Ethylene (ethene) is the essential ripening hormone for climacteric plants, and consequently its detection and quantification is of considerable interest to the horticulture industry. Its detection however is a non-trivial pursuit, traditionally requiring expensive and inaccessible instrumentation that fail to provide in vivo information about ethylene. A ruthenium based fluorescent probe for the selective detection of ethylene has recently been developed, and it was envisioned that this probe could be made biocompatible by encasing it within a protein scaffold making it an artificial metalloenzyme. To this end biotinylated N-heterocyclic carbenes (NHCs) were synthesized, which can be appended onto the ruthenium probe. Efforts towards the expression and purification of the protein scaffold are also here reported.
Mycofactocin (MFT) is a ribosomally synthesized and post-translationally modified peptide (RiPP) hypothesized to function as a redox cofactor in bacteria, particularly the Mycobacteria genus, which includes M. tuberculosis. In order to further elucidate the function of MFT and its associated proteins, a synthetic route was desired to the small molecule. A viable synthetic route to MFT, pre-MFT (PMFT) and other structural analogues is here reported.
","abstract_html":"<p>Ethylene (ethene) is the essential ripening hormone for climacteric plants, and consequently its detection and quantification is of considerable interest to the horticulture industry. Its detection however is a non-trivial pursuit, traditionally requiring expensive and inaccessible instrumentation that fail to provide in vivo information about ethylene. A ruthenium based fluorescent probe for the selective detection of ethylene has recently been developed, and it was envisioned that this probe could be made biocompatible by encasing it within a protein scaffold making it an artificial metalloenzyme. To this end biotinylated N-heterocyclic carbenes (NHCs) were synthesized, which can be appended onto the ruthenium probe. Efforts towards the expression and purification of the protein scaffold are also here reported.</p> <p>Mycofactocin (MFT) is a ribosomally synthesized and post-translationally modified peptide (RiPP) hypothesized to function as a redox cofactor in bacteria, particularly the Mycobacteria genus, which includes M. tuberculosis. In order to further elucidate the function of MFT and its associated proteins, a synthetic route was desired to the small molecule. A viable synthetic route to MFT, pre-MFT (PMFT) and other structural analogues is here reported.</p>","abstract_has_math":false,"creators":["Ellinwood, Mitchell Lee"],"institution":null,"degree_name":"M. S.","degree_level":"Masters Thesis","degree_discipline":null,"degree_department":null,"school":null,"contributors":["Brian W. Michel","John Latham","Andrei Kutateladze","Scott Barbee"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2020,"date_issued":"2020-01-01T08:00:00Z","date_published":"2020-01-01T08:00:00Z","updated_at":"2026-07-24T02:02:45Z","subjects":["Ethylene","Detection","Organic chemistry","Chemistry"],"languages":["en"],"rights":["Copyright is held by the author. User is responsible for all copyright compliance.
"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://digitalcommons.du.edu/etd/1744","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Brian W. Michel","John Latham","Andrei Kutateladze","Scott Barbee"]},{"key":"dc:creator","label":"Author","values":["Ellinwood, Mitchell Lee"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2021-02-12T08:00:00Z"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Masters Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M. S."]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Ethylene","Detection","Organic chemistry","Chemistry"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright is held by the author. User is responsible for all copyright compliance.
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Mycofactocin (MFT) is a ribosomally synthesized and post-translationally modified peptide (RiPP) hypothesized to function as a redox cofactor in bacteria, particularly the Mycobacteria genus, which includes M. tuberculosis. In order to further elucidate the function of MFT and its associated proteins, a synthetic route was desired to the small molecule. A viable synthetic route to MFT, pre-MFT (PMFT) and other structural analogues is here reported.
"]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Development Towards an Ethylene Sensing Artificial Metalloenzyme"]}]}],"canonical_facts":{"dc:contributor":["Brian W. Michel","John Latham","Andrei Kutateladze","Scott Barbee"],"dc:creator":["Ellinwood, Mitchell Lee"],"dc:date.available":["2021-02-12T08:00:00Z"],"dc:description.abstract":["Ethylene (ethene) is the essential ripening hormone for climacteric plants, and consequently its detection and quantification is of considerable interest to the horticulture industry. Its detection however is a non-trivial pursuit, traditionally requiring expensive and inaccessible instrumentation that fail to provide in vivo information about ethylene. A ruthenium based fluorescent probe for the selective detection of ethylene has recently been developed, and it was envisioned that this probe could be made biocompatible by encasing it within a protein scaffold making it an artificial metalloenzyme. To this end biotinylated N-heterocyclic carbenes (NHCs) were synthesized, which can be appended onto the ruthenium probe. Efforts towards the expression and purification of the protein scaffold are also here reported.
Mycofactocin (MFT) is a ribosomally synthesized and post-translationally modified peptide (RiPP) hypothesized to function as a redox cofactor in bacteria, particularly the Mycobacteria genus, which includes M. tuberculosis. In order to further elucidate the function of MFT and its associated proteins, a synthetic route was desired to the small molecule. A viable synthetic route to MFT, pre-MFT (PMFT) and other structural analogues is here reported.
"],"dc:format":["application/pdf"],"dc:identifier":["https://digitalcommons.du.edu/etd/1744"],"dc:language":["en"],"dc:rights":["Copyright is held by the author. User is responsible for all copyright compliance.
"],"dc:subject":["Ethylene","Detection","Organic chemistry","Chemistry"],"dc:title":["Development Towards an Ethylene Sensing Artificial Metalloenzyme"],"thesis:degree_level":["Masters Thesis"],"thesis:degree_name":["M. S."]},"updated_at":"2026-07-24T02:02:45Z"}