Antibodies have long played a vital role in nuclear medicine for both the diagnosis and therapy of various malignancies. The role and development of antibodies in nuclear medicine can be broadly separated into three different categories: 1) bioconjugation strategies, 2) immunoPET imaging, and 3) radioimmunotherapy. This dissertation will attempt to comprehensively cover each of these categories through a series of studies, protocols, and reviews. For the bioconjugation strategies, we will describe the development of a novel site-selective bioconjugation strategy using an innovative lysine-targeting reagent, PFP-bisN3, to prepare [89Zr]Zr-SSKDFO-pertuzumab for visualizing HER2+ breast cancer. Further, we introduce a new mAb with a catalytic domain that allows for the site-selective bioconjugation of chelators to yield [177Lu]Lu-CHX-A”-DTPA-PODS-HER2DVD for targeting HER2+ breast cancer. For immunoPET, several chapters of this dissertation are dedicated to discussing the development of a CD133- targeting monoclonal antibody — [89Zr]Zr-DFO-aCD133 — majorly focusing on immunoPET imaging of small cell lung cancer in traditional and advanced tumor murine models. We have recently begun to explore the therapeutic efficacy of this immunoconjugate as well using the 177Lu labeled derivative, [177Lu]Lu-DTPA-A”-CHX-aCD133. As for radioimmunotherapy, we describe creating a HER2-targeting nanobody with a residualizing prosthetic group for radiohalogenation to eventually be used for therapy of HER2+ malignancies. And lastly, we report a protocol for harnessing the inverse electron demand Diels-Alder (IEDDA) click reaction for diagnostic and therapeutic pretargeting; an overall introduction to antibodies in nuclear medicine; and a review based on the role of click chemistry and its advances in recent years.
","abstract_html":"<p>Antibodies have long played a vital role in nuclear medicine for both the diagnosis and therapy of various malignancies. The role and development of antibodies in nuclear medicine can be broadly separated into three different categories: 1) bioconjugation strategies, 2) immunoPET imaging, and 3) radioimmunotherapy. This dissertation will attempt to comprehensively cover each of these categories through a series of studies, protocols, and reviews. For the bioconjugation strategies, we will describe the development of a novel site-selective bioconjugation strategy using an innovative lysine-targeting reagent, PFP-bisN<sub>3</sub>, to prepare [<sup>89</sup>Zr]Zr-<sup>SSK</sup>DFO-pertuzumab for visualizing HER2+ breast cancer. Further, we introduce a new mAb with a catalytic domain that allows for the site-selective bioconjugation of chelators to yield [<sup>177</sup>Lu]Lu-CHX-A”-DTPA-PODS-HER2DVD for targeting HER2+ breast cancer. For immunoPET, several chapters of this dissertation are dedicated to discussing the development of a CD133- targeting monoclonal antibody — [<sup>89</sup>Zr]Zr-DFO-aCD133 — majorly focusing on immunoPET imaging of small cell lung cancer in traditional and advanced tumor murine models. We have recently begun to explore the therapeutic efficacy of this immunoconjugate as well using the <sup>177</sup>Lu labeled derivative, [<sup>177</sup>Lu]Lu-DTPA-A”-CHX-aCD133. As for radioimmunotherapy, we describe creating a HER2-targeting nanobody with a residualizing prosthetic group for radiohalogenation to eventually be used for therapy of HER2+ malignancies. And lastly, we report a protocol for harnessing the inverse electron demand Diels-Alder (IEDDA) click reaction for diagnostic and therapeutic pretargeting; an overall introduction to antibodies in nuclear medicine; and a review based on the role of click chemistry and its advances in recent years.</p>","abstract_has_math":false,"creators":["Sarrett, Samantha M"],"institution":"The Graduate School and University Center of The City University of New York","degree_name":"Doctor of Philosophy","degree_level":"Doctoral","degree_discipline":"Biochemistry","degree_department":null,"school":null,"contributors":[],"advisors":["Brian Zeglis"],"committee_chairs":[],"committee_members":["Thomas Reiner","Melissa Deri","Jill Bargonetti","Jennifer Shusterman"],"year":2023,"date_issued":"2023-06-01T07:00:00Z","date_published":"2023-06-01T07:00:00Z","updated_at":"2026-07-24T01:59:38Z","subjects":["Biochemistry","Medicinal-Pharmaceutical Chemistry","Radiochemistry","radioimmunoconjugate","positron emission tomography","radioimmunotherapy","monoclonal antibody","radiopharmaceutical","nuclear medicine"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://academicworks.cuny.edu/gc_etds/5271","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Brian Zeglis"]},{"key":"dc:contributor.committeemember","label":"Committee Member","values":["Thomas Reiner","Melissa Deri","Jill Bargonetti","Jennifer Shusterman"]},{"key":"dc:creator","label":"Author","values":["Sarrett, Samantha M"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2023-06-01T07:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biochemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Doctoral"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Doctor of Philosophy"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["The Graduate School and University Center of The City University of New York"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biochemistry","Medicinal-Pharmaceutical Chemistry","Radiochemistry","radioimmunoconjugate","positron emission tomography","radioimmunotherapy","monoclonal antibody","radiopharmaceutical","nuclear medicine"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://academicworks.cuny.edu/gc_etds/5271"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Antibodies have long played a vital role in nuclear medicine for both the diagnosis and therapy of various malignancies. The role and development of antibodies in nuclear medicine can be broadly separated into three different categories: 1) bioconjugation strategies, 2) immunoPET imaging, and 3) radioimmunotherapy. This dissertation will attempt to comprehensively cover each of these categories through a series of studies, protocols, and reviews. For the bioconjugation strategies, we will describe the development of a novel site-selective bioconjugation strategy using an innovative lysine-targeting reagent, PFP-bisN3, to prepare [89Zr]Zr-SSKDFO-pertuzumab for visualizing HER2+ breast cancer. Further, we introduce a new mAb with a catalytic domain that allows for the site-selective bioconjugation of chelators to yield [177Lu]Lu-CHX-A”-DTPA-PODS-HER2DVD for targeting HER2+ breast cancer. For immunoPET, several chapters of this dissertation are dedicated to discussing the development of a CD133- targeting monoclonal antibody — [89Zr]Zr-DFO-aCD133 — majorly focusing on immunoPET imaging of small cell lung cancer in traditional and advanced tumor murine models. We have recently begun to explore the therapeutic efficacy of this immunoconjugate as well using the 177Lu labeled derivative, [177Lu]Lu-DTPA-A”-CHX-aCD133. As for radioimmunotherapy, we describe creating a HER2-targeting nanobody with a residualizing prosthetic group for radiohalogenation to eventually be used for therapy of HER2+ malignancies. And lastly, we report a protocol for harnessing the inverse electron demand Diels-Alder (IEDDA) click reaction for diagnostic and therapeutic pretargeting; an overall introduction to antibodies in nuclear medicine; and a review based on the role of click chemistry and its advances in recent years.
"]},{"key":"dc:title","label":"Title","values":["The Development of Novel Radioimmunoconjugates for the PET Imaging and Radioimmunotherapy of Cancer"]}]}],"canonical_facts":{"dc:contributor.advisor":["Brian Zeglis"],"dc:contributor.committeemember":["Thomas Reiner","Melissa Deri","Jill Bargonetti","Jennifer Shusterman"],"dc:creator":["Sarrett, Samantha M"],"dc:date.available":["2023-06-01T07:00:00Z"],"dc:description.abstract":["Antibodies have long played a vital role in nuclear medicine for both the diagnosis and therapy of various malignancies. The role and development of antibodies in nuclear medicine can be broadly separated into three different categories: 1) bioconjugation strategies, 2) immunoPET imaging, and 3) radioimmunotherapy. This dissertation will attempt to comprehensively cover each of these categories through a series of studies, protocols, and reviews. For the bioconjugation strategies, we will describe the development of a novel site-selective bioconjugation strategy using an innovative lysine-targeting reagent, PFP-bisN3, to prepare [89Zr]Zr-SSKDFO-pertuzumab for visualizing HER2+ breast cancer. Further, we introduce a new mAb with a catalytic domain that allows for the site-selective bioconjugation of chelators to yield [177Lu]Lu-CHX-A”-DTPA-PODS-HER2DVD for targeting HER2+ breast cancer. For immunoPET, several chapters of this dissertation are dedicated to discussing the development of a CD133- targeting monoclonal antibody — [89Zr]Zr-DFO-aCD133 — majorly focusing on immunoPET imaging of small cell lung cancer in traditional and advanced tumor murine models. We have recently begun to explore the therapeutic efficacy of this immunoconjugate as well using the 177Lu labeled derivative, [177Lu]Lu-DTPA-A”-CHX-aCD133. As for radioimmunotherapy, we describe creating a HER2-targeting nanobody with a residualizing prosthetic group for radiohalogenation to eventually be used for therapy of HER2+ malignancies. And lastly, we report a protocol for harnessing the inverse electron demand Diels-Alder (IEDDA) click reaction for diagnostic and therapeutic pretargeting; an overall introduction to antibodies in nuclear medicine; and a review based on the role of click chemistry and its advances in recent years.
"],"dc:identifier":["https://academicworks.cuny.edu/gc_etds/5271"],"dc:subject":["Biochemistry","Medicinal-Pharmaceutical Chemistry","Radiochemistry","radioimmunoconjugate","positron emission tomography","radioimmunotherapy","monoclonal antibody","radiopharmaceutical","nuclear medicine"],"dc:title":["The Development of Novel Radioimmunoconjugates for the PET Imaging and Radioimmunotherapy of Cancer"],"thesis:degree_discipline":["Biochemistry"],"thesis:degree_level":["Doctoral"],"thesis:degree_name":["Doctor of Philosophy"],"thesis:institution_name":["The Graduate School and University Center of The City University of New York"]},"updated_at":"2026-07-24T01:59:38Z"}