Motion of particles under influencing forces may be observed under light microscopy techniques. Variations in mobility of particles may give relevant biophysical information. Automated high resolution single particle tracking techniques were used to characterize interphase chromatin mobility in the cell nucleus. Interphase chromatin undergo replication prior to cell division with the assistance of replication proteins (machinery) which modify chromatin mobility. Using dual color imaging of flourescently tagged chromatin and proliferating cell nuclear antigen (PCNA) were followed through interphase. Chromatin motion was modelled as a two dimensional random walk. Reduction in chromatin mobility was observed during S phase was dependent on proximity to the replication machinery. Mobility during G1 and G2 phase is independent of proximity to GFP-PCNA maxima and is higher than during S phase. These results suggest that replication selectively inhibits chromatin mobility. Local proximity to replication machinery however, cannot account for the entire mobility difference. By modelling the mean square displacement as a power law, sub-diffusive behavior was observed in S phase chromatin whereas non S phase exhibited normal diffusional characteristics.
","abstract_html":"<p>Motion of particles under influencing forces may be observed under light microscopy techniques. Variations in mobility of particles may give relevant biophysical information. Automated high resolution single particle tracking techniques were used to characterize interphase chromatin mobility in the cell nucleus. Interphase chromatin undergo replication prior to cell division with the assistance of replication proteins (machinery) which modify chromatin mobility. Using dual color imaging of flourescently tagged chromatin and proliferating cell nuclear antigen (PCNA) were followed through interphase. Chromatin motion was modelled as a two dimensional random walk. Reduction in chromatin mobility was observed during S phase was dependent on proximity to the replication machinery. Mobility during G1 and G2 phase is independent of proximity to GFP-PCNA maxima and is higher than during S phase. These results suggest that replication selectively inhibits chromatin mobility. Local proximity to replication machinery however, cannot account for the entire mobility difference. By modelling the mean square displacement as a power law, sub-diffusive behavior was observed in S phase chromatin whereas non S phase exhibited normal diffusional characteristics.</p>","abstract_has_math":false,"creators":["Westacott, Matthew Joel"],"institution":null,"degree_name":"M.S.","degree_level":"Masters Thesis","degree_discipline":null,"degree_department":null,"school":null,"contributors":["Dinah Loerke, Ph.D.","Mark Siemens","Kingshuk Ghosh"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2012,"date_issued":"2012-01-01T08:00:00Z","date_published":"2012-01-01T08:00:00Z","updated_at":"2026-07-24T02:02:19Z","subjects":["Motion of particles","Chromatin motion","Proliferating cell nuclear antigen","Atomic, Molecular and Optical Physics","Physical Sciences and Mathematics","Physics"],"languages":["en"],"rights":["Copyright is held by the author. User is responsible for all copyright compliance.
"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://digitalcommons.du.edu/etd/700","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Dinah Loerke, Ph.D.","Mark Siemens","Kingshuk Ghosh"]},{"key":"dc:creator","label":"Author","values":["Westacott, Matthew Joel"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2001-01-01T08:00:00Z"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Masters Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Motion of particles","Chromatin motion","Proliferating cell nuclear antigen","Atomic, Molecular and Optical Physics","Physical Sciences and Mathematics","Physics"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright is held by the author. User is responsible for all copyright compliance.
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"]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Analysis of Interphase Chromatin Motion in HeLa Cells"]}]}],"canonical_facts":{"dc:contributor":["Dinah Loerke, Ph.D.","Mark Siemens","Kingshuk Ghosh"],"dc:creator":["Westacott, Matthew Joel"],"dc:date.available":["2001-01-01T08:00:00Z"],"dc:description.abstract":["Motion of particles under influencing forces may be observed under light microscopy techniques. Variations in mobility of particles may give relevant biophysical information. Automated high resolution single particle tracking techniques were used to characterize interphase chromatin mobility in the cell nucleus. Interphase chromatin undergo replication prior to cell division with the assistance of replication proteins (machinery) which modify chromatin mobility. Using dual color imaging of flourescently tagged chromatin and proliferating cell nuclear antigen (PCNA) were followed through interphase. Chromatin motion was modelled as a two dimensional random walk. Reduction in chromatin mobility was observed during S phase was dependent on proximity to the replication machinery. Mobility during G1 and G2 phase is independent of proximity to GFP-PCNA maxima and is higher than during S phase. These results suggest that replication selectively inhibits chromatin mobility. Local proximity to replication machinery however, cannot account for the entire mobility difference. By modelling the mean square displacement as a power law, sub-diffusive behavior was observed in S phase chromatin whereas non S phase exhibited normal diffusional characteristics.
"],"dc:format":["application/pdf"],"dc:identifier":["https://digitalcommons.du.edu/etd/700"],"dc:language":["en"],"dc:rights":["Copyright is held by the author. User is responsible for all copyright compliance.
"],"dc:subject":["Motion of particles","Chromatin motion","Proliferating cell nuclear antigen","Atomic, Molecular and Optical Physics","Physical Sciences and Mathematics","Physics"],"dc:title":["Analysis of Interphase Chromatin Motion in HeLa Cells"],"thesis:degree_level":["Masters Thesis"],"thesis:degree_name":["M.S."]},"updated_at":"2026-07-24T02:02:19Z"}