{"id":{"repo_id":"duke","oai_identifier":"oai:dukespace.lib.duke.edu:10161/14557"},"canonical_url":"https://search.dev.ndltd.org/etd/duke/oai:dukespace.lib.duke.edu:10161/14557","repository":{"repo_id":"duke","name":"Duke University","base_url":"https://dukespace.lib.duke.edu/server/oai/request"},"display":{"title":"Structural and Functional Studies of Nucleoside Transporters","abstract":"<p>Nucleoside transport is required for the salvage pathway of DNA and RNA, provides a transport route for nucleoside-like drugs and regulates adenosine signaling. The eukaryotic cell possesses two evolutionarily unrelated protein families of nucleoside transporters, the concentrative nucleoside transporters and the equilibrative nucleoside transporters. We aimed to elucidate their transport mechanisms using X-ray crystallography and biochemical assays. We solved multiple crystal structures of CNT along its transport cycle at 3.45-4.2 Å, which provide novel insights into the elevator-type transport mechanism of secondary active transporters.</p>","abstract_html":"&lt;p&gt;Nucleoside transport is required for the salvage pathway of DNA and RNA, provides a transport route for nucleoside-like drugs and regulates adenosine signaling. The eukaryotic cell possesses two evolutionarily unrelated protein families of nucleoside transporters, the concentrative nucleoside transporters and the equilibrative nucleoside transporters. We aimed to elucidate their transport mechanisms using X-ray crystallography and biochemical assays. We solved multiple crystal structures of CNT along its transport cycle at 3.45-4.2 Å, which provide novel insights into the elevator-type transport mechanism of secondary active transporters.&lt;/p&gt;","abstract_has_math":false,"creators":["Hirschi, Marsha Marianne"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Lee, Seok-Yong"],"committee_chairs":[],"committee_members":[],"year":2017,"date_issued":"2017","date_published":"2017","updated_at":"2026-07-24T02:07:19Z","subjects":["Biochemistry","Biophysics","facilitative transporter","ion channel","secondary active transporter","transient receptor potential"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/10161/14557","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Lee, Seok-Yong"]},{"key":"dc:creator","label":"Author","values":["Hirschi, Marsha Marianne"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2017-05-16T17:29:01Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2019-04-21T08:17:09Z"]},{"key":"dc:date.issued","label":"Date","values":["2017"]},{"key":"dc:type","label":"Dc Type","values":["Dissertation"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biochemistry","Biophysics","facilitative transporter","ion channel","secondary active transporter","transient receptor potential"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://hdl.handle.net/10161/14557"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["<p>Nucleoside transport is required for the salvage pathway of DNA and RNA, provides a transport route for nucleoside-like drugs and regulates adenosine signaling. The eukaryotic cell possesses two evolutionarily unrelated protein families of nucleoside transporters, the concentrative nucleoside transporters and the equilibrative nucleoside transporters. We aimed to elucidate their transport mechanisms using X-ray crystallography and biochemical assays. We solved multiple crystal structures of CNT along its transport cycle at 3.45-4.2 Å, which provide novel insights into the elevator-type transport mechanism of secondary active transporters.</p>"]},{"key":"dc:title","label":"Title","values":["Structural and Functional Studies of Nucleoside Transporters"]}]}],"canonical_facts":{"dc:contributor.advisor":["Lee, Seok-Yong"],"dc:creator":["Hirschi, Marsha Marianne"],"dc:date.accessioned":["2017-05-16T17:29:01Z"],"dc:date.available":["2019-04-21T08:17:09Z"],"dc:date.issued":["2017"],"dc:description.abstract":["<p>Nucleoside transport is required for the salvage pathway of DNA and RNA, provides a transport route for nucleoside-like drugs and regulates adenosine signaling. The eukaryotic cell possesses two evolutionarily unrelated protein families of nucleoside transporters, the concentrative nucleoside transporters and the equilibrative nucleoside transporters. We aimed to elucidate their transport mechanisms using X-ray crystallography and biochemical assays. We solved multiple crystal structures of CNT along its transport cycle at 3.45-4.2 Å, which provide novel insights into the elevator-type transport mechanism of secondary active transporters.</p>"],"dc:identifier.uri":["https://hdl.handle.net/10161/14557"],"dc:subject":["Biochemistry","Biophysics","facilitative transporter","ion channel","secondary active transporter","transient receptor potential"],"dc:title":["Structural and Functional Studies of Nucleoside Transporters"],"dc:type":["Dissertation"]},"updated_at":"2026-07-24T02:07:19Z"}