Novel sustained release formulation of leveodopa-dendrimer conjugate
type: CreativeWork
description: Fibrillation of alpha synuclein (AS) leading to the formation of Lew body has been ubiquitously reported in many geriatrics disorders such as Parkinson's disease (PD), Alzheimer's disease, and other neurodegenerative disorders. Therefore, any substance inhibiting or reducing fibrillation of alpha synuclein is expected to potentially alleviate these disease symptoms. Levodopa is the current drug of choice for the treatment of PD. The effectiveness of any investigative PD drug is compared with that of levodopa. However, its dose needs to increase with chronic use and there are some side effects like nausea and vomiting are associated with the metabolic products of levodopa such as3-ortho methyl dopa and dopamine. It has also been reported that the fluctuating levels of levodopa is responsible for the wearing off effect. The best way to solve these problems was to deliver a sustained amount of levodopa to the brain so that a constant level of dopamine is maintained at the dopamine receptor sites. Therefore, in this study a novel sustained release formulation of levodopa was prepared which efficacy was evaluated by measuring the extent/amount of AS fibrillation. In vitro fibrillation of AS was induced by shaking its solution (37OC, pH=7.4) at 600 rpm. The fibrillation of AS was characterized quantitatively by using Thioflavin t-based fluorescence and Fourier Transform Infrared (FTIR) spectroscopy, and qualitatively by using light microscopy. Thioflavin t assay was chosen due its property to specifically bind to amyloid fibrils and as a result exhibits a higher fluorescence at excitation and emission wavelengths of 440 nm and 490 nm respectively. FTIR technique is also a sensitive method to evaluate formation of alpha synuclein fibrils. There is a characteristic increase in the secondary structure of alpha synuclein due to a change in the arrangement of AS structure from natively unstructured to a highly oriv dered structure after fibrillation. Light microscopy was used …
abstract: Fibrillation of alpha synuclein (AS) leading to the formation of Lew body has been ubiquitously reported in many geriatrics disorders such as Parkinson's disease (PD), Alzheimer's disease, and other neurodegenerative disorders. Therefore, any substance inhibiting or reducing fibrillation of alpha synuclein is expected to potentially alleviate these disease symptoms. Levodopa is the current drug of choice for the treatment of PD. The effectiveness of any investigative PD drug is compared with that of levodopa. However, its dose needs to increase with chronic use and there are some side effects like nausea and vomiting are associated with the metabolic products of levodopa such as3-ortho methyl dopa and dopamine. It has also been reported that the fluctuating levels of levodopa is responsible for the wearing off effect. The best way to solve these problems was to deliver a sustained amount of levodopa to the brain so that a constant level of dopamine is maintained at the dopamine receptor sites. Therefore, in this study a novel sustained release formulation of levodopa was prepared which efficacy was evaluated by measuring the extent/amount of AS fibrillation. In vitro fibrillation of AS was induced by shaking its solution (37OC, pH=7.4) at 600 rpm. The fibrillation of AS was characterized quantitatively by using Thioflavin t-based fluorescence and Fourier Transform Infrared (FTIR) spectroscopy, and qualitatively by using light microscopy. Thioflavin t assay was chosen due its property to specifically bind to amyloid fibrils and as a result exhibits a higher fluorescence at excitation and emission wavelengths of 440 nm and 490 nm respectively. FTIR technique is also a sensitive method to evaluate formation of alpha synuclein fibrils. There is a characteristic increase in the secondary structure of alpha synuclein due to a change in the arrangement of AS structure from natively unstructured to a highly oriv dered structure after fibrillation. Light microscopy was used …
author: Mulla, Nihal S.
language: en_US
date published: 2011-07-20
date modified: 2026-07-24T01:50:02Z
provider: Creighton University
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Creighton University

PropertyValue
property ID: Handle
value: http://hdl.handle.net/10504/18918