{"id":{"repo_id":"creighton","oai_identifier":"oai:cdr.creighton.edu:10504/117885"},"canonical_url":"https://search.dev.ndltd.org/etd/creighton/oai:cdr.creighton.edu:10504/117885","repository":{"repo_id":"creighton","name":"Creighton University","base_url":"https://cdr.creighton.edu/server/oai/request"},"display":{"title":"Regulation of cataractogenesis in cultured bovine lenses by hydrogen sulfide donors","abstract":"There is no drug for cataracts, a leading cause of blindness. However, L-cysteine reportedly mitigates cataractogenesis, in vitro and in vivo. Here, we sought to standardize a new method for screening cataractogenesis and assess the effect of hydrogen sulfide (H2S) on cataractogenesis. Bovine lenses were cultured in DMEM with AA (positive control) or compounds [L-cysteine, diallyl trisulfide (DATS) and GYY 4137 (GYY)] in presence or absence of hydrogen peroxide (H2O2). Lens light transmittance and optical clarity were assessed and confirmed with biochemical assays. One-way Analysis of variance (ANOVA) and two-Way ANOVA with p<0.05 were considered significant. Cultured lenses showed opacity (p<0.0001) after 120 hours and was accelerated by H2O2 (50mM). Lenticular for total glutathione content (GSH) [46.08%, 82.56%] and total superoxide dismutase (SOD) activity [42.02%, 86.59%] decreased (p<0.0001) in untreated and H2O2- treated lenses respectively. AA (10 mM) attenuated time-dependent loss of lens transparency (p<0.0001) for 24 hours; while AA (3 mM) attenuated H2O2-induced opacity (p<0.0001) up to 120 hours. L-cysteine (10-6 M & 10-5 M), DATS (10-7 M & 10-6 M) and GYY (10-7 M & 10-6 M) mitigated (p<0.0001) loss of lens transparency up to 120 hours superior to AA (10 mM). L-cysteine (10-6 M), DATS (10-6 M) and GYY (10-7 M) reversed (p<0.0001) time-dependent decrease in GSH content and SOD activity by [76.58%, 7.42%], [69.9%, 3.32%] and [80.52%, 19.31%], respectively after 120 hours. Increased (p<0.01) cytotoxicity of 17.16% was noted for DATS (10-6 M) after 24 hours of incubation. L-cysteine (10-6 M to 10-4 M) & DATS (10-6 M & 10-4 M) reversed H2O2-induced opacity up to 120 hours greater than AA (3 mM). L-cysteine (10-4 M), DATS (10-4 M) and GYY (10-7M) reversed (p<0.0001) H2O2-induced decrease in GSH content and SOD activity by [74.67%, 161.1%], [121.48%, 109.78%] and [158.51%, 194.89%] respectively, after 120 hours. H2O2-induced bovine lens epithelial cells cytotoxicity was mitigated (p<0.0001) by 27.7% (L-cysteine [10-4 M]), 33.88% (DATS [10-4 M]) and 36.19% (GYY [10-7M]) after 24 hours. Measurement of cultured lens transmittance is a viable method for screening cataractogenesis. H2S protected lenses from time-dependent and H2O2-induced opacity, possibly due to its antioxidant property.","abstract_html":"There is no drug for cataracts, a leading cause of blindness. However, L-cysteine reportedly mitigates cataractogenesis, in vitro and in vivo. Here, we sought to standardize a new method for screening cataractogenesis and assess the effect of hydrogen sulfide (H2S) on cataractogenesis. Bovine lenses were cultured in DMEM with AA (positive control) or compounds [L-cysteine, diallyl trisulfide (DATS) and GYY 4137 (GYY)] in presence or absence of hydrogen peroxide (H2O2). Lens light transmittance and optical clarity were assessed and confirmed with biochemical assays. One-way Analysis of variance (ANOVA) and two-Way ANOVA with p&lt;0.05 were considered significant. Cultured lenses showed opacity (p&lt;0.0001) after 120 hours and was accelerated by H2O2 (50mM). Lenticular for total glutathione content (GSH) [46.08%, 82.56%] and total superoxide dismutase (SOD) activity [42.02%, 86.59%] decreased (p&lt;0.0001) in untreated and H2O2- treated lenses respectively. AA (10 mM) attenuated time-dependent loss of lens transparency (p&lt;0.0001) for 24 hours; while AA (3 mM) attenuated H2O2-induced opacity (p&lt;0.0001) up to 120 hours. L-cysteine (10-6 M &amp; 10-5 M), DATS (10-7 M &amp; 10-6 M) and GYY (10-7 M &amp; 10-6 M) mitigated (p&lt;0.0001) loss of lens transparency up to 120 hours superior to AA (10 mM). L-cysteine (10-6 M), DATS (10-6 M) and GYY (10-7 M) reversed (p&lt;0.0001) time-dependent decrease in GSH content and SOD activity by [76.58%, 7.42%], [69.9%, 3.32%] and [80.52%, 19.31%], respectively after 120 hours. Increased (p&lt;0.01) cytotoxicity of 17.16% was noted for DATS (10-6 M) after 24 hours of incubation. L-cysteine (10-6 M to 10-4 M) &amp; DATS (10-6 M &amp; 10-4 M) reversed H2O2-induced opacity up to 120 hours greater than AA (3 mM). L-cysteine (10-4 M), DATS (10-4 M) and GYY (10-7M) reversed (p&lt;0.0001) H2O2-induced decrease in GSH content and SOD activity by [74.67%, 161.1%], [121.48%, 109.78%] and [158.51%, 194.89%] respectively, after 120 hours. H2O2-induced bovine lens epithelial cells cytotoxicity was mitigated (p&lt;0.0001) by 27.7% (L-cysteine [10-4 M]), 33.88% (DATS [10-4 M]) and 36.19% (GYY [10-7M]) after 24 hours. Measurement of cultured lens transmittance is a viable method for screening cataractogenesis. H2S protected lenses from time-dependent and H2O2-induced opacity, possibly due to its antioxidant property.","abstract_has_math":false,"creators":["Heruye, Segewkal Hawaze"],"institution":"Creighton University","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Opere, Catherine A."],"committee_chairs":[],"committee_members":[],"year":2018,"date_issued":"2018-05-10","date_published":"2018-05-10","updated_at":"2026-07-24T01:51:30Z","subjects":[],"languages":["en_US"],"rights":["Copyright is retained by the Author. A non-exclusive distribution right is granted to Creighton University and to ProQuest following the publishing model selected above."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10504/117885","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Opere, Catherine A."]},{"key":"dc:creator","label":"Author","values":["Heruye, Segewkal Hawaze"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2018-05-10T21:27:13Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2020-05-10T08:40:22Z"]},{"key":"dc:date.issued","label":"Date","values":["2018-05-10"]},{"key":"dc:publisher","label":"Institution","values":["Creighton University"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en_US"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright is retained by the Author. A non-exclusive distribution right is granted to Creighton University and to ProQuest following the publishing model selected above."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/10504/117885"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["There is no drug for cataracts, a leading cause of blindness. However, L-cysteine reportedly mitigates cataractogenesis, in vitro and in vivo. Here, we sought to standardize a new method for screening cataractogenesis and assess the effect of hydrogen sulfide (H2S) on cataractogenesis. Bovine lenses were cultured in DMEM with AA (positive control) or compounds [L-cysteine, diallyl trisulfide (DATS) and GYY 4137 (GYY)] in presence or absence of hydrogen peroxide (H2O2). Lens light transmittance and optical clarity were assessed and confirmed with biochemical assays. One-way Analysis of variance (ANOVA) and two-Way ANOVA with p<0.05 were considered significant. Cultured lenses showed opacity (p<0.0001) after 120 hours and was accelerated by H2O2 (50mM). Lenticular for total glutathione content (GSH) [46.08%, 82.56%] and total superoxide dismutase (SOD) activity [42.02%, 86.59%] decreased (p<0.0001) in untreated and H2O2- treated lenses respectively. AA (10 mM) attenuated time-dependent loss of lens transparency (p<0.0001) for 24 hours; while AA (3 mM) attenuated H2O2-induced opacity (p<0.0001) up to 120 hours. L-cysteine (10-6 M & 10-5 M), DATS (10-7 M & 10-6 M) and GYY (10-7 M & 10-6 M) mitigated (p<0.0001) loss of lens transparency up to 120 hours superior to AA (10 mM). L-cysteine (10-6 M), DATS (10-6 M) and GYY (10-7 M) reversed (p<0.0001) time-dependent decrease in GSH content and SOD activity by [76.58%, 7.42%], [69.9%, 3.32%] and [80.52%, 19.31%], respectively after 120 hours. Increased (p<0.01) cytotoxicity of 17.16% was noted for DATS (10-6 M) after 24 hours of incubation. L-cysteine (10-6 M to 10-4 M) & DATS (10-6 M & 10-4 M) reversed H2O2-induced opacity up to 120 hours greater than AA (3 mM). L-cysteine (10-4 M), DATS (10-4 M) and GYY (10-7M) reversed (p<0.0001) H2O2-induced decrease in GSH content and SOD activity by [74.67%, 161.1%], [121.48%, 109.78%] and [158.51%, 194.89%] respectively, after 120 hours. H2O2-induced bovine lens epithelial cells cytotoxicity was mitigated (p<0.0001) by 27.7% (L-cysteine [10-4 M]), 33.88% (DATS [10-4 M]) and 36.19% (GYY [10-7M]) after 24 hours. Measurement of cultured lens transmittance is a viable method for screening cataractogenesis. H2S protected lenses from time-dependent and H2O2-induced opacity, possibly due to its antioxidant property."]},{"key":"dc:title","label":"Title","values":["Regulation of cataractogenesis in cultured bovine lenses by hydrogen sulfide donors"]}]}],"canonical_facts":{"dc:contributor.advisor":["Opere, Catherine A."],"dc:creator":["Heruye, Segewkal Hawaze"],"dc:date.accessioned":["2018-05-10T21:27:13Z"],"dc:date.available":["2020-05-10T08:40:22Z"],"dc:date.issued":["2018-05-10"],"dc:description.abstract":["There is no drug for cataracts, a leading cause of blindness. However, L-cysteine reportedly mitigates cataractogenesis, in vitro and in vivo. Here, we sought to standardize a new method for screening cataractogenesis and assess the effect of hydrogen sulfide (H2S) on cataractogenesis. Bovine lenses were cultured in DMEM with AA (positive control) or compounds [L-cysteine, diallyl trisulfide (DATS) and GYY 4137 (GYY)] in presence or absence of hydrogen peroxide (H2O2). Lens light transmittance and optical clarity were assessed and confirmed with biochemical assays. One-way Analysis of variance (ANOVA) and two-Way ANOVA with p<0.05 were considered significant. Cultured lenses showed opacity (p<0.0001) after 120 hours and was accelerated by H2O2 (50mM). Lenticular for total glutathione content (GSH) [46.08%, 82.56%] and total superoxide dismutase (SOD) activity [42.02%, 86.59%] decreased (p<0.0001) in untreated and H2O2- treated lenses respectively. AA (10 mM) attenuated time-dependent loss of lens transparency (p<0.0001) for 24 hours; while AA (3 mM) attenuated H2O2-induced opacity (p<0.0001) up to 120 hours. L-cysteine (10-6 M & 10-5 M), DATS (10-7 M & 10-6 M) and GYY (10-7 M & 10-6 M) mitigated (p<0.0001) loss of lens transparency up to 120 hours superior to AA (10 mM). L-cysteine (10-6 M), DATS (10-6 M) and GYY (10-7 M) reversed (p<0.0001) time-dependent decrease in GSH content and SOD activity by [76.58%, 7.42%], [69.9%, 3.32%] and [80.52%, 19.31%], respectively after 120 hours. Increased (p<0.01) cytotoxicity of 17.16% was noted for DATS (10-6 M) after 24 hours of incubation. L-cysteine (10-6 M to 10-4 M) & DATS (10-6 M & 10-4 M) reversed H2O2-induced opacity up to 120 hours greater than AA (3 mM). L-cysteine (10-4 M), DATS (10-4 M) and GYY (10-7M) reversed (p<0.0001) H2O2-induced decrease in GSH content and SOD activity by [74.67%, 161.1%], [121.48%, 109.78%] and [158.51%, 194.89%] respectively, after 120 hours. H2O2-induced bovine lens epithelial cells cytotoxicity was mitigated (p<0.0001) by 27.7% (L-cysteine [10-4 M]), 33.88% (DATS [10-4 M]) and 36.19% (GYY [10-7M]) after 24 hours. Measurement of cultured lens transmittance is a viable method for screening cataractogenesis. H2S protected lenses from time-dependent and H2O2-induced opacity, possibly due to its antioxidant property."],"dc:identifier.uri":["http://hdl.handle.net/10504/117885"],"dc:language.iso":["en_US"],"dc:publisher":["Creighton University"],"dc:rights":["Copyright is retained by the Author. A non-exclusive distribution right is granted to Creighton University and to ProQuest following the publishing model selected above."],"dc:title":["Regulation of cataractogenesis in cultured bovine lenses by hydrogen sulfide donors"],"dc:type":["Thesis"]},"updated_at":"2026-07-24T01:51:30Z"}