A study of the relationship of the internal endocrine environment to the p53 network, cyclin D1 and replication licensing proteins in breast cancer
type: CreativeWork
description: Aim To assess the change in expression of various tumour markers (p53, mcm2 and cyclin D1) with change in internal endocrine environment in patients with primary breast cancer and to study the expression and prognostic association of cyclin D1 and replication licensing proteins (mcm2 and geminin) in invasive breast cancer. Hypothesis The endocrine environment may be critical in the development and efficacy of treatment in breast cancer. Oestrogen has the potential to modulate the function of p53 network and cell cycle proteins (cyclin D1) in breast cancer, the consequences of which could profoundly affect treatment efficacy. It is proposed that the serum oestrogen level may effect the measurement of protein markers in primary breast cancer. It is hypothesised that the replication licensing protein family, mcm, may be a more consistent marker of proliferation than the conventional immunohistochemical marker, Ki-67, in primary breast cancer. Material and Methods Women with a diagnosis of primary, operable, symptomatic breast cancer were eligible and a total of 99 patients (47 premenopausal and 52 postmenopausal) were included in the study. Of these, 30 pre-menopausal and 33 post-menopausal women had paired samples of breast cancer biopsy and venous blood taken at diagnosis and later at the time of resectional surgery. The samples were collected at two time points to study the change in expression of tumour proteins, if any, with the change in serum oestradiol levels in pre-menopausal women, the postmenopausal women served as the control. The rest of the patients included had tissue samples and blood taken at one time point only i.e. at resectional surgey. Samples comprised formalin fixed breast cancer tissues from both time points for immunohistochemistry and FISH; serum was drawn for 17-? oestradiol, progesterone, FSH, LH. Tumour micro-arrays were prepared from the surgical specimens (corresponding to the second time point) and whole tissue sections were used to stud…
abstract: Aim To assess the change in expression of various tumour markers (p53, mcm2 and cyclin D1) with change in internal endocrine environment in patients with primary breast cancer and to study the expression and prognostic association of cyclin D1 and replication licensing proteins (mcm2 and geminin) in invasive breast cancer. Hypothesis The endocrine environment may be critical in the development and efficacy of treatment in breast cancer. Oestrogen has the potential to modulate the function of p53 network and cell cycle proteins (cyclin D1) in breast cancer, the consequences of which could profoundly affect treatment efficacy. It is proposed that the serum oestrogen level may effect the measurement of protein markers in primary breast cancer. It is hypothesised that the replication licensing protein family, mcm, may be a more consistent marker of proliferation than the conventional immunohistochemical marker, Ki-67, in primary breast cancer. Material and Methods Women with a diagnosis of primary, operable, symptomatic breast cancer were eligible and a total of 99 patients (47 premenopausal and 52 postmenopausal) were included in the study. Of these, 30 pre-menopausal and 33 post-menopausal women had paired samples of breast cancer biopsy and venous blood taken at diagnosis and later at the time of resectional surgery. The samples were collected at two time points to study the change in expression of tumour proteins, if any, with the change in serum oestradiol levels in pre-menopausal women, the postmenopausal women served as the control. The rest of the patients included had tissue samples and blood taken at one time point only i.e. at resectional surgey. Samples comprised formalin fixed breast cancer tissues from both time points for immunohistochemistry and FISH; serum was drawn for 17-? oestradiol, progesterone, FSH, LH. Tumour micro-arrays were prepared from the surgical specimens (corresponding to the second time point) and whole tissue sections were used to stud…
author: Roy, Pankaj Gupta
language: eng
date published: 2011
date modified: 2026-07-24T02:07:31Z
provider: University of Dundee
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Dundee