{"id":{"repo_id":"durham","oai_identifier":"oai:etheses.durham.ac.uk:559"},"canonical_url":"https://search.dev.ndltd.org/etd/durham/oai:etheses.durham.ac.uk:559","repository":{"repo_id":"durham","name":"Durham University","base_url":"http://etheses.dur.ac.uk/cgi/oai2"},"display":{"title":"The identification of potential cis- and trans-acting factors in the regulation of DARK INDUCIBLE 3 (DIN3) expression during darkness and chilling in Arabidopsis thaliana","abstract":"Plant responses to environmental stimuli are co-ordinated by a variety of sensing and signalling mechanisms, which bring appropriate internal changes so that plants are able to adapt to a changing environment. It was the aim of this project to investigate the regulation of one gene: DARK INDUCIBLE 3 (DIN3), specifically the cis- and transacting factors. To achieve these aims, the investigative approach centred on gene expression analysis of linker-scan mutation analysis of 50 base-pairs (bp) of the minimal functional promoter of DIN3. To investigate the contribution made by transacting factors, the effects of over-expression of candidate transcription factor genes were analysed. This project determined that in addition to dark-induced expression already described in the literature, the dark-induction of DIN3 expression could be repressed by low temperature. Specific motifs within the crucial 50bp of the DIN3 promoter were found to be necessary for dark-induced expression, which together was hypothesised to constitute a sugar-responsive sequence. No cis-acting regulatory motifs were found to contribute definitely to the cold responsiveness of DIN3. None of the transcription factor genes investigated, were revealed to have a major role in the dark and cold responsiveness of DIN3. The results of this project suggest that there is considerable cross-talk between dark/sugar regulation and low temperature at the cisand trans-acting level.","abstract_html":"Plant responses to environmental stimuli are co-ordinated by a variety of sensing and signalling mechanisms, which bring appropriate internal changes so that plants are able to adapt to a changing environment. It was the aim of this project to investigate the regulation of one gene: DARK INDUCIBLE 3 (DIN3), specifically the cis- and transacting factors. To achieve these aims, the investigative approach centred on gene expression analysis of linker-scan mutation analysis of 50 base-pairs (bp) of the minimal functional promoter of DIN3. To investigate the contribution made by transacting factors, the effects of over-expression of candidate transcription factor genes were analysed. This project determined that in addition to dark-induced expression already described in the literature, the dark-induction of DIN3 expression could be repressed by low temperature. Specific motifs within the crucial 50bp of the DIN3 promoter were found to be necessary for dark-induced expression, which together was hypothesised to constitute a sugar-responsive sequence. No cis-acting regulatory motifs were found to contribute definitely to the cold responsiveness of DIN3. None of the transcription factor genes investigated, were revealed to have a major role in the dark and cold responsiveness of DIN3. The results of this project suggest that there is considerable cross-talk between dark/sugar regulation and low temperature at the cisand trans-acting level.","abstract_has_math":false,"creators":["O'Hara, Liam Edward"],"institution":"Durham University","degree_name":"Masters","degree_level":"masters","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2010,"date_issued":"2010","date_published":"2010","updated_at":"2026-07-24T02:11:12Z","subjects":[],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":null,"outbound_label":null,"outbound_source":null},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["O'Hara, Liam Edward"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2010"]},{"key":"dc:date.issued","label":"Date","values":["2010"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["Biological and Biomedical Sciences, School of"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["Durham University"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://etheses.durham.ac.uk/id/eprint/559/"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["masters"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["Masters"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://etheses.durham.ac.uk/id/eprint/559/1/Thesis_new.pdf"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Plant responses to environmental stimuli are co-ordinated by a variety of sensing and signalling mechanisms, which bring appropriate internal changes so that plants are able to adapt to a changing environment. It was the aim of this project to investigate the regulation of one gene: DARK INDUCIBLE 3 (DIN3), specifically the cis- and transacting factors. To achieve these aims, the investigative approach centred on gene expression analysis of linker-scan mutation analysis of 50 base-pairs (bp) of the minimal functional promoter of DIN3. To investigate the contribution made by transacting factors, the effects of over-expression of candidate transcription factor genes were analysed. This project determined that in addition to dark-induced expression already described in the literature, the dark-induction of DIN3 expression could be repressed by low temperature. Specific motifs within the crucial 50bp of the DIN3 promoter were found to be necessary for dark-induced expression, which together was hypothesised to constitute a sugar-responsive sequence. No cis-acting regulatory motifs were found to contribute definitely to the cold responsiveness of DIN3. None of the transcription factor genes investigated, were revealed to have a major role in the dark and cold responsiveness of DIN3. The results of this project suggest that there is considerable cross-talk between dark/sugar regulation and low temperature at the cisand trans-acting level."]},{"key":"dc:format","label":"Dc Format","values":["text"]},{"key":"dc:title","label":"Title","values":["The identification of potential cis- and trans-acting factors in the regulation of DARK INDUCIBLE 3 (DIN3) expression during darkness and chilling in Arabidopsis thaliana"]}]}],"canonical_facts":{"dc:creator":["O'Hara, Liam Edward"],"dc:date":["2010"],"dc:date.issued":["2010"],"dc:description.abstract":["Plant responses to environmental stimuli are co-ordinated by a variety of sensing and signalling mechanisms, which bring appropriate internal changes so that plants are able to adapt to a changing environment. It was the aim of this project to investigate the regulation of one gene: DARK INDUCIBLE 3 (DIN3), specifically the cis- and transacting factors. To achieve these aims, the investigative approach centred on gene expression analysis of linker-scan mutation analysis of 50 base-pairs (bp) of the minimal functional promoter of DIN3. To investigate the contribution made by transacting factors, the effects of over-expression of candidate transcription factor genes were analysed. This project determined that in addition to dark-induced expression already described in the literature, the dark-induction of DIN3 expression could be repressed by low temperature. Specific motifs within the crucial 50bp of the DIN3 promoter were found to be necessary for dark-induced expression, which together was hypothesised to constitute a sugar-responsive sequence. No cis-acting regulatory motifs were found to contribute definitely to the cold responsiveness of DIN3. None of the transcription factor genes investigated, were revealed to have a major role in the dark and cold responsiveness of DIN3. The results of this project suggest that there is considerable cross-talk between dark/sugar regulation and low temperature at the cisand trans-acting level."],"dc:format":["text"],"dc:identifier.uri":["https://etheses.durham.ac.uk/id/eprint/559/1/Thesis_new.pdf"],"dc:publisher.department":["Biological and Biomedical Sciences, School of"],"dc:publisher.institution":["Durham University"],"dc:relation.isreferencedby":["https://etheses.durham.ac.uk/id/eprint/559/"],"dc:title":["The identification of potential cis- and trans-acting factors in the regulation of DARK INDUCIBLE 3 (DIN3) expression during darkness and chilling in Arabidopsis thaliana"],"dc:type":["Thesis"],"dc:type.qualificationlevel":["masters"],"dc:type.qualificationname":["Masters"]},"updated_at":"2026-07-24T02:11:12Z"}