Characterisation of the sulfotransferases catalysing the sulfation of xenobiotics and steroids in bovine liver
type: CreativeWork
description: Cattle are a very important part of the human food chain. Administration of veterinary drugs and other xenobiotic compounds to cattle can often result in the accumulation of metabolic residues in edible tissues that can potentially affect humans through the food chain. An understanding of drug metabolism in this species is vital to ensure safe use of drugs in cattle and eventually the provision of safer animal derived food products to man. Sulfation catalysed by sulfotransferases (SULTs) is an important phase 2 drug metabolising reaction. It is not only involved in the detoxification of drugs and xenobiotics but also in the bioactivation of procarcinogens. It is also important in the metabolism of several drugs used routinely in cattle. However, very little work has been carried out on SULTs in cattle. A variety of in vitro tools are available to study drug metabolising enzymes (DMEs) like SULTs. These include tissue microsomes, cytosol, recombinant enzymes and isolated cells such as the hepatocytes.Recombinant SULTs are important tools that can be used for the study of isoform specific drug biotransformation, drug-drug interactions and the effect of genetic polymorphisms on the activity of specific isoforms. As the liver is the major drug metabolising organ in the body, it is essential to study expression and activity of cytosolic liver sulfotransferases. Hepatocytes contain DMEs and drug transporters along with all the necessary cofactors that represent in vivo conditions. This makes hepatocytes a better representative of in vivo conditions as compared to microsomes, cytosol or recombinant enzymes. In this study we have characterised sulfotransferases in cytosol, recombinant enzymes and hepatocytes.Antibodies previously raised against human sulfotransferase isoforms were used in the detection of cytosolic bovine sulfotransferases. Probe substrates established for activity with human SULTs were used for assessing the activity of recombinant and cytosolic bovine sul…
abstract: Cattle are a very important part of the human food chain. Administration of veterinary drugs and other xenobiotic compounds to cattle can often result in the accumulation of metabolic residues in edible tissues that can potentially affect humans through the food chain. An understanding of drug metabolism in this species is vital to ensure safe use of drugs in cattle and eventually the provision of safer animal derived food products to man. Sulfation catalysed by sulfotransferases (SULTs) is an important phase 2 drug metabolising reaction. It is not only involved in the detoxification of drugs and xenobiotics but also in the bioactivation of procarcinogens. It is also important in the metabolism of several drugs used routinely in cattle. However, very little work has been carried out on SULTs in cattle. A variety of in vitro tools are available to study drug metabolising enzymes (DMEs) like SULTs. These include tissue microsomes, cytosol, recombinant enzymes and isolated cells such as the hepatocytes.Recombinant SULTs are important tools that can be used for the study of isoform specific drug biotransformation, drug-drug interactions and the effect of genetic polymorphisms on the activity of specific isoforms. As the liver is the major drug metabolising organ in the body, it is essential to study expression and activity of cytosolic liver sulfotransferases. Hepatocytes contain DMEs and drug transporters along with all the necessary cofactors that represent in vivo conditions. This makes hepatocytes a better representative of in vivo conditions as compared to microsomes, cytosol or recombinant enzymes. In this study we have characterised sulfotransferases in cytosol, recombinant enzymes and hepatocytes.Antibodies previously raised against human sulfotransferase isoforms were used in the detection of cytosolic bovine sulfotransferases. Probe substrates established for activity with human SULTs were used for assessing the activity of recombinant and cytosolic bovine sul…
author: Choughule, Kanika
language: eng
date published: 2011
date modified: 2026-07-24T02:07:31Z
provider: University of Dundee
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Dundee