An investigation into the effects of shape and structure on the antibacterial efficacy of metal nanoparticles
type: CreativeWork
description: Increasing numbers of microbial organisms are becoming resistant to antibiotics, demonstrating a need for new, effective antibacterial agents. Metal nanoparticles (NPs) such as silver (AgNPs), copper (CuNPs) and gold (AuNPs) exhibit antimicrobial properties against bacteria, including Escherichia coli and Enterococcus faecium. E. coli O157:H7, which is known to be virulent and infectious, causing bowel discomfort, diarrhoea, nausea or vomiting. Enterococci frequently causes gastrointestinal infections, urinary tract infections, hepatobiliary sepsis, endocarditis as well as hospital-acquired infections, e.g. nosocomial bacteraemia and surgical wound infection. As regards E. coli, NHS trusts in England reported 38,132 cases of bacteraemia between 1 April 2015 and 31 March 2016 (NHS, 2017). There was an overall increase in the incidence of bacteraemia caused by Enterococcus spp between 2010 and 2017, from 9.9 to 13.1 per 100,000 of the population in England respectively. The antibacterial activity of metal nanoparticles has been investigated extensively due to their high surface area-to-volume ratio and the generation of reactive oxygen species. Metal nanoparticles have potential as alternatives to current antimicrobials used in the hospital environment to combat Healthcare Associated Infections (HCAIs). Nanoparticles were synthesised using the chemical reduction method, where the size and shape were controlled via the precursor or reduction agent, reaction time, temperature and the molar ration between the precursor and reduction agent. Nanoparticles were characterised using ultra-violet/visible spectroscopy (UV-Vis), scanning electron microscopy (SEM), energy-dispersive spectroscopy (EDX), X-ray diffraction (XRD) and transmission electron microscopy (TEM). Minimum inhibitory concentration (MIC) was used to assess the antimicrobial efficacy of metal NPs. The antimicrobial activity was then determined using growth inhibition kinetics by 96 well plate reader and viable …
abstract: Increasing numbers of microbial organisms are becoming resistant to antibiotics, demonstrating a need for new, effective antibacterial agents. Metal nanoparticles (NPs) such as silver (AgNPs), copper (CuNPs) and gold (AuNPs) exhibit antimicrobial properties against bacteria, including Escherichia coli and Enterococcus faecium. E. coli O157:H7, which is known to be virulent and infectious, causing bowel discomfort, diarrhoea, nausea or vomiting. Enterococci frequently causes gastrointestinal infections, urinary tract infections, hepatobiliary sepsis, endocarditis as well as hospital-acquired infections, e.g. nosocomial bacteraemia and surgical wound infection. As regards E. coli, NHS trusts in England reported 38,132 cases of bacteraemia between 1 April 2015 and 31 March 2016 (NHS, 2017). There was an overall increase in the incidence of bacteraemia caused by Enterococcus spp between 2010 and 2017, from 9.9 to 13.1 per 100,000 of the population in England respectively. The antibacterial activity of metal nanoparticles has been investigated extensively due to their high surface area-to-volume ratio and the generation of reactive oxygen species. Metal nanoparticles have potential as alternatives to current antimicrobials used in the hospital environment to combat Healthcare Associated Infections (HCAIs). Nanoparticles were synthesised using the chemical reduction method, where the size and shape were controlled via the precursor or reduction agent, reaction time, temperature and the molar ration between the precursor and reduction agent. Nanoparticles were characterised using ultra-violet/visible spectroscopy (UV-Vis), scanning electron microscopy (SEM), energy-dispersive spectroscopy (EDX), X-ray diffraction (XRD) and transmission electron microscopy (TEM). Minimum inhibitory concentration (MIC) was used to assess the antimicrobial efficacy of metal NPs. The antimicrobial activity was then determined using growth inhibition kinetics by 96 well plate reader and viable …
author: Alshareef, Alanod
license: https://dora.dmu.ac.uk/bitstreams/93214d47-aed5-42dc-bc47-e68bb8cb8154/download
date published: 2019-07
date modified: 2026-07-24T06:18:51Z
provider: De Montfort University
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: De Montfort University