During angiogenesis, the development of new blood vessels from existing vessels, there must be tight regulation between a leading tip cell and trailing stalk cells during vascular patterning. Lateral inhibition produced by Notch signaling is central to the specification and maintenance of tip and stalk cells in growing angiogenic sprouts. The Notch pathway is activated by the ligand Delta at adherens junctions between tip and stalk cells. Despite the centrality of Delta/Notch signaling to angiogenesis, relatively little is known about the mechanism of this signaling event. It has been proposed that the force generated by the Notch bound Delta ligand exposes a cleavage site necessary for Notch activation. Here, we show for the first time in endothelial cells that the proteins Epsin homology domain protein 2 (EHD2) and EHD2 binding partner 1 (EHBP1) regulate Delta like ligand 4 (Dll4) endocytosis by tethering its caveolar endocytic pit to the actin cytoskeleton.
","abstract_html":"<p>During angiogenesis, the development of new blood vessels from existing vessels, there must be tight regulation between a leading tip cell and trailing stalk cells during vascular patterning. Lateral inhibition produced by Notch signaling is central to the specification and maintenance of tip and stalk cells in growing angiogenic sprouts. The Notch pathway is activated by the ligand Delta at adherens junctions between tip and stalk cells. Despite the centrality of Delta/Notch signaling to angiogenesis, relatively little is known about the mechanism of this signaling event. It has been proposed that the force generated by the Notch bound Delta ligand exposes a cleavage site necessary for Notch activation. Here, we show for the first time in endothelial cells that the proteins Epsin homology domain protein 2 (EHD2) and EHD2 binding partner 1 (EHBP1) regulate Delta like ligand 4 (Dll4) endocytosis by tethering its caveolar endocytic pit to the actin cytoskeleton.</p>","abstract_has_math":false,"creators":["Webb, Amelia Margaret"],"institution":null,"degree_name":"M. S.","degree_level":"Masters Thesis","degree_discipline":null,"degree_department":null,"school":null,"contributors":["Erich Kushner","Martin Margittai","Schuyler Van Engelenburg","Cedric Asensio"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2020,"date_issued":"2020-01-01T08:00:00Z","date_published":"2020-01-01T08:00:00Z","updated_at":"2026-07-24T02:02:45Z","subjects":["Angiogenesis","Cell and Developmental Biology","Cell Biology"],"languages":["en"],"rights":["Copyright is held by the author. User is responsible for all copyright compliance.
"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://digitalcommons.du.edu/etd/1860","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Erich Kushner","Martin Margittai","Schuyler Van Engelenburg","Cedric Asensio"]},{"key":"dc:creator","label":"Author","values":["Webb, Amelia Margaret"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2021-04-02T07:00:00Z"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Masters Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M. S."]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Angiogenesis","Cell and Developmental Biology","Cell Biology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright is held by the author. User is responsible for all copyright compliance.
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"]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["EHBP1 and EHD2 Regulate Dll4 Caveolin-Mediated Endocytosis During Blood Vessel Development"]}]}],"canonical_facts":{"dc:contributor":["Erich Kushner","Martin Margittai","Schuyler Van Engelenburg","Cedric Asensio"],"dc:creator":["Webb, Amelia Margaret"],"dc:date.available":["2021-04-02T07:00:00Z"],"dc:description.abstract":["During angiogenesis, the development of new blood vessels from existing vessels, there must be tight regulation between a leading tip cell and trailing stalk cells during vascular patterning. Lateral inhibition produced by Notch signaling is central to the specification and maintenance of tip and stalk cells in growing angiogenic sprouts. The Notch pathway is activated by the ligand Delta at adherens junctions between tip and stalk cells. Despite the centrality of Delta/Notch signaling to angiogenesis, relatively little is known about the mechanism of this signaling event. It has been proposed that the force generated by the Notch bound Delta ligand exposes a cleavage site necessary for Notch activation. Here, we show for the first time in endothelial cells that the proteins Epsin homology domain protein 2 (EHD2) and EHD2 binding partner 1 (EHBP1) regulate Delta like ligand 4 (Dll4) endocytosis by tethering its caveolar endocytic pit to the actin cytoskeleton.
"],"dc:format":["application/pdf"],"dc:identifier":["https://digitalcommons.du.edu/etd/1860"],"dc:language":["en"],"dc:rights":["Copyright is held by the author. User is responsible for all copyright compliance.
"],"dc:subject":["Angiogenesis","Cell and Developmental Biology","Cell Biology"],"dc:title":["EHBP1 and EHD2 Regulate Dll4 Caveolin-Mediated Endocytosis During Blood Vessel Development"],"thesis:degree_level":["Masters Thesis"],"thesis:degree_name":["M. S."]},"updated_at":"2026-07-24T02:02:45Z"}