{"id":{"repo_id":"creighton","oai_identifier":"oai:cdr.creighton.edu:10504/118281"},"canonical_url":"https://search.dev.ndltd.org/etd/creighton/oai:cdr.creighton.edu:10504/118281","repository":{"repo_id":"creighton","name":"Creighton University","base_url":"https://cdr.creighton.edu/server/oai/request"},"display":{"title":"A Study of the Action of Prostaglandin F2a on the Guinea-Pig Isolated Trachea","abstract":"The action of prostaglandin F2a (PGF2a)1 on guinea-pig isolated trachea has been studied. Special emphasis was placed on considerations of the possible beta-adrenergic blocking activities of this essential fatty-acid derivative. | Experiments were performed using a 20 ml tissue bath and a modified phosphate-Tyrode solution. Measurements were made by an isotonic myograph and displayed on a Physiograph recorder. | Cumulative log dose-response curves to isoproterenol before and after either PGF2a or a beta blocker (MJ 1999) provided data for calculating and plotting both isoboles and Lineweaver-Burk curves. These, calculations indicated that PGF2a is not inhibitory to the action of isoproterenol on guinea-pig trachea in vitro. | Further experiments demonstrated that PGF2a possesses a weak inhibitory activity on methacholine-induced tracheal tone. That this inhibitory activity is not mediated through the beta- adrenergic receptor was shown in experiments where beta blocker (MJ 1999) failed to inhibit the activity of PGF2a. Inhibition of methacholine-induced tone by PGF2a was further differentiated from that produced by isoproterenol on the basis of observations that PGF2a-induced inhibition was incomplete, even at the highest concentrations.","abstract_html":"The action of prostaglandin F2a (PGF2a)1 on guinea-pig isolated trachea has been studied. Special emphasis was placed on considerations of the possible beta-adrenergic blocking activities of this essential fatty-acid derivative. | Experiments were performed using a 20 ml tissue bath and a modified phosphate-Tyrode solution. Measurements were made by an isotonic myograph and displayed on a Physiograph recorder. | Cumulative log dose-response curves to isoproterenol before and after either PGF2a or a beta blocker (MJ 1999) provided data for calculating and plotting both isoboles and Lineweaver-Burk curves. These, calculations indicated that PGF2a is not inhibitory to the action of isoproterenol on guinea-pig trachea in vitro. | Further experiments demonstrated that PGF2a possesses a weak inhibitory activity on methacholine-induced tracheal tone. That this inhibitory activity is not mediated through the beta- adrenergic receptor was shown in experiments where beta blocker (MJ 1999) failed to inhibit the activity of PGF2a. Inhibition of methacholine-induced tone by PGF2a was further differentiated from that produced by isoproterenol on the basis of observations that PGF2a-induced inhibition was incomplete, even at the highest concentrations.","abstract_has_math":false,"creators":["Adolphson, Richard Louis"],"institution":"Creighton University","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Townley, Robert G."],"committee_chairs":[],"committee_members":[],"year":1970,"date_issued":"1970","date_published":"1970","updated_at":"2026-07-24T01:50:46Z","subjects":[],"languages":["en_US"],"rights":["A non-exclusive distribution right is granted to Creighton University and to ProQuest following the publishing model selected above."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10504/118281","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Townley, Robert G."]},{"key":"dc:creator","label":"Author","values":["Adolphson, Richard Louis"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2018-06-01T16:50:03Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2018-06-01T16:50:03Z"]},{"key":"dc:date.issued","label":"Date","values":["1970"]},{"key":"dc:publisher","label":"Institution","values":["Creighton University"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en_US"]},{"key":"dc:rights","label":"Dc Rights","values":["A non-exclusive distribution right is granted to Creighton University and to ProQuest following the publishing model selected above."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/10504/118281"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The action of prostaglandin F2a (PGF2a)1 on guinea-pig isolated trachea has been studied. Special emphasis was placed on considerations of the possible beta-adrenergic blocking activities of this essential fatty-acid derivative. | Experiments were performed using a 20 ml tissue bath and a modified phosphate-Tyrode solution. Measurements were made by an isotonic myograph and displayed on a Physiograph recorder. | Cumulative log dose-response curves to isoproterenol before and after either PGF2a or a beta blocker (MJ 1999) provided data for calculating and plotting both isoboles and Lineweaver-Burk curves. These, calculations indicated that PGF2a is not inhibitory to the action of isoproterenol on guinea-pig trachea in vitro. | Further experiments demonstrated that PGF2a possesses a weak inhibitory activity on methacholine-induced tracheal tone. That this inhibitory activity is not mediated through the beta- adrenergic receptor was shown in experiments where beta blocker (MJ 1999) failed to inhibit the activity of PGF2a. Inhibition of methacholine-induced tone by PGF2a was further differentiated from that produced by isoproterenol on the basis of observations that PGF2a-induced inhibition was incomplete, even at the highest concentrations."]},{"key":"dc:title","label":"Title","values":["A Study of the Action of Prostaglandin F2a on the Guinea-Pig Isolated Trachea"]}]}],"canonical_facts":{"dc:contributor.advisor":["Townley, Robert G."],"dc:creator":["Adolphson, Richard Louis"],"dc:date.accessioned":["2018-06-01T16:50:03Z"],"dc:date.available":["2018-06-01T16:50:03Z"],"dc:date.issued":["1970"],"dc:description.abstract":["The action of prostaglandin F2a (PGF2a)1 on guinea-pig isolated trachea has been studied. Special emphasis was placed on considerations of the possible beta-adrenergic blocking activities of this essential fatty-acid derivative. | Experiments were performed using a 20 ml tissue bath and a modified phosphate-Tyrode solution. Measurements were made by an isotonic myograph and displayed on a Physiograph recorder. | Cumulative log dose-response curves to isoproterenol before and after either PGF2a or a beta blocker (MJ 1999) provided data for calculating and plotting both isoboles and Lineweaver-Burk curves. These, calculations indicated that PGF2a is not inhibitory to the action of isoproterenol on guinea-pig trachea in vitro. | Further experiments demonstrated that PGF2a possesses a weak inhibitory activity on methacholine-induced tracheal tone. That this inhibitory activity is not mediated through the beta- adrenergic receptor was shown in experiments where beta blocker (MJ 1999) failed to inhibit the activity of PGF2a. Inhibition of methacholine-induced tone by PGF2a was further differentiated from that produced by isoproterenol on the basis of observations that PGF2a-induced inhibition was incomplete, even at the highest concentrations."],"dc:identifier.uri":["http://hdl.handle.net/10504/118281"],"dc:language.iso":["en_US"],"dc:publisher":["Creighton University"],"dc:rights":["A non-exclusive distribution right is granted to Creighton University and to ProQuest following the publishing model selected above."],"dc:title":["A Study of the Action of Prostaglandin F2a on the Guinea-Pig Isolated Trachea"],"dc:type":["Thesis"]},"updated_at":"2026-07-24T01:50:46Z"}