{"id":{"repo_id":"creighton","oai_identifier":"oai:cdr.creighton.edu:10504/118424"},"canonical_url":"https://search.dev.ndltd.org/etd/creighton/oai:cdr.creighton.edu:10504/118424","repository":{"repo_id":"creighton","name":"Creighton University","base_url":"https://cdr.creighton.edu/server/oai/request"},"display":{"title":"Enzyme Mediated Gentamicin Resistance Among Gram-Negative Clinical Isolates","abstract":"Gentamicin is a broad-spectrum, bactericidal antibiotic of the aminoglycoside family. It has been shown through extensive evaluation to be effective in the treatment of serious gram- negative bacillary infections (1-5). Gentamicin is one of the few antimicrobial agents available for the treatment of life- threatening infections due to Pseudomonas aeruginosa (114-116), an organism of increasing etiological significance in opportunistic infections (6). | Perhaps as a result of the use of gentamicin, and its significant misuse, the incidence of resistance to gentamicin among clinical isolates (of formerly susceptible bacteria) is on the rise (7-12). Resistance to the aminoglycosides is believed to be due to three basic mechanisms (13,14), The best characterized mechanism is the enzyme-mediated inactivation of antibiotic activity. To date eight different enzymes have been described (15). The three primary classes of enzymes (based upon the nature of the group they transfer to the aminoglycoside molecule) are: (a) an adenylylating enzyme (adenylyltrans-ferase) (13), (b) an acetylating enzyme (acetyltransferase) (13), and (c) a phosphorylating enzyme (phosphotransferase) (13).","abstract_html":"Gentamicin is a broad-spectrum, bactericidal antibiotic of the aminoglycoside family. It has been shown through extensive evaluation to be effective in the treatment of serious gram- negative bacillary infections (1-5). Gentamicin is one of the few antimicrobial agents available for the treatment of life- threatening infections due to Pseudomonas aeruginosa (114-116), an organism of increasing etiological significance in opportunistic infections (6). | Perhaps as a result of the use of gentamicin, and its significant misuse, the incidence of resistance to gentamicin among clinical isolates (of formerly susceptible bacteria) is on the rise (7-12). Resistance to the aminoglycosides is believed to be due to three basic mechanisms (13,14), The best characterized mechanism is the enzyme-mediated inactivation of antibiotic activity. To date eight different enzymes have been described (15). The three primary classes of enzymes (based upon the nature of the group they transfer to the aminoglycoside molecule) are: (a) an adenylylating enzyme (adenylyltrans-ferase) (13), (b) an acetylating enzyme (acetyltransferase) (13), and (c) a phosphorylating enzyme (phosphotransferase) (13).","abstract_has_math":false,"creators":["Weiss, Ronald Leslie"],"institution":"Creighton University","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Walker, Cynthia A."],"committee_chairs":[],"committee_members":[],"year":1976,"date_issued":"1976","date_published":"1976","updated_at":"2026-07-24T01:51:41Z","subjects":["Microbiology"],"languages":["en_US"],"rights":["A non-exclusive distribution right is granted to Creighton University and to ProQuest following the publishing model selected above."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10504/118424","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Walker, Cynthia A."]},{"key":"dc:creator","label":"Author","values":["Weiss, Ronald Leslie"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2018-06-19T17:02:45Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2018-06-19T17:02:45Z"]},{"key":"dc:date.issued","label":"Date","values":["1976"]},{"key":"dc:publisher","label":"Institution","values":["Creighton University"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Microbiology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en_US"]},{"key":"dc:rights","label":"Dc Rights","values":["A non-exclusive distribution right is granted to Creighton University and to ProQuest following the publishing model selected above."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/10504/118424"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Gentamicin is a broad-spectrum, bactericidal antibiotic of the aminoglycoside family. It has been shown through extensive evaluation to be effective in the treatment of serious gram- negative bacillary infections (1-5). Gentamicin is one of the few antimicrobial agents available for the treatment of life- threatening infections due to Pseudomonas aeruginosa (114-116), an organism of increasing etiological significance in opportunistic infections (6). | Perhaps as a result of the use of gentamicin, and its significant misuse, the incidence of resistance to gentamicin among clinical isolates (of formerly susceptible bacteria) is on the rise (7-12). Resistance to the aminoglycosides is believed to be due to three basic mechanisms (13,14), The best characterized mechanism is the enzyme-mediated inactivation of antibiotic activity. To date eight different enzymes have been described (15). The three primary classes of enzymes (based upon the nature of the group they transfer to the aminoglycoside molecule) are: (a) an adenylylating enzyme (adenylyltrans-ferase) (13), (b) an acetylating enzyme (acetyltransferase) (13), and (c) a phosphorylating enzyme (phosphotransferase) (13)."]},{"key":"dc:title","label":"Title","values":["Enzyme Mediated Gentamicin Resistance Among Gram-Negative Clinical Isolates"]}]}],"canonical_facts":{"dc:contributor.advisor":["Walker, Cynthia A."],"dc:creator":["Weiss, Ronald Leslie"],"dc:date.accessioned":["2018-06-19T17:02:45Z"],"dc:date.available":["2018-06-19T17:02:45Z"],"dc:date.issued":["1976"],"dc:description.abstract":["Gentamicin is a broad-spectrum, bactericidal antibiotic of the aminoglycoside family. It has been shown through extensive evaluation to be effective in the treatment of serious gram- negative bacillary infections (1-5). Gentamicin is one of the few antimicrobial agents available for the treatment of life- threatening infections due to Pseudomonas aeruginosa (114-116), an organism of increasing etiological significance in opportunistic infections (6). | Perhaps as a result of the use of gentamicin, and its significant misuse, the incidence of resistance to gentamicin among clinical isolates (of formerly susceptible bacteria) is on the rise (7-12). Resistance to the aminoglycosides is believed to be due to three basic mechanisms (13,14), The best characterized mechanism is the enzyme-mediated inactivation of antibiotic activity. To date eight different enzymes have been described (15). The three primary classes of enzymes (based upon the nature of the group they transfer to the aminoglycoside molecule) are: (a) an adenylylating enzyme (adenylyltrans-ferase) (13), (b) an acetylating enzyme (acetyltransferase) (13), and (c) a phosphorylating enzyme (phosphotransferase) (13)."],"dc:identifier.uri":["http://hdl.handle.net/10504/118424"],"dc:language.iso":["en_US"],"dc:publisher":["Creighton University"],"dc:rights":["A non-exclusive distribution right is granted to Creighton University and to ProQuest following the publishing model selected above."],"dc:subject":["Microbiology"],"dc:title":["Enzyme Mediated Gentamicin Resistance Among Gram-Negative Clinical Isolates"],"dc:type":["Thesis"]},"updated_at":"2026-07-24T01:51:41Z"}