Investigation of biofilm development by antibiotic resistant urinary tract infection pathogens and evaluation of antimicrobial activity of Manuka honey on Escherichia coli CTX-M-15 biofilms
type: CreativeWork
description: Background Antibiotic resistance is one of the major healthcare issues worldwide. Over time, bacteria are becoming more resistant, making antibiotics less effective, and this in turn will lead to an increase in the morbidity rate, the mortality rate, and the healthcare system expenses as well. The rise of antibiotic resistance is reaching critical levels as new mechanisms of resistance are emerging and spreading globally, limiting the available effective treatment options. In addition, biofilms are another significant health care issue: they act as a protective cover for infectious microbes. They are also hard to remove: they require a much higher dose of antibiotics than planktonic organisms. When treating biofilm-associated infections, it is important to understand the biology of biofilm development and biofilm-related gene expression. To understand the role of biofilm in pathogenesis, biofilm formation and gene expression in Escherichia coli CTX-M-15 were investigated in this study. The antivirulence efficacy of Manuka honey as an alternative treatment was analysed for this strain. Method Biofilm formation was measured using the Tissue Culture plate method at different times: after 6, 12, 24 and 48 hours of incubation. Assuming that times correlated with the stages of biofilm development, initial attachment occurred at 6 hours, microcolonies formed at 12 hours, biofilm maturation occurred at 24 hours and biofilm dispersion occurred at 48 hours. Biofilms were quantified in both static and shaking incubators and under three different growth media: nutrient-poor (AB broth), nutrient-rich (Luria-Bertani broth) and general (nutrient broth). A quantitative Polymerase Chain Reaction was used to determine the biofilm-related gene expression at the in vitro biofilm development stages. It was also used to determine the antivirulence effect of Manuka honey on select biofilm-related gene expressions. Results The tested strains showed a variable biofilm formation ability when…
abstract: Background Antibiotic resistance is one of the major healthcare issues worldwide. Over time, bacteria are becoming more resistant, making antibiotics less effective, and this in turn will lead to an increase in the morbidity rate, the mortality rate, and the healthcare system expenses as well. The rise of antibiotic resistance is reaching critical levels as new mechanisms of resistance are emerging and spreading globally, limiting the available effective treatment options. In addition, biofilms are another significant health care issue: they act as a protective cover for infectious microbes. They are also hard to remove: they require a much higher dose of antibiotics than planktonic organisms. When treating biofilm-associated infections, it is important to understand the biology of biofilm development and biofilm-related gene expression. To understand the role of biofilm in pathogenesis, biofilm formation and gene expression in Escherichia coli CTX-M-15 were investigated in this study. The antivirulence efficacy of Manuka honey as an alternative treatment was analysed for this strain. Method Biofilm formation was measured using the Tissue Culture plate method at different times: after 6, 12, 24 and 48 hours of incubation. Assuming that times correlated with the stages of biofilm development, initial attachment occurred at 6 hours, microcolonies formed at 12 hours, biofilm maturation occurred at 24 hours and biofilm dispersion occurred at 48 hours. Biofilms were quantified in both static and shaking incubators and under three different growth media: nutrient-poor (AB broth), nutrient-rich (Luria-Bertani broth) and general (nutrient broth). A quantitative Polymerase Chain Reaction was used to determine the biofilm-related gene expression at the in vitro biofilm development stages. It was also used to determine the antivirulence effect of Manuka honey on select biofilm-related gene expressions. Results The tested strains showed a variable biofilm formation ability when…
author: Baho, Sinan
license: https://dora.dmu.ac.uk/bitstreams/765d8d24-31a1-410e-8d95-a18de82fbd52/download
date published: 2019-12
date modified: 2026-07-24T06:18:24Z
provider: De Montfort University
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: De Montfort University