The effect of temperature stress on recombination in barley
type: CreativeWork
description: Plant breeding is ultimately dependent on the process of recombination which is responsible for the generation of different genotypes with new allelic combinations. The control of meiotic recombination is increasingly being considered as a possible means of aiding plant breeding to tackle the challenges that it faces, such as the effect of linkage drag. Plant breeding could benefit from controlling meiotic recombination in many ways, as increasing the crossover (CO) frequency and changing CO distribution would help to break up current linkage blocks, which could facilitate introgression of exotic alleles or potentially release useful genetic variation. Such tools would also facilitate related academic tasks such as genetic mapping and cloning. Given the particular nature of its genome structure, barley is a crop that could particularly profit from the breaking up of established linkage blocks, which potentially constitute a bottleneck in breeding as it is estimated that around 25-30% of genes rarely recombine. The present study was initiated following previous studies that indicated that heat stress could increase and change the distribution of COs in barley. This phenomenon was confirmed in the initial experiments of present work by a long temperature treatment. However, the application of this phenomenon in a practical context was complicated by the need to target the heat stress to the correct meiotic stage of the plants to keep the impact on fertility to a minimum. In order to achieve this, a modular tray growing system has been investigated, which allowed a considerable improvement in the accuracy of the application of targeted temperature treatments. This system was used to apply short heat and cold treatments, which showed an increase and decrease in the frequency of COs in pericentromeric regions respectively. This approach is the first complete demonstration of the utility of the application of temperature in a breeding context, as the temperature effect wa…
abstract: Plant breeding is ultimately dependent on the process of recombination which is responsible for the generation of different genotypes with new allelic combinations. The control of meiotic recombination is increasingly being considered as a possible means of aiding plant breeding to tackle the challenges that it faces, such as the effect of linkage drag. Plant breeding could benefit from controlling meiotic recombination in many ways, as increasing the crossover (CO) frequency and changing CO distribution would help to break up current linkage blocks, which could facilitate introgression of exotic alleles or potentially release useful genetic variation. Such tools would also facilitate related academic tasks such as genetic mapping and cloning. Given the particular nature of its genome structure, barley is a crop that could particularly profit from the breaking up of established linkage blocks, which potentially constitute a bottleneck in breeding as it is estimated that around 25-30% of genes rarely recombine. The present study was initiated following previous studies that indicated that heat stress could increase and change the distribution of COs in barley. This phenomenon was confirmed in the initial experiments of present work by a long temperature treatment. However, the application of this phenomenon in a practical context was complicated by the need to target the heat stress to the correct meiotic stage of the plants to keep the impact on fertility to a minimum. In order to achieve this, a modular tray growing system has been investigated, which allowed a considerable improvement in the accuracy of the application of targeted temperature treatments. This system was used to apply short heat and cold treatments, which showed an increase and decrease in the frequency of COs in pericentromeric regions respectively. This approach is the first complete demonstration of the utility of the application of temperature in a breeding context, as the temperature effect wa…
author: Arrieta Salgado, Mikel
language: eng
date published: 2019
date modified: 2026-07-24T02:08:46Z
provider: University of Dundee
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Dundee