Transposable elements (TE) have been found in all genomes and have clearly had a major impact on genomic evolution. The described research takes advantage of an abundant transposable element present in the genomes of Anseriformes, called Chicken Repeat 1 (CR1). Previous studies in Anseriformes suggest that CR1 is presently active in recent evolutionary time (St. John, 2004). A fully functional CR1 element itself is approximately 4.5kb long (Kajikawa, 1997), where almost all inserts are truncated at the 5' end. Because of this, it has been a challenge to isolate a full length, active element. In this study, two CR1 sequences were obtained after screening a genomic library of Cape Barren Goose using probes complimentary to the flanking regions of the element. The findings unveiled sequences with a complete ORF1, ORF2, 3' untranslated region and a portion of the 5' untranslated region. This study gets one step closer a further understanding the transposition mechanism that are adopted by this class of TE's, a non-long terminal repeat (non-LTR) retransposons. Eventually, the capture of an active element could make it especially valuable for future research by investigating their ability to transpose in living cells.
","abstract_html":"<p>Transposable elements (TE) have been found in all genomes and have clearly had a major impact on genomic evolution. The described research takes advantage of an abundant transposable element present in the genomes of Anseriformes, called Chicken Repeat 1 (CR1). Previous studies in Anseriformes suggest that CR1 is presently active in recent evolutionary time (St. John, 2004). A fully functional CR1 element itself is approximately 4.5kb long (Kajikawa, 1997), where almost all inserts are truncated at the 5' end. Because of this, it has been a challenge to isolate a full length, active element. In this study, two CR1 sequences were obtained after screening a genomic library of Cape Barren Goose using probes complimentary to the flanking regions of the element. The findings unveiled sequences with a complete ORF1, ORF2, 3' untranslated region and a portion of the 5' untranslated region. This study gets one step closer a further understanding the transposition mechanism that are adopted by this class of TE's, a non-long terminal repeat (non-LTR) retransposons. Eventually, the capture of an active element could make it especially valuable for future research by investigating their ability to transpose in living cells.</p>","abstract_has_math":false,"creators":["Weason, Cassandra Michelle"],"institution":null,"degree_name":"M.S.","degree_level":"Masters Thesis","degree_discipline":null,"degree_department":null,"school":null,"contributors":["Thomas W. Quinn, Ph.D.","Robert Dores","Nancy Sasaki","Alex Huffman"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2013,"date_issued":"2013-01-01T08:00:00Z","date_published":"2013-01-01T08:00:00Z","updated_at":"2026-07-24T02:03:03Z","subjects":["Transposable elements","TE","Genomic evolution","Transposition mechanism","Genetics","Genetics and Genomics","Life Sciences"],"languages":["en"],"rights":["Copyright is held by the author. User is responsible for all copyright compliance.
"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://digitalcommons.du.edu/etd/691","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Thomas W. Quinn, Ph.D.","Robert Dores","Nancy Sasaki","Alex Huffman"]},{"key":"dc:creator","label":"Author","values":["Weason, Cassandra Michelle"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2001-01-01T08:00:00Z"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Masters Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Transposable elements","TE","Genomic evolution","Transposition mechanism","Genetics","Genetics and Genomics","Life Sciences"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright is held by the author. User is responsible for all copyright compliance.
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"],"dc:format":["application/pdf"],"dc:identifier":["https://digitalcommons.du.edu/etd/691"],"dc:language":["en"],"dc:rights":["Copyright is held by the author. User is responsible for all copyright compliance.
"],"dc:subject":["Transposable elements","TE","Genomic evolution","Transposition mechanism","Genetics","Genetics and Genomics","Life Sciences"],"dc:title":["Isolation and Characterization of a Full Length Retrotransposon: CR1"],"thesis:degree_level":["Masters Thesis"],"thesis:degree_name":["M.S."]},"updated_at":"2026-07-24T02:03:03Z"}