In vitro diagnostic assays (IVDs) play a crucial role in modern biomedical research and clinical decision-making. The overall performance and cost of IVDs depends considerably on the quality of the assay surface being used and the steps/reagents required to complete the assay. Synthetic “bottlebrush” polymers have garnered considerable attention as molecularly defined and tunable biointerfacial coatings for a diverse set of biomedical applications; in particular, their bio-inertness, ability to eliminate nonspecific binding events and stabilize biorecognition elements make them attractive candidates as biosensing surfaces. This dissertation describes the use of “nonfouling” (protein- and cell-resistant) nanoscale polymer bottlebrush films derived from poly(oligo(ethylene glycol) methyl ether methacrylate) (POEGMA) for IVD applications. POEGMA coatings are grown from planar surfaces by surface-initiated atom transfer radical polymerization (SI-ATRP), and then undergo biofunctional modification with biorecognition elements to target specific analytes of interest. This strategy is used to assemble low-cost, “point of care” diagnostic tests which exhibit performance comparable to those used in central laboratories. Further, molecular modification of bottlebrush architecture is used as a controllable design parameter for fine-tuning the physiochemical properties and biological behavior of these surfaces. Taken together, these studies highlight the potential for using POEGMA bottlebrush films as biointerfacial coatings for next-generation IVDs and medical devices.
","abstract_html":"<p>In vitro diagnostic assays (IVDs) play a crucial role in modern biomedical research and clinical decision-making. The overall performance and cost of IVDs depends considerably on the quality of the assay surface being used and the steps/reagents required to complete the assay. Synthetic “bottlebrush” polymers have garnered considerable attention as molecularly defined and tunable biointerfacial coatings for a diverse set of biomedical applications; in particular, their bio-inertness, ability to eliminate nonspecific binding events and stabilize biorecognition elements make them attractive candidates as biosensing surfaces. This dissertation describes the use of “nonfouling” (protein- and cell-resistant) nanoscale polymer bottlebrush films derived from poly(oligo(ethylene glycol) methyl ether methacrylate) (POEGMA) for IVD applications. POEGMA coatings are grown from planar surfaces by surface-initiated atom transfer radical polymerization (SI-ATRP), and then undergo biofunctional modification with biorecognition elements to target specific analytes of interest. This strategy is used to assemble low-cost, “point of care” diagnostic tests which exhibit performance comparable to those used in central laboratories. Further, molecular modification of bottlebrush architecture is used as a controllable design parameter for fine-tuning the physiochemical properties and biological behavior of these surfaces. Taken together, these studies highlight the potential for using POEGMA bottlebrush films as biointerfacial coatings for next-generation IVDs and medical devices.</p>","abstract_has_math":false,"creators":["Joh, Daniel"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Chilkoti, Ashutosh"],"committee_chairs":[],"committee_members":[],"year":2019,"date_issued":"2019","date_published":"2019","updated_at":"2026-07-24T02:07:08Z","subjects":["Bioengineering"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/10161/18642","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Chilkoti, Ashutosh"]},{"key":"dc:creator","label":"Author","values":["Joh, Daniel"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2019-06-07T19:47:54Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2021-05-21T08:17:11Z"]},{"key":"dc:date.issued","label":"Date","values":["2019"]},{"key":"dc:type","label":"Dc Type","values":["Dissertation"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Bioengineering"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://hdl.handle.net/10161/18642"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["In vitro diagnostic assays (IVDs) play a crucial role in modern biomedical research and clinical decision-making. The overall performance and cost of IVDs depends considerably on the quality of the assay surface being used and the steps/reagents required to complete the assay. Synthetic “bottlebrush” polymers have garnered considerable attention as molecularly defined and tunable biointerfacial coatings for a diverse set of biomedical applications; in particular, their bio-inertness, ability to eliminate nonspecific binding events and stabilize biorecognition elements make them attractive candidates as biosensing surfaces. This dissertation describes the use of “nonfouling” (protein- and cell-resistant) nanoscale polymer bottlebrush films derived from poly(oligo(ethylene glycol) methyl ether methacrylate) (POEGMA) for IVD applications. POEGMA coatings are grown from planar surfaces by surface-initiated atom transfer radical polymerization (SI-ATRP), and then undergo biofunctional modification with biorecognition elements to target specific analytes of interest. This strategy is used to assemble low-cost, “point of care” diagnostic tests which exhibit performance comparable to those used in central laboratories. Further, molecular modification of bottlebrush architecture is used as a controllable design parameter for fine-tuning the physiochemical properties and biological behavior of these surfaces. Taken together, these studies highlight the potential for using POEGMA bottlebrush films as biointerfacial coatings for next-generation IVDs and medical devices.
"]},{"key":"dc:title","label":"Title","values":["Molecular and Biofunctional Modification of Conformal POEGMA Bottlebrush Coatings and Applications Toward In Vitro Diagnostics"]}]}],"canonical_facts":{"dc:contributor.advisor":["Chilkoti, Ashutosh"],"dc:creator":["Joh, Daniel"],"dc:date.accessioned":["2019-06-07T19:47:54Z"],"dc:date.available":["2021-05-21T08:17:11Z"],"dc:date.issued":["2019"],"dc:description.abstract":["In vitro diagnostic assays (IVDs) play a crucial role in modern biomedical research and clinical decision-making. The overall performance and cost of IVDs depends considerably on the quality of the assay surface being used and the steps/reagents required to complete the assay. Synthetic “bottlebrush” polymers have garnered considerable attention as molecularly defined and tunable biointerfacial coatings for a diverse set of biomedical applications; in particular, their bio-inertness, ability to eliminate nonspecific binding events and stabilize biorecognition elements make them attractive candidates as biosensing surfaces. This dissertation describes the use of “nonfouling” (protein- and cell-resistant) nanoscale polymer bottlebrush films derived from poly(oligo(ethylene glycol) methyl ether methacrylate) (POEGMA) for IVD applications. POEGMA coatings are grown from planar surfaces by surface-initiated atom transfer radical polymerization (SI-ATRP), and then undergo biofunctional modification with biorecognition elements to target specific analytes of interest. This strategy is used to assemble low-cost, “point of care” diagnostic tests which exhibit performance comparable to those used in central laboratories. Further, molecular modification of bottlebrush architecture is used as a controllable design parameter for fine-tuning the physiochemical properties and biological behavior of these surfaces. Taken together, these studies highlight the potential for using POEGMA bottlebrush films as biointerfacial coatings for next-generation IVDs and medical devices.
"],"dc:identifier.uri":["https://hdl.handle.net/10161/18642"],"dc:subject":["Bioengineering"],"dc:title":["Molecular and Biofunctional Modification of Conformal POEGMA Bottlebrush Coatings and Applications Toward In Vitro Diagnostics"],"dc:type":["Dissertation"]},"updated_at":"2026-07-24T02:07:08Z"}