P2X2 receptors and P2Y4 receptors are ATP-activated cell surface receptors that gate movement of K+, Na+, and Ca2+. We used immunocytochemistry for P2X2 and P2Y4 receptors as well as taste cell type markers to learn if P2X2 immunoreactivity (IR) is present in nerve processes in close apposition to Type II and/or Type III taste cells. We also tested to see if P2Y4 IR is present in Type II and/or Type III taste cells. Our results indicate that P2X2 is present only in intragemmal nerve processes. P2X2-LIR nerve processes form close contacts with Type II and Type III taste cells. P2Y4 IR is present in Type II cells that also display IP3R3 IR, and in Type III cells with Syntaxin-1 IR and 5-HT IR. These data suggest that ATP stimulates P2X2 receptors on nerve processes and P2Y4 receptors present on both Type II cells and Type III cells.
","abstract_html":"<p>P2X2 receptors and P2Y4 receptors are ATP-activated cell surface receptors that gate movement of K+, Na+, and Ca2+. We used immunocytochemistry for P2X2 and P2Y4 receptors as well as taste cell type markers to learn if P2X2 immunoreactivity (IR) is present in nerve processes in close apposition to Type II and/or Type III taste cells. We also tested to see if P2Y4 IR is present in Type II and/or Type III taste cells. Our results indicate that P2X2 is present only in intragemmal nerve processes. P2X2-LIR nerve processes form close contacts with Type II and Type III taste cells. P2Y4 IR is present in Type II cells that also display IP3R3 IR, and in Type III cells with Syntaxin-1 IR and 5-HT IR. These data suggest that ATP stimulates P2X2 receptors on nerve processes and P2Y4 receptors present on both Type II cells and Type III cells.</p>","abstract_has_math":false,"creators":["Montoya, Alana Marselle"],"institution":null,"degree_name":"M.S.","degree_level":"Masters Thesis","degree_discipline":null,"degree_department":null,"school":null,"contributors":["John C. Kinnamon, Ph.D.","Joseph Angleson","Christina Coughlan"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2008,"date_issued":"2008-01-01T08:00:00Z","date_published":"2008-01-01T08:00:00Z","updated_at":"2026-07-24T02:03:12Z","subjects":["ATP","Connexin","Pannexin","Purinergic receptors","Taste","Biology","Cell and Developmental Biology"],"languages":["en"],"rights":["Copyright is held by the author. User is responsible for all copyright compliance.
"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://digitalcommons.du.edu/etd/442","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["John C. Kinnamon, Ph.D.","Joseph Angleson","Christina Coughlan"]},{"key":"dc:creator","label":"Author","values":["Montoya, Alana Marselle"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2001-01-01T08:00:00Z"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Masters Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["ATP","Connexin","Pannexin","Purinergic receptors","Taste","Biology","Cell and Developmental Biology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright is held by the author. User is responsible for all copyright compliance.
"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://digitalcommons.du.edu/etd/442"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["P2X2 receptors and P2Y4 receptors are ATP-activated cell surface receptors that gate movement of K+, Na+, and Ca2+. We used immunocytochemistry for P2X2 and P2Y4 receptors as well as taste cell type markers to learn if P2X2 immunoreactivity (IR) is present in nerve processes in close apposition to Type II and/or Type III taste cells. We also tested to see if P2Y4 IR is present in Type II and/or Type III taste cells. Our results indicate that P2X2 is present only in intragemmal nerve processes. P2X2-LIR nerve processes form close contacts with Type II and Type III taste cells. P2Y4 IR is present in Type II cells that also display IP3R3 IR, and in Type III cells with Syntaxin-1 IR and 5-HT IR. These data suggest that ATP stimulates P2X2 receptors on nerve processes and P2Y4 receptors present on both Type II cells and Type III cells.
"]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Purinergic Receptors in the Circumvallate Papillae of Rats"]}]}],"canonical_facts":{"dc:contributor":["John C. Kinnamon, Ph.D.","Joseph Angleson","Christina Coughlan"],"dc:creator":["Montoya, Alana Marselle"],"dc:date.available":["2001-01-01T08:00:00Z"],"dc:description.abstract":["P2X2 receptors and P2Y4 receptors are ATP-activated cell surface receptors that gate movement of K+, Na+, and Ca2+. We used immunocytochemistry for P2X2 and P2Y4 receptors as well as taste cell type markers to learn if P2X2 immunoreactivity (IR) is present in nerve processes in close apposition to Type II and/or Type III taste cells. We also tested to see if P2Y4 IR is present in Type II and/or Type III taste cells. Our results indicate that P2X2 is present only in intragemmal nerve processes. P2X2-LIR nerve processes form close contacts with Type II and Type III taste cells. P2Y4 IR is present in Type II cells that also display IP3R3 IR, and in Type III cells with Syntaxin-1 IR and 5-HT IR. These data suggest that ATP stimulates P2X2 receptors on nerve processes and P2Y4 receptors present on both Type II cells and Type III cells.
"],"dc:format":["application/pdf"],"dc:identifier":["https://digitalcommons.du.edu/etd/442"],"dc:language":["en"],"dc:rights":["Copyright is held by the author. User is responsible for all copyright compliance.
"],"dc:subject":["ATP","Connexin","Pannexin","Purinergic receptors","Taste","Biology","Cell and Developmental Biology"],"dc:title":["Purinergic Receptors in the Circumvallate Papillae of Rats"],"thesis:degree_level":["Masters Thesis"],"thesis:degree_name":["M.S."]},"updated_at":"2026-07-24T02:03:12Z"}