],"dc:format":["81 p","application/pdf"],"dc:identifier":["308421","http://hdl.handle.net/10321/307","https://doi.org/10.51415/10321/307"],"dc:language":["en"],"dc:subject":["Corn","Crops","Plant genetic engineering","Crop improvement","Biotechnology--Dissertations, Academic","Corn--Genetic engineering","Crops--Genetic engineering","Corn--Biotechnology"],"dc:title":["Comparative analysis of genetically modified maize by implementation of a half-seed extraction technique"],"dc:type":["Thesis"]},"updated_at":"2026-07-27T19:20:48Z"}