Using CHO cells we sought to explore and characterize the functional relationship of rainbow trout MC5 receptor (rtMC5R) with zebrafish MRAP1 (zfMRAP1) and rainbow trout MC2 receptor (rtMC2R), as well as how the effect of such relationships may play a significant role in Hypothalamic-Pituitary-Adrenal/Interrenal axis activation. This research demonstrated that rtMC5R can be successfully expressed and functionally activated in CHO cells and, in a manner similar to mammals, α-MSH is the preferred ligand for rtMC5R. The presence of MRAP1 does not seem to inhibit the expression or function of rtMC5R, rather it appears that it may increase expression and total activation levels. In contrast, the presence of MRAP1 in conjunction with MC2R reduces expression and total activation levels of rtMC5R. Findings indicate that rtMC5R may function in three different ways in a cell, depending upon the presence of other local proteins. The interaction of rtMC5R with MRAP1 and MC2R, and the implications of such interactions will be described in this thesis.
","abstract_html":"<p>Using CHO cells we sought to explore and characterize the functional relationship of rainbow trout MC5 receptor (rtMC5R) with zebrafish MRAP1 (zfMRAP1) and rainbow trout MC2 receptor (rtMC2R), as well as how the effect of such relationships may play a significant role in Hypothalamic-Pituitary-Adrenal/Interrenal axis activation. This research demonstrated that rtMC5R can be successfully expressed and functionally activated in CHO cells and, in a manner similar to mammals, α-MSH is the preferred ligand for rtMC5R. The presence of MRAP1 does not seem to inhibit the expression or function of rtMC5R, rather it appears that it may increase expression and total activation levels. In contrast, the presence of MRAP1 in conjunction with MC2R reduces expression and total activation levels of rtMC5R. Findings indicate that rtMC5R may function in three different ways in a cell, depending upon the presence of other local proteins. The interaction of rtMC5R with MRAP1 and MC2R, and the implications of such interactions will be described in this thesis.</p>","abstract_has_math":false,"creators":["Hill, Domicinda M."],"institution":null,"degree_name":"M.S.","degree_level":"Masters Thesis","degree_discipline":null,"degree_department":null,"school":null,"contributors":["Robert M. Dores, Ph.D.","Daniel Linseman","Nancy Lorenzon","Scott Pegan"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2012,"date_issued":"2012-06-01T07:00:00Z","date_published":"2012-06-01T07:00:00Z","updated_at":"2026-07-24T02:02:45Z","subjects":["MC2R","MC5R","MRAP1","Rainbow Trout","Biology","Endocrinology"],"languages":["en"],"rights":["Copyright is held by the author. User is responsible for all copyright compliance.
"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://digitalcommons.du.edu/etd/288","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Robert M. Dores, Ph.D.","Daniel Linseman","Nancy Lorenzon","Scott Pegan"]},{"key":"dc:creator","label":"Author","values":["Hill, Domicinda M."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2001-01-01T08:00:00Z"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Masters Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["MC2R","MC5R","MRAP1","Rainbow Trout","Biology","Endocrinology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright is held by the author. User is responsible for all copyright compliance.
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"]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Analyzing the Interaction Between Melanocortin 5 Receptor, Melanocortin Receptor Accessory Protein 1, and Melanocortin 2 Receptor in Rainbow Trout"]}]}],"canonical_facts":{"dc:contributor":["Robert M. Dores, Ph.D.","Daniel Linseman","Nancy Lorenzon","Scott Pegan"],"dc:creator":["Hill, Domicinda M."],"dc:date.available":["2001-01-01T08:00:00Z"],"dc:description.abstract":["Using CHO cells we sought to explore and characterize the functional relationship of rainbow trout MC5 receptor (rtMC5R) with zebrafish MRAP1 (zfMRAP1) and rainbow trout MC2 receptor (rtMC2R), as well as how the effect of such relationships may play a significant role in Hypothalamic-Pituitary-Adrenal/Interrenal axis activation. This research demonstrated that rtMC5R can be successfully expressed and functionally activated in CHO cells and, in a manner similar to mammals, α-MSH is the preferred ligand for rtMC5R. The presence of MRAP1 does not seem to inhibit the expression or function of rtMC5R, rather it appears that it may increase expression and total activation levels. In contrast, the presence of MRAP1 in conjunction with MC2R reduces expression and total activation levels of rtMC5R. Findings indicate that rtMC5R may function in three different ways in a cell, depending upon the presence of other local proteins. The interaction of rtMC5R with MRAP1 and MC2R, and the implications of such interactions will be described in this thesis.
"],"dc:format":["application/pdf"],"dc:identifier":["https://digitalcommons.du.edu/etd/288"],"dc:language":["en"],"dc:rights":["Copyright is held by the author. User is responsible for all copyright compliance.
"],"dc:subject":["MC2R","MC5R","MRAP1","Rainbow Trout","Biology","Endocrinology"],"dc:title":["Analyzing the Interaction Between Melanocortin 5 Receptor, Melanocortin Receptor Accessory Protein 1, and Melanocortin 2 Receptor in Rainbow Trout"],"thesis:degree_level":["Masters Thesis"],"thesis:degree_name":["M.S."]},"updated_at":"2026-07-24T02:02:45Z"}