{"id":{"repo_id":"dcu","oai_identifier":"oai:doras.dcu.ie:17000"},"canonical_url":"https://search.dev.ndltd.org/etd/dcu/oai:doras.dcu.ie:17000","repository":{"repo_id":"dcu","name":"Dublin City University","base_url":"http://doras.dcu.ie/cgi/oai2"},"display":{"title":"Investigations into the sensing capabilities of an electrochemical immunosensor employing polyaniline as a mediator species","abstract":"The aim of this thesis was to expand the sensing capabilities of a conducting polymer-based sensor platform developed in our laboratory. The platform itself is composed of a polyaniline - poly(vinylsulphonate) (PANIIPVS) modified screen-printed carbon paste electrode used as a substrate for the immobilisation of antibody, and the subsequent competition assays of protein analytes. Chapter 1 is a literature review encompassing the field of electrochemical immunosensing. chapter 3 outlines work towards developing real-time imrnunosensors to oestradiol and p60. This ork involved the synthesis of horseradish peroxidase (HRP) conjugates with oestradiol, and p60 or use in a competitive immunosensor, as well as immunoassay characterisation of the proteins, nd their application to the biosensor platform. Chapter 4 outlines efforts to address sub-monolayer protein immobilisation issues to the PANIIPVS matrix by optimising experimental parameters overning the electrostatic irnmobilisation process. It also details experiments probing icroperoxidase-11 as an alternative labeling enzyme to HRP. Chapter 5 deals with the investigation of a novel surfactant-based strategy to reduce non-specific interactions between species present in the bulk solution, and the PANIIPVS matrix. This research highlights the need for developing the technology for real-time electrochemical immunosensing, and examines some of the critical issues involved.","abstract_html":"The aim of this thesis was to expand the sensing capabilities of a conducting polymer-based sensor platform developed in our laboratory. The platform itself is composed of a polyaniline - poly(vinylsulphonate) (PANIIPVS) modified screen-printed carbon paste electrode used as a substrate for the immobilisation of antibody, and the subsequent competition assays of protein analytes. Chapter 1 is a literature review encompassing the field of electrochemical immunosensing. chapter 3 outlines work towards developing real-time imrnunosensors to oestradiol and p60. This ork involved the synthesis of horseradish peroxidase (HRP) conjugates with oestradiol, and p60 or use in a competitive immunosensor, as well as immunoassay characterisation of the proteins, nd their application to the biosensor platform. Chapter 4 outlines efforts to address sub-monolayer protein immobilisation issues to the PANIIPVS matrix by optimising experimental parameters overning the electrostatic irnmobilisation process. It also details experiments probing icroperoxidase-11 as an alternative labeling enzyme to HRP. Chapter 5 deals with the investigation of a novel surfactant-based strategy to reduce non-specific interactions between species present in the bulk solution, and the PANIIPVS matrix. This research highlights the need for developing the technology for real-time electrochemical immunosensing, and examines some of the critical issues involved.","abstract_has_math":false,"creators":["Walsh, Padraic"],"institution":"Dublin City University","degree_name":"msc","degree_level":"masters","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2006,"date_issued":"2006","date_published":"2006","updated_at":"2026-07-24T02:00:50Z","subjects":["Chemical detectors"],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":null,"outbound_label":null,"outbound_source":null},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Walsh, Padraic"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2006"]},{"key":"dc:date.issued","label":"Date","values":["2006"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["Dublin City University"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://doras.dcu.ie/17000/"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["masters"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["msc"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Chemical detectors"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://doras.dcu.ie/17000/1/padraic-walsh_SC.pdf"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The aim of this thesis was to expand the sensing capabilities of a conducting polymer-based sensor platform developed in our laboratory. The platform itself is composed of a polyaniline - poly(vinylsulphonate) (PANIIPVS) modified screen-printed carbon paste electrode used as a substrate for the immobilisation of antibody, and the subsequent competition assays of protein analytes. Chapter 1 is a literature review encompassing the field of electrochemical immunosensing. chapter 3 outlines work towards developing real-time imrnunosensors to oestradiol and p60. This ork involved the synthesis of horseradish peroxidase (HRP) conjugates with oestradiol, and p60 or use in a competitive immunosensor, as well as immunoassay characterisation of the proteins, nd their application to the biosensor platform. Chapter 4 outlines efforts to address sub-monolayer protein immobilisation issues to the PANIIPVS matrix by optimising experimental parameters overning the electrostatic irnmobilisation process. It also details experiments probing icroperoxidase-11 as an alternative labeling enzyme to HRP. Chapter 5 deals with the investigation of a novel surfactant-based strategy to reduce non-specific interactions between species present in the bulk solution, and the PANIIPVS matrix. This research highlights the need for developing the technology for real-time electrochemical immunosensing, and examines some of the critical issues involved."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Investigations into the sensing capabilities of an electrochemical immunosensor employing polyaniline as a mediator species"]}]}],"canonical_facts":{"dc:creator":["Walsh, Padraic"],"dc:date":["2006"],"dc:date.issued":["2006"],"dc:description.abstract":["The aim of this thesis was to expand the sensing capabilities of a conducting polymer-based sensor platform developed in our laboratory. The platform itself is composed of a polyaniline - poly(vinylsulphonate) (PANIIPVS) modified screen-printed carbon paste electrode used as a substrate for the immobilisation of antibody, and the subsequent competition assays of protein analytes. Chapter 1 is a literature review encompassing the field of electrochemical immunosensing. chapter 3 outlines work towards developing real-time imrnunosensors to oestradiol and p60. This ork involved the synthesis of horseradish peroxidase (HRP) conjugates with oestradiol, and p60 or use in a competitive immunosensor, as well as immunoassay characterisation of the proteins, nd their application to the biosensor platform. Chapter 4 outlines efforts to address sub-monolayer protein immobilisation issues to the PANIIPVS matrix by optimising experimental parameters overning the electrostatic irnmobilisation process. It also details experiments probing icroperoxidase-11 as an alternative labeling enzyme to HRP. Chapter 5 deals with the investigation of a novel surfactant-based strategy to reduce non-specific interactions between species present in the bulk solution, and the PANIIPVS matrix. This research highlights the need for developing the technology for real-time electrochemical immunosensing, and examines some of the critical issues involved."],"dc:format":["application/pdf"],"dc:identifier.uri":["https://doras.dcu.ie/17000/1/padraic-walsh_SC.pdf"],"dc:language":["en"],"dc:publisher.institution":["Dublin City University"],"dc:relation.isreferencedby":["https://doras.dcu.ie/17000/"],"dc:subject":["Chemical detectors"],"dc:title":["Investigations into the sensing capabilities of an electrochemical immunosensor employing polyaniline as a mediator species"],"dc:type":["Thesis"],"dc:type.qualificationlevel":["masters"],"dc:type.qualificationname":["msc"]},"updated_at":"2026-07-24T02:00:50Z"}