{"id":{"repo_id":"dialnet","oai_identifier":"oai:dialnet.unirioja.es:TES0000023223"},"canonical_url":"https://search.dev.ndltd.org/etd/dialnet/oai:dialnet.unirioja.es:TES0000023223","repository":{"repo_id":"dialnet","name":"Dialnet","base_url":"https://dialnet.unirioja.es/oaites/OAIHandler"},"display":{"title":"Impact assessment of different agricultural practices in grapevine soil and must microbiota","abstract":"Viticulture, an agricultural activity of great economic, social and cultural relevance, currently faces challenges that compromise its viability, such as climate change, soil degradation, biodiversity loss and the environmental impact of conventional practices. In this context, the microbiota within the viticultural ecosystem plays a critical role in crop health and wine quality. Thanks to next-generation sequencing (NGS) technologies, it is now possible to characterize the composition and functionality of these microbial communities. However, most studies on emerging practices in viticulture have not incorporated a microbiological perspective, limiting our understanding of their real impact on the agroecosystem. In this thesis, we hypothesized that agricultural practices significantly influence the structure and functionality of vineyard microbial communities. The main objective of the thesis was to assess the impact of emerging vineyard management strategies on microbial ecosystems. Specifically, the study aimed to: i) evaluate the effect of organic mulches on the fungal and bacterial communities of soil and must, ii) characterize the impact of five rootstocks under irrigation and drought conditions on the rhizosphere bacterial community and iii) determine how irrigation with ozonated water influences soil bacterial and fungal populations. Three field studies were conducted between 2019 and 2024 in vineyards within the Rioja Qualified Designation of Origin (DOCa) in three representative locations — Logroño, Aldeanueva de Ebro, and Arenzana de Abajo — selected for their edaphoclimatic conditions and vineyard management practices. The three management practices included: i) application of organic mulches (straw, grapevine pruning debris and spent mushroom post), compared with conventional treatments (herbicide and tillage); ii) use of five rootstocks under irrigation and water stress conditions; and iii) irrigation with ozonized water. A total of 246 samples from soil, rhizosphere and must were collected. Total DNA was extracted and analysed using NGS technologies: metabarcoding (16S rRNA and ITS2) for taxonomic profiling, and shotgun metagenomics for functional analysis. The results showed that vintage and location were the primary factors influencing both bacterial and fungal microbiota in soil and must. The evaluated agricultural practices had a smaller, though detectable, impact —primarily in soil and rhizosphere, rather than in must— and their effects varied depending on the treatment type and environmental context. In the organic mulch experiment, significant changes in bacterial beta diversity and fungal alpha diversity were observed in the third year, indicating delayed but notable long-term effects of these practices. However, no relevant changes in the abundance of pathogenic or beneficial fungal taxa were detected. Additionally, there was limited overlap between soil and must microbiota, except for some fermentative fungal taxa, which challenge the hypothesis of microbial migration from soil to must. In the rootstock trial, irrigation emerged as the main modulating factor on rhizosphere bacterial communities. However, under drought conditions, genotype-specific differences emerged, highlighting the role of rootstock in stress scenarios. Key microbial taxa and functions associated with drought were also identified, which may be of great interest in future research. Finally, irrigation with ozonated water did not affect soil bacterial and fungal microbiota, including beneficial bacterial and fungal groups (PGPB) and pathogens (GTD), which raises questions about the potential of ozone as a sustainable irrigation strategy. In conclusion, the impact of agricultural practices on vineyard microbiota is variable and context-dependent, often requiring multiple years to become evident. The effects observed also differ between microbial groups, with bacteria and fungi responding differently. This thesis underscores the importance of evaluating emerging practices over multiple years and under specific conditions to better understand and optimize viticultural management through a microbiological lens.","abstract_html":"Viticulture, an agricultural activity of great economic, social and cultural relevance, currently faces challenges that compromise its viability, such as climate change, soil degradation, biodiversity loss and the environmental impact of conventional practices. In this context, the microbiota within the viticultural ecosystem plays a critical role in crop health and wine quality. Thanks to next-generation sequencing (NGS) technologies, it is now possible to characterize the composition and functionality of these microbial communities. However, most studies on emerging practices in viticulture have not incorporated a microbiological perspective, limiting our understanding of their real impact on the agroecosystem. In this thesis, we hypothesized that agricultural practices significantly influence the structure and functionality of vineyard microbial communities. The main objective of the thesis was to assess the impact of emerging vineyard management strategies on microbial ecosystems. Specifically, the study aimed to: i) evaluate the effect of organic mulches on the fungal and bacterial communities of soil and must, ii) characterize the impact of five rootstocks under irrigation and drought conditions on the rhizosphere bacterial community and iii) determine how irrigation with ozonated water influences soil bacterial and fungal populations. Three field studies were conducted between 2019 and 2024 in vineyards within the Rioja Qualified Designation of Origin (DOCa) in three representative locations — Logroño, Aldeanueva de Ebro, and Arenzana de Abajo — selected for their edaphoclimatic conditions and vineyard management practices. The three management practices included: i) application of organic mulches (straw, grapevine pruning debris and spent mushroom post), compared with conventional treatments (herbicide and tillage); ii) use of five rootstocks under irrigation and water stress conditions; and iii) irrigation with ozonized water. A total of 246 samples from soil, rhizosphere and must were collected. Total DNA was extracted and analysed using NGS technologies: metabarcoding (16S rRNA and ITS2) for taxonomic profiling, and shotgun metagenomics for functional analysis. The results showed that vintage and location were the primary factors influencing both bacterial and fungal microbiota in soil and must. The evaluated agricultural practices had a smaller, though detectable, impact —primarily in soil and rhizosphere, rather than in must— and their effects varied depending on the treatment type and environmental context. In the organic mulch experiment, significant changes in bacterial beta diversity and fungal alpha diversity were observed in the third year, indicating delayed but notable long-term effects of these practices. However, no relevant changes in the abundance of pathogenic or beneficial fungal taxa were detected. Additionally, there was limited overlap between soil and must microbiota, except for some fermentative fungal taxa, which challenge the hypothesis of microbial migration from soil to must. In the rootstock trial, irrigation emerged as the main modulating factor on rhizosphere bacterial communities. However, under drought conditions, genotype-specific differences emerged, highlighting the role of rootstock in stress scenarios. Key microbial taxa and functions associated with drought were also identified, which may be of great interest in future research. Finally, irrigation with ozonated water did not affect soil bacterial and fungal microbiota, including beneficial bacterial and fungal groups (PGPB) and pathogens (GTD), which raises questions about the potential of ozone as a sustainable irrigation strategy. In conclusion, the impact of agricultural practices on vineyard microbiota is variable and context-dependent, often requiring multiple years to become evident. The effects observed also differ between microbial groups, with bacteria and fungi responding differently. This thesis underscores the importance of evaluating emerging practices over multiple years and under specific conditions to better understand and optimize viticultural management through a microbiological lens.","abstract_has_math":false,"creators":["Labarga Varona, David"],"institution":"Universidad de La Rioja (España)","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Pou Mir, Alicia (Universidad de La Rioja)"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2026,"date_issued":"2026","date_published":"2026","updated_at":"2026-07-24T06:27:11Z","subjects":[],"languages":["eng"],"rights":["LICENCIA DE USO: Los documentos a texto completo incluidos en Dialnet son de acceso libre y propiedad de sus autores y/o editores. Por tanto, cualquier acto de reproducción, distribución, comunicación pública y/o transformación total o parcial requiere el consentimiento expreso y escrito de aquéllos. Cualquier enlace al texto completo de estos documentos deberá hacerse a través de la URL oficial de éstos en Dialnet. Más información: https://dialnet.unirioja.es/info/derechosOAI | INTELLECTUAL PROPERTY RIGHTS STATEMENT: Full text documents hosted by Dialnet are protected by copyright and/or related rights. This digital object is accessible without charge, but its use is subject to the licensing conditions set by its authors or editors. Unless expressly stated otherwise in the licensing conditions, you are free to linking, browsing, printing and making a copy for your own personal purposes. All other acts of reproduction and communication to the public are subject to the licensing conditions expressed by editors and authors and require consent from them. Any link to this document should be made using its official URL in Dialnet. More info: https://dialnet.unirioja.es/info/derechosOAI"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://dialnet.unirioja.es/servlet/oaites?codigo=398291","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Pou Mir, Alicia (Universidad de La Rioja)"]},{"key":"dc:creator","label":"Author","values":["Labarga Varona, David"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2026"]},{"key":"dc:publisher","label":"Institution","values":["Universidad de La Rioja (España)"]},{"key":"dc:type","label":"Dc Type","values":["text (thesis)"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["LICENCIA DE USO: Los documentos a texto completo incluidos en Dialnet son de acceso libre y propiedad de sus autores y/o editores. Por tanto, cualquier acto de reproducción, distribución, comunicación pública y/o transformación total o parcial requiere el consentimiento expreso y escrito de aquéllos. Cualquier enlace al texto completo de estos documentos deberá hacerse a través de la URL oficial de éstos en Dialnet. Más información: https://dialnet.unirioja.es/info/derechosOAI | INTELLECTUAL PROPERTY RIGHTS STATEMENT: Full text documents hosted by Dialnet are protected by copyright and/or related rights. This digital object is accessible without charge, but its use is subject to the licensing conditions set by its authors or editors. Unless expressly stated otherwise in the licensing conditions, you are free to linking, browsing, printing and making a copy for your own personal purposes. All other acts of reproduction and communication to the public are subject to the licensing conditions expressed by editors and authors and require consent from them. Any link to this document should be made using its official URL in Dialnet. More info: https://dialnet.unirioja.es/info/derechosOAI"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://dialnet.unirioja.es/servlet/oaites?codigo=398291"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Viticulture, an agricultural activity of great economic, social and cultural relevance, currently faces challenges that compromise its viability, such as climate change, soil degradation, biodiversity loss and the environmental impact of conventional practices. In this context, the microbiota within the viticultural ecosystem plays a critical role in crop health and wine quality. Thanks to next-generation sequencing (NGS) technologies, it is now possible to characterize the composition and functionality of these microbial communities. However, most studies on emerging practices in viticulture have not incorporated a microbiological perspective, limiting our understanding of their real impact on the agroecosystem. In this thesis, we hypothesized that agricultural practices significantly influence the structure and functionality of vineyard microbial communities. The main objective of the thesis was to assess the impact of emerging vineyard management strategies on microbial ecosystems. Specifically, the study aimed to: i) evaluate the effect of organic mulches on the fungal and bacterial communities of soil and must, ii) characterize the impact of five rootstocks under irrigation and drought conditions on the rhizosphere bacterial community and iii) determine how irrigation with ozonated water influences soil bacterial and fungal populations. Three field studies were conducted between 2019 and 2024 in vineyards within the Rioja Qualified Designation of Origin (DOCa) in three representative locations — Logroño, Aldeanueva de Ebro, and Arenzana de Abajo — selected for their edaphoclimatic conditions and vineyard management practices. The three management practices included: i) application of organic mulches (straw, grapevine pruning debris and spent mushroom post), compared with conventional treatments (herbicide and tillage); ii) use of five rootstocks under irrigation and water stress conditions; and iii) irrigation with ozonized water. A total of 246 samples from soil, rhizosphere and must were collected. Total DNA was extracted and analysed using NGS technologies: metabarcoding (16S rRNA and ITS2) for taxonomic profiling, and shotgun metagenomics for functional analysis. The results showed that vintage and location were the primary factors influencing both bacterial and fungal microbiota in soil and must. The evaluated agricultural practices had a smaller, though detectable, impact —primarily in soil and rhizosphere, rather than in must— and their effects varied depending on the treatment type and environmental context. In the organic mulch experiment, significant changes in bacterial beta diversity and fungal alpha diversity were observed in the third year, indicating delayed but notable long-term effects of these practices. However, no relevant changes in the abundance of pathogenic or beneficial fungal taxa were detected. Additionally, there was limited overlap between soil and must microbiota, except for some fermentative fungal taxa, which challenge the hypothesis of microbial migration from soil to must. In the rootstock trial, irrigation emerged as the main modulating factor on rhizosphere bacterial communities. However, under drought conditions, genotype-specific differences emerged, highlighting the role of rootstock in stress scenarios. Key microbial taxa and functions associated with drought were also identified, which may be of great interest in future research. Finally, irrigation with ozonated water did not affect soil bacterial and fungal microbiota, including beneficial bacterial and fungal groups (PGPB) and pathogens (GTD), which raises questions about the potential of ozone as a sustainable irrigation strategy. In conclusion, the impact of agricultural practices on vineyard microbiota is variable and context-dependent, often requiring multiple years to become evident. The effects observed also differ between microbial groups, with bacteria and fungi responding differently. This thesis underscores the importance of evaluating emerging practices over multiple years and under specific conditions to better understand and optimize viticultural management through a microbiological lens.","La viticultura, una actividad agrícola de gran relevancia económica, social y cultural, enfrenta actualmente desafíos que comprometen su viabilidad, como el cambio climático, la degradación del suelo, la pérdida de biodiversidad y el impacto ambiental de las prácticas convencionales. En este contexto, la microbiota dentro del ecosistema vitícola desempeña un papel crítico en la salud del cultivo y la calidad del vino. Gracias a las tecnologías de secuenciación de nueva generación (NGS), ahora es posible caracterizar la composición y funcionalidad de estas comunidades microbianas. Sin embargo, la mayoría de los estudios sobre prácticas emergentes en viticultura no han incorporado una perspectiva microbiológica, lo que limita nuestra comprensión de su impacto real en el agroecosistema. En esta tesis, se planteó la hipótesis de que las prácticas agrícolas influyen significativamente en la estructura y funcionalidad de las comunidades microbianas del viñedo. El objetivo principal de la tesis fue evaluar el impacto de estrategias emergentes de manejo del viñedo sobre los ecosistemas microbianos. Específicamente, el estudio se propuso: i) evaluar el efecto de acolchados orgánicos sobre las comunidades fúngicas y bacterianas del suelo y el mosto, ii) caracterizar el impacto de cinco portainjertos bajo condiciones de riego y sequía sobre la comunidad bacteriana de la rizosfera, y iii) determinar cómo influye el riego con agua ozonizada en las poblaciones bacterianas y fúngicas del suelo. Se realizaron tres estudios de campo entre 2019 y 2024 en viñedos dentro de la Denominación de Origen Calificada (DOCa) Rioja, en tres localidades representativas —Logroño, Aldeanueva de Ebro y Arenzana de Abajo— seleccionadas por sus condiciones edafoclimáticas y prácticas de manejo del viñedo. Las tres prácticas de manejo incluyeron: i) aplicación de acolchados orgánicos (paja, restos de poda de vid y sustrato post-cultivo de hongos), comparados con tratamientos convencionales (herbicida y laboreo); ii) uso de cinco portainjertos bajo condiciones de riego y estrés hídrico; y iii) riego con agua ozonizada. Se recolectaron un total de 246 muestras de suelo, rizosfera y mosto. Se extrajo ADN total y se analizó utilizando tecnologías NGS: metabarcodificación (16S rRNA e ITS2) para el perfil taxonómico, y metagenómica de escopeta para el análisis funcional. Los resultados mostraron que la añada y la ubicación fueron los principales factores que influyeron en la microbiota bacteriana y fúngica tanto del suelo como del mosto. Las prácticas agrícolas evaluadas tuvieron un impacto menor, aunque detectable —principalmente en el suelo y la rizosfera, más que en el mosto— y sus efectos variaron según el tipo de tratamiento y el contexto ambiental. En el experimento de acolchado orgánico, se observaron cambios significativos en la diversidad beta bacteriana y la diversidad alfa fúngica en el tercer año, lo que indica efectos a largo plazo, retrasados pero notables, de estas prácticas. Sin embargo, no se detectaron cambios relevantes en la abundancia de taxones fúngicos patógenos o beneficiosos. Además, se observó una limitada coincidencia entre la microbiota del suelo y del mosto, excepto por algunos taxones fúngicos fermentativos, lo que cuestiona la hipótesis de migración microbiana del suelo al mosto. En el ensayo de portainjertos, el riego emergió como el principal factor modulador de las comunidades bacterianas de la rizosfera. No obstante, bajo condiciones de sequía, surgieron diferencias específicas de genotipo, lo que destaca el papel del portainjerto en escenarios de estrés. También se identificaron taxones microbianos clave y funciones asociadas a la sequía, que pueden ser de gran interés en investigaciones futuras. Finalmente, el riego con agua ozonizada no afectó la microbiota bacteriana y fúngica del suelo, incluidos los grupos bacterianos y fúngicos beneficiosos (PGPB) y los patógenos (GTD), lo que plantea interrogantes sobre el potencial del ozono como estrategia de riego sostenible. En conclusión, el impacto de las prácticas agrícolas sobre la microbiota del viñedo es variable y dependiente del contexto, requiriendo a menudo varios años para hacerse evidente. Los efectos observados también difieren entre grupos microbianos, con respuestas distintas entre bacterias y hongos. Esta tesis subraya la importancia de evaluar las prácticas emergentes durante varios años y bajo condiciones específicas para comprender mejor y optimizar el manejo vitícola desde una perspectiva microbiológica."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Impact assessment of different agricultural practices in grapevine soil and must microbiota"]}]}],"canonical_facts":{"dc:contributor":["Pou Mir, Alicia (Universidad de La Rioja)"],"dc:creator":["Labarga Varona, David"],"dc:date":["2026"],"dc:description":["Viticulture, an agricultural activity of great economic, social and cultural relevance, currently faces challenges that compromise its viability, such as climate change, soil degradation, biodiversity loss and the environmental impact of conventional practices. In this context, the microbiota within the viticultural ecosystem plays a critical role in crop health and wine quality. Thanks to next-generation sequencing (NGS) technologies, it is now possible to characterize the composition and functionality of these microbial communities. However, most studies on emerging practices in viticulture have not incorporated a microbiological perspective, limiting our understanding of their real impact on the agroecosystem. In this thesis, we hypothesized that agricultural practices significantly influence the structure and functionality of vineyard microbial communities. The main objective of the thesis was to assess the impact of emerging vineyard management strategies on microbial ecosystems. Specifically, the study aimed to: i) evaluate the effect of organic mulches on the fungal and bacterial communities of soil and must, ii) characterize the impact of five rootstocks under irrigation and drought conditions on the rhizosphere bacterial community and iii) determine how irrigation with ozonated water influences soil bacterial and fungal populations. Three field studies were conducted between 2019 and 2024 in vineyards within the Rioja Qualified Designation of Origin (DOCa) in three representative locations — Logroño, Aldeanueva de Ebro, and Arenzana de Abajo — selected for their edaphoclimatic conditions and vineyard management practices. The three management practices included: i) application of organic mulches (straw, grapevine pruning debris and spent mushroom post), compared with conventional treatments (herbicide and tillage); ii) use of five rootstocks under irrigation and water stress conditions; and iii) irrigation with ozonized water. A total of 246 samples from soil, rhizosphere and must were collected. Total DNA was extracted and analysed using NGS technologies: metabarcoding (16S rRNA and ITS2) for taxonomic profiling, and shotgun metagenomics for functional analysis. The results showed that vintage and location were the primary factors influencing both bacterial and fungal microbiota in soil and must. The evaluated agricultural practices had a smaller, though detectable, impact —primarily in soil and rhizosphere, rather than in must— and their effects varied depending on the treatment type and environmental context. In the organic mulch experiment, significant changes in bacterial beta diversity and fungal alpha diversity were observed in the third year, indicating delayed but notable long-term effects of these practices. However, no relevant changes in the abundance of pathogenic or beneficial fungal taxa were detected. Additionally, there was limited overlap between soil and must microbiota, except for some fermentative fungal taxa, which challenge the hypothesis of microbial migration from soil to must. In the rootstock trial, irrigation emerged as the main modulating factor on rhizosphere bacterial communities. However, under drought conditions, genotype-specific differences emerged, highlighting the role of rootstock in stress scenarios. Key microbial taxa and functions associated with drought were also identified, which may be of great interest in future research. Finally, irrigation with ozonated water did not affect soil bacterial and fungal microbiota, including beneficial bacterial and fungal groups (PGPB) and pathogens (GTD), which raises questions about the potential of ozone as a sustainable irrigation strategy. In conclusion, the impact of agricultural practices on vineyard microbiota is variable and context-dependent, often requiring multiple years to become evident. The effects observed also differ between microbial groups, with bacteria and fungi responding differently. This thesis underscores the importance of evaluating emerging practices over multiple years and under specific conditions to better understand and optimize viticultural management through a microbiological lens.","La viticultura, una actividad agrícola de gran relevancia económica, social y cultural, enfrenta actualmente desafíos que comprometen su viabilidad, como el cambio climático, la degradación del suelo, la pérdida de biodiversidad y el impacto ambiental de las prácticas convencionales. En este contexto, la microbiota dentro del ecosistema vitícola desempeña un papel crítico en la salud del cultivo y la calidad del vino. Gracias a las tecnologías de secuenciación de nueva generación (NGS), ahora es posible caracterizar la composición y funcionalidad de estas comunidades microbianas. Sin embargo, la mayoría de los estudios sobre prácticas emergentes en viticultura no han incorporado una perspectiva microbiológica, lo que limita nuestra comprensión de su impacto real en el agroecosistema. En esta tesis, se planteó la hipótesis de que las prácticas agrícolas influyen significativamente en la estructura y funcionalidad de las comunidades microbianas del viñedo. El objetivo principal de la tesis fue evaluar el impacto de estrategias emergentes de manejo del viñedo sobre los ecosistemas microbianos. Específicamente, el estudio se propuso: i) evaluar el efecto de acolchados orgánicos sobre las comunidades fúngicas y bacterianas del suelo y el mosto, ii) caracterizar el impacto de cinco portainjertos bajo condiciones de riego y sequía sobre la comunidad bacteriana de la rizosfera, y iii) determinar cómo influye el riego con agua ozonizada en las poblaciones bacterianas y fúngicas del suelo. Se realizaron tres estudios de campo entre 2019 y 2024 en viñedos dentro de la Denominación de Origen Calificada (DOCa) Rioja, en tres localidades representativas —Logroño, Aldeanueva de Ebro y Arenzana de Abajo— seleccionadas por sus condiciones edafoclimáticas y prácticas de manejo del viñedo. Las tres prácticas de manejo incluyeron: i) aplicación de acolchados orgánicos (paja, restos de poda de vid y sustrato post-cultivo de hongos), comparados con tratamientos convencionales (herbicida y laboreo); ii) uso de cinco portainjertos bajo condiciones de riego y estrés hídrico; y iii) riego con agua ozonizada. Se recolectaron un total de 246 muestras de suelo, rizosfera y mosto. Se extrajo ADN total y se analizó utilizando tecnologías NGS: metabarcodificación (16S rRNA e ITS2) para el perfil taxonómico, y metagenómica de escopeta para el análisis funcional. Los resultados mostraron que la añada y la ubicación fueron los principales factores que influyeron en la microbiota bacteriana y fúngica tanto del suelo como del mosto. Las prácticas agrícolas evaluadas tuvieron un impacto menor, aunque detectable —principalmente en el suelo y la rizosfera, más que en el mosto— y sus efectos variaron según el tipo de tratamiento y el contexto ambiental. En el experimento de acolchado orgánico, se observaron cambios significativos en la diversidad beta bacteriana y la diversidad alfa fúngica en el tercer año, lo que indica efectos a largo plazo, retrasados pero notables, de estas prácticas. Sin embargo, no se detectaron cambios relevantes en la abundancia de taxones fúngicos patógenos o beneficiosos. Además, se observó una limitada coincidencia entre la microbiota del suelo y del mosto, excepto por algunos taxones fúngicos fermentativos, lo que cuestiona la hipótesis de migración microbiana del suelo al mosto. En el ensayo de portainjertos, el riego emergió como el principal factor modulador de las comunidades bacterianas de la rizosfera. No obstante, bajo condiciones de sequía, surgieron diferencias específicas de genotipo, lo que destaca el papel del portainjerto en escenarios de estrés. También se identificaron taxones microbianos clave y funciones asociadas a la sequía, que pueden ser de gran interés en investigaciones futuras. Finalmente, el riego con agua ozonizada no afectó la microbiota bacteriana y fúngica del suelo, incluidos los grupos bacterianos y fúngicos beneficiosos (PGPB) y los patógenos (GTD), lo que plantea interrogantes sobre el potencial del ozono como estrategia de riego sostenible. En conclusión, el impacto de las prácticas agrícolas sobre la microbiota del viñedo es variable y dependiente del contexto, requiriendo a menudo varios años para hacerse evidente. Los efectos observados también difieren entre grupos microbianos, con respuestas distintas entre bacterias y hongos. Esta tesis subraya la importancia de evaluar las prácticas emergentes durante varios años y bajo condiciones específicas para comprender mejor y optimizar el manejo vitícola desde una perspectiva microbiológica."],"dc:format":["application/pdf"],"dc:identifier":["https://dialnet.unirioja.es/servlet/oaites?codigo=398291"],"dc:language":["eng"],"dc:publisher":["Universidad de La Rioja (España)"],"dc:rights":["LICENCIA DE USO: Los documentos a texto completo incluidos en Dialnet son de acceso libre y propiedad de sus autores y/o editores. Por tanto, cualquier acto de reproducción, distribución, comunicación pública y/o transformación total o parcial requiere el consentimiento expreso y escrito de aquéllos. Cualquier enlace al texto completo de estos documentos deberá hacerse a través de la URL oficial de éstos en Dialnet. Más información: https://dialnet.unirioja.es/info/derechosOAI | INTELLECTUAL PROPERTY RIGHTS STATEMENT: Full text documents hosted by Dialnet are protected by copyright and/or related rights. This digital object is accessible without charge, but its use is subject to the licensing conditions set by its authors or editors. Unless expressly stated otherwise in the licensing conditions, you are free to linking, browsing, printing and making a copy for your own personal purposes. All other acts of reproduction and communication to the public are subject to the licensing conditions expressed by editors and authors and require consent from them. Any link to this document should be made using its official URL in Dialnet. More info: https://dialnet.unirioja.es/info/derechosOAI"],"dc:title":["Impact assessment of different agricultural practices in grapevine soil and must microbiota"],"dc:type":["text (thesis)"]},"updated_at":"2026-07-24T06:27:11Z"}