Osseointegration of a Cementless Total Knee Arthroplasty in a Murine Model of Glucocorticoid Induced Osteoporosis
type: CreativeWork
description: Glucocorticoid-Induced Osteoporosis (GIOP) is the second largest cause of osteoporosis worldwide. Glucocorticoid-Induced osteoporosis is rapidly, driven principally by deficient osteoblast activity (i.e., decreased bone formation) rather than increased osteoclastic activity. Glucocorticoid-type steroids (GC) are used long-term typically for inflammatory diseases such as rheumatoid arthritis, lupus, multiple sclerosis, and other autoimmune disorders. Inflammatory arthritis often leads to joint replacement at relatively young ages. However, patients who are on GC treatment appear to have poorer outcomes for total joint arthroplasty compared to healthy patients. This is thought to be due to the anti-anabolic effects of GCs and impaired bone healing and osseous integration. The precise reasons for this are not well understood, nor are the potential strategies for intervention to improve these outcomes clear. Experimental animal models are critical to understanding this problem and informing as to how treatments might be used to improve osseointegration in the face of GC treatment. In the current studies, we used an established cementless murine knee arthroplasty model to better understand how GCs impact bone regeneration, osseointegration and biomechanical strength. The mouse model used in this study consists of a laser sintered titanium beaded tibial component (2mm long, 1mm diameter) from which biomechanical pullout strength and bone ingrowth can be readily measured. The goal of the current study was to assess GCs and implant osseointegration and biomechanical strength after treatment. Skeletally mature female C57BL/6 (4-month-old, n=28) were assigned to either GC or placebo treatment groups. Mice received a 60-day slow-release prednisolone pellet (2.1mg/kg/day) or placebo (vehicle) pellet, placed subcutaneously. Four weeks later, each mouse received a custom-designed cementless tibial implant surgically placed in the right knee and mice were allowed 4 weeks to heal. …
abstract: Glucocorticoid-Induced Osteoporosis (GIOP) is the second largest cause of osteoporosis worldwide. Glucocorticoid-Induced osteoporosis is rapidly, driven principally by deficient osteoblast activity (i.e., decreased bone formation) rather than increased osteoclastic activity. Glucocorticoid-type steroids (GC) are used long-term typically for inflammatory diseases such as rheumatoid arthritis, lupus, multiple sclerosis, and other autoimmune disorders. Inflammatory arthritis often leads to joint replacement at relatively young ages. However, patients who are on GC treatment appear to have poorer outcomes for total joint arthroplasty compared to healthy patients. This is thought to be due to the anti-anabolic effects of GCs and impaired bone healing and osseous integration. The precise reasons for this are not well understood, nor are the potential strategies for intervention to improve these outcomes clear. Experimental animal models are critical to understanding this problem and informing as to how treatments might be used to improve osseointegration in the face of GC treatment. In the current studies, we used an established cementless murine knee arthroplasty model to better understand how GCs impact bone regeneration, osseointegration and biomechanical strength. The mouse model used in this study consists of a laser sintered titanium beaded tibial component (2mm long, 1mm diameter) from which biomechanical pullout strength and bone ingrowth can be readily measured. The goal of the current study was to assess GCs and implant osseointegration and biomechanical strength after treatment. Skeletally mature female C57BL/6 (4-month-old, n=28) were assigned to either GC or placebo treatment groups. Mice received a 60-day slow-release prednisolone pellet (2.1mg/kg/day) or placebo (vehicle) pellet, placed subcutaneously. Four weeks later, each mouse received a custom-designed cementless tibial implant surgically placed in the right knee and mice were allowed 4 weeks to heal. …
author: Baral, Elexis C
keywords: Glucocorticoid, Osseointegration, Prednisolone, Total Knee Arthroplasty, Cementless, Biomedical Engineering and Bioengineering
date published: 2022-01-01T08:00:00Z
date modified: 2026-07-24T01:57:59Z
provider: City University of New York - City College
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: City University of New York - City College