{"id":{"repo_id":"cornell","oai_identifier":"oai:ecommons.cornell.edu:1813/114506"},"canonical_url":"https://search.dev.ndltd.org/etd/cornell/oai:ecommons.cornell.edu:1813/114506","repository":{"repo_id":"cornell","name":"Cornell University","base_url":"https://ecommons.cornell.edu/server/oai/request"},"display":{"title":"Breeding broccoli resilient to multi-abiotic stresses using genome editing","abstract":"Broccoli cultivars are sensitive to environmental stresses. Dramatic fluctuation in temperature and other weather conditions caused by climate change has negatively affected the growth and development of broccoli and reduced broccoli productivity and quality. Plant senescence is one of the most important consequences of all adverse environmental conditions or abiotic stresses. NAC-LIKE, ACTIVATED BY AP3/PI (NAP), a NAC (NAM, ATAF1/2, and CUC2) family transcription factor, is a key regulator of abiotic stress-induced senescence. Knocking BoNAP out via CRISPR/Cas9 genome editing in broccoli (Brassica oleracea var. italica) renders the broccoli lines more resistant to multiple abiotic stresses including drought, salt, extreme temperatures, strong UV radiation, and darkness phenotypically and physiologically. The research provides a novel strategy for breeding broccoli germplasm resilient to climate change.","abstract_html":"Broccoli cultivars are sensitive to environmental stresses. Dramatic fluctuation in temperature and other weather conditions caused by climate change has negatively affected the growth and development of broccoli and reduced broccoli productivity and quality. Plant senescence is one of the most important consequences of all adverse environmental conditions or abiotic stresses. NAC-LIKE, ACTIVATED BY AP3/PI (NAP), a NAC (NAM, ATAF1/2, and CUC2) family transcription factor, is a key regulator of abiotic stress-induced senescence. Knocking BoNAP out via CRISPR/Cas9 genome editing in broccoli (Brassica oleracea var. italica) renders the broccoli lines more resistant to multiple abiotic stresses including drought, salt, extreme temperatures, strong UV radiation, and darkness phenotypically and physiologically. The research provides a novel strategy for breeding broccoli germplasm resilient to climate change.","abstract_has_math":false,"creators":["Meng, Wei"],"institution":"Cornell University","degree_name":"M.S., Horticulture","degree_level":"Master of Science","degree_discipline":"Horticulture","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":["Griffiths, Phillip"],"year":2023,"date_issued":"2023-08","date_published":"2023-08","updated_at":"2026-07-24T01:49:02Z","subjects":["broccoli","abiotic stress","senescence","NAP","CRISPR/Cas9","resilience"],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier.doi","label":"DOI","values":["https://doi.org/10.7298/5c29-xh92"],"render_values":[{"text":"https://doi.org/10.7298/5c29-xh92","href":"https://doi.org/10.7298/5c29-xh92","code":true}]},{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["ProQuest Submission ID: 11728","ProQuest Publication ID: 30485200"],"render_values":[{"text":"ProQuest Submission ID: 11728","href":null,"code":true},{"text":"ProQuest Publication ID: 30485200","href":null,"code":true}]}]},"links":{"outbound_url":"https://hdl.handle.net/1813/114506","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.committeemember","label":"Committee Member","values":["Griffiths, Phillip"]},{"key":"dc:creator","label":"Author","values":["Meng, Wei"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2024-04-05T18:36:42Z"]},{"key":"dc:date.issued","label":"Date","values":["2023-08"]},{"key":"dc:type","label":"Dc Type","values":["dissertation or thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Horticulture"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Master of Science"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S., Horticulture"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["Cornell University"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["broccoli","abiotic stress","senescence","NAP","CRISPR/Cas9","resilience"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.doi","label":"DOI","values":["https://doi.org/10.7298/5c29-xh92"]},{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["ProQuest Submission ID: 11728","ProQuest Publication ID: 30485200"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://hdl.handle.net/1813/114506"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["142 pages"]},{"key":"dc:description.abstract","label":"Abstract","values":["Broccoli cultivars are sensitive to environmental stresses. Dramatic fluctuation in temperature and other weather conditions caused by climate change has negatively affected the growth and development of broccoli and reduced broccoli productivity and quality. Plant senescence is one of the most important consequences of all adverse environmental conditions or abiotic stresses. NAC-LIKE, ACTIVATED BY AP3/PI (NAP), a NAC (NAM, ATAF1/2, and CUC2) family transcription factor, is a key regulator of abiotic stress-induced senescence. Knocking BoNAP out via CRISPR/Cas9 genome editing in broccoli (Brassica oleracea var. italica) renders the broccoli lines more resistant to multiple abiotic stresses including drought, salt, extreme temperatures, strong UV radiation, and darkness phenotypically and physiologically. The research provides a novel strategy for breeding broccoli germplasm resilient to climate change."]},{"key":"dc:format.mimetype","label":"Dc Format Mimetype","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Breeding broccoli resilient to multi-abiotic stresses using genome editing"]}]}],"canonical_facts":{"dc:contributor.committeemember":["Griffiths, Phillip"],"dc:creator":["Meng, Wei"],"dc:date.accessioned":["2024-04-05T18:36:42Z"],"dc:date.issued":["2023-08"],"dc:description":["142 pages"],"dc:description.abstract":["Broccoli cultivars are sensitive to environmental stresses. Dramatic fluctuation in temperature and other weather conditions caused by climate change has negatively affected the growth and development of broccoli and reduced broccoli productivity and quality. Plant senescence is one of the most important consequences of all adverse environmental conditions or abiotic stresses. NAC-LIKE, ACTIVATED BY AP3/PI (NAP), a NAC (NAM, ATAF1/2, and CUC2) family transcription factor, is a key regulator of abiotic stress-induced senescence. Knocking BoNAP out via CRISPR/Cas9 genome editing in broccoli (Brassica oleracea var. italica) renders the broccoli lines more resistant to multiple abiotic stresses including drought, salt, extreme temperatures, strong UV radiation, and darkness phenotypically and physiologically. The research provides a novel strategy for breeding broccoli germplasm resilient to climate change."],"dc:format.mimetype":["application/pdf"],"dc:identifier.doi":["https://doi.org/10.7298/5c29-xh92"],"dc:identifier.other":["ProQuest Submission ID: 11728","ProQuest Publication ID: 30485200"],"dc:identifier.uri":["https://hdl.handle.net/1813/114506"],"dc:language.iso":["en"],"dc:subject":["broccoli","abiotic stress","senescence","NAP","CRISPR/Cas9","resilience"],"dc:title":["Breeding broccoli resilient to multi-abiotic stresses using genome editing"],"dc:type":["dissertation or thesis"],"thesis:degree_discipline":["Horticulture"],"thesis:degree_level":["Master of Science"],"thesis:degree_name":["M.S., Horticulture"],"thesis:institution_name":["Cornell University"]},"updated_at":"2026-07-24T01:49:02Z"}