{"id":{"repo_id":"drake","oai_identifier":"oai:escholarshare.drake.edu:2092/1424"},"canonical_url":"https://search.dev.ndltd.org/etd/drake/oai:escholarshare.drake.edu:2092/1424","repository":{"repo_id":"drake","name":"Drake University","base_url":"https://escholarshare.drake.edu/server/oai/request"},"display":{"title":"Inhibitory Effects of Ketoconazole on the Oxidation of Linoleic Acid Micelles, Phospholipid Liposomes, and Human Low Density Lipoprotein (h-LDL)","abstract":"Soybean lipoxygenase (SLO) (500-5000 units/ml), Fe(III)Cl3/ascorbate (5-100microM) and hematin (5-100microM) induced oxidative modification of lipids present in micelles of linoleic acid (0.5 mM), liposomes prepared from phospholipid extracts of bovine brain (2.5 mg/ml), and h-LDL (0.5 mg/ml). Oxidation of the lipid substrates was determined by the formation of thiobarbituric acid reactive substances and conjugated dienes. Free iron (Fe(III)Cl3/ascorbate) was the strongest initiator, followed by hematin and SLO. Ketoconazole (KC) attenuated lipid oxidation in a concentration dependent manner at pharmacologically relevant levels (25-100microM) in all three substrates. Pretreatment of liposomes and h-LDL with KC produced greater attenuation of lipid oxidation by the various initiators in comparison to free KC.","abstract_html":"Soybean lipoxygenase (SLO) (500-5000 units/ml), Fe(III)Cl3/ascorbate (5-100microM) and hematin (5-100microM) induced oxidative modification of lipids present in micelles of linoleic acid (0.5 mM), liposomes prepared from phospholipid extracts of bovine brain (2.5 mg/ml), and h-LDL (0.5 mg/ml). Oxidation of the lipid substrates was determined by the formation of thiobarbituric acid reactive substances and conjugated dienes. Free iron (Fe(III)Cl3/ascorbate) was the strongest initiator, followed by hematin and SLO. Ketoconazole (KC) attenuated lipid oxidation in a concentration dependent manner at pharmacologically relevant levels (25-100microM) in all three substrates. Pretreatment of liposomes and h-LDL with KC produced greater attenuation of lipid oxidation by the various initiators in comparison to free KC.","abstract_has_math":false,"creators":["Munroe, Melissa E."],"institution":"Drake University","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1993,"date_issued":"1993-07","date_published":"1993-07","updated_at":"2026-07-27T19:18:32Z","subjects":["Ketoconazole","Low density lipoproteins","Micelles","Liposomes"],"languages":["en_US"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["1993 .M926"],"render_values":[{"text":"1993 .M926","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2092/1424","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Munroe, Melissa E."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2010-09-15T15:13:01Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2010-09-15T15:13:01Z"]},{"key":"dc:date.issued","label":"Date","values":["1993-07"]},{"key":"dc:publisher","label":"Institution","values":["Drake University"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Ketoconazole","Low density lipoproteins","Micelles","Liposomes"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en_US"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["1993 .M926"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/2092/1424"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["36 leaves"]},{"key":"dc:description.abstract","label":"Abstract","values":["Soybean lipoxygenase (SLO) (500-5000 units/ml), Fe(III)Cl3/ascorbate (5-100microM) and hematin (5-100microM) induced oxidative modification of lipids present in micelles of linoleic acid (0.5 mM), liposomes prepared from phospholipid extracts of bovine brain (2.5 mg/ml), and h-LDL (0.5 mg/ml). Oxidation of the lipid substrates was determined by the formation of thiobarbituric acid reactive substances and conjugated dienes. Free iron (Fe(III)Cl3/ascorbate) was the strongest initiator, followed by hematin and SLO. Ketoconazole (KC) attenuated lipid oxidation in a concentration dependent manner at pharmacologically relevant levels (25-100microM) in all three substrates. Pretreatment of liposomes and h-LDL with KC produced greater attenuation of lipid oxidation by the various initiators in comparison to free KC."]},{"key":"dc:title","label":"Title","values":["Inhibitory Effects of Ketoconazole on the Oxidation of Linoleic Acid Micelles, Phospholipid Liposomes, and Human Low Density Lipoprotein (h-LDL)"]}]}],"canonical_facts":{"dc:creator":["Munroe, Melissa E."],"dc:date.accessioned":["2010-09-15T15:13:01Z"],"dc:date.available":["2010-09-15T15:13:01Z"],"dc:date.issued":["1993-07"],"dc:description":["36 leaves"],"dc:description.abstract":["Soybean lipoxygenase (SLO) (500-5000 units/ml), Fe(III)Cl3/ascorbate (5-100microM) and hematin (5-100microM) induced oxidative modification of lipids present in micelles of linoleic acid (0.5 mM), liposomes prepared from phospholipid extracts of bovine brain (2.5 mg/ml), and h-LDL (0.5 mg/ml). Oxidation of the lipid substrates was determined by the formation of thiobarbituric acid reactive substances and conjugated dienes. Free iron (Fe(III)Cl3/ascorbate) was the strongest initiator, followed by hematin and SLO. Ketoconazole (KC) attenuated lipid oxidation in a concentration dependent manner at pharmacologically relevant levels (25-100microM) in all three substrates. Pretreatment of liposomes and h-LDL with KC produced greater attenuation of lipid oxidation by the various initiators in comparison to free KC."],"dc:identifier.other":["1993 .M926"],"dc:identifier.uri":["http://hdl.handle.net/2092/1424"],"dc:language.iso":["en_US"],"dc:publisher":["Drake University"],"dc:subject":["Ketoconazole","Low density lipoproteins","Micelles","Liposomes"],"dc:title":["Inhibitory Effects of Ketoconazole on the Oxidation of Linoleic Acid Micelles, Phospholipid Liposomes, and Human Low Density Lipoprotein (h-LDL)"],"dc:type":["Thesis"]},"updated_at":"2026-07-27T19:18:32Z"}