The insulin-signaling pathway plays a crucial role in regulating metabolic homeostasis in both humans and insects. Dysregulation or mutation within this pathway can lead to various diseases, such as diabetes mellitus and insulin resistance. The insulin-like peptide 4 (ILP4) in Drosophila is an interesting subject for study due to its similarity to mammalian insulin and its role in regulating sugar metabolism. In this study, we investigate a novel splicing variation within the Drosophila takahashii Insulin-Like Peptide 4 (ILP4) gene. Our investigation identified a splicing variation found in four Drosophila species that introduces an additional codon in one isoform, resulting in an extra glutamic acid residue in the final protein structure. Initial analysis suggests that this variation may not significantly impact ILP4 functionality, but additional studies, such as protein structure modeling and function analysis, are necessary. Further identified is significant variation in sex specific ILP4 expression patterns across the Drosophila genus. Future research is called for to explore tissue-specific expression and the observed functional consequences of sequence variations. By investigating this splicing variation and sex-specific expression, this research provides a foundation for future understanding of how ILP4 functions and how the observed splicing variation might influence gene expression.
","abstract_html":"<p>The insulin-signaling pathway plays a crucial role in regulating metabolic homeostasis in both humans and insects. Dysregulation or mutation within this pathway can lead to various diseases, such as diabetes mellitus and insulin resistance. The insulin-like peptide 4 (ILP4) in Drosophila is an interesting subject for study due to its similarity to mammalian insulin and its role in regulating sugar metabolism. In this study, we investigate a novel splicing variation within the Drosophila takahashii Insulin-Like Peptide 4 (ILP4) gene. Our investigation identified a splicing variation found in four Drosophila species that introduces an additional codon in one isoform, resulting in an extra glutamic acid residue in the final protein structure. Initial analysis suggests that this variation may not significantly impact ILP4 functionality, but additional studies, such as protein structure modeling and function analysis, are necessary. Further identified is significant variation in sex specific ILP4 expression patterns across the Drosophila genus. Future research is called for to explore tissue-specific expression and the observed functional consequences of sequence variations. By investigating this splicing variation and sex-specific expression, this research provides a foundation for future understanding of how ILP4 functions and how the observed splicing variation might influence gene expression. </p>","abstract_has_math":false,"creators":["Griffith, Zachary C."],"institution":null,"degree_name":"Biology","degree_level":"Thesis","degree_discipline":"Biology","degree_department":null,"school":null,"contributors":["Dr. Brian Schwartz","Dr. Kevin Burgess","Dr. Cindy S. Ticknor"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2024,"date_issued":"2024-01-01T08:00:00Z","date_published":"2024-01-01T08:00:00Z","updated_at":"2026-07-24T01:44:41Z","subjects":["ILP4","drosophila genus","Biology"],"languages":["english"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://csuepress.columbusstate.edu/theses_dissertations/521","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Dr. Brian Schwartz","Dr. Kevin Burgess","Dr. Cindy S. Ticknor"]},{"key":"dc:creator","label":"Author","values":["Griffith, Zachary C."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2024-05-21T07:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Biology"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["ILP4","drosophila genus","Biology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["english"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://csuepress.columbusstate.edu/theses_dissertations/521"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The insulin-signaling pathway plays a crucial role in regulating metabolic homeostasis in both humans and insects. Dysregulation or mutation within this pathway can lead to various diseases, such as diabetes mellitus and insulin resistance. The insulin-like peptide 4 (ILP4) in Drosophila is an interesting subject for study due to its similarity to mammalian insulin and its role in regulating sugar metabolism. In this study, we investigate a novel splicing variation within the Drosophila takahashii Insulin-Like Peptide 4 (ILP4) gene. Our investigation identified a splicing variation found in four Drosophila species that introduces an additional codon in one isoform, resulting in an extra glutamic acid residue in the final protein structure. Initial analysis suggests that this variation may not significantly impact ILP4 functionality, but additional studies, such as protein structure modeling and function analysis, are necessary. Further identified is significant variation in sex specific ILP4 expression patterns across the Drosophila genus. Future research is called for to explore tissue-specific expression and the observed functional consequences of sequence variations. By investigating this splicing variation and sex-specific expression, this research provides a foundation for future understanding of how ILP4 functions and how the observed splicing variation might influence gene expression.
"]},{"key":"dc:title","label":"Title","values":["An Investigation Into Splicing Variation and Expression Patterns of Insulin-Like Peptide 4 (ILP4) Within the Drosophila Genus"]}]}],"canonical_facts":{"dc:contributor":["Dr. Brian Schwartz","Dr. Kevin Burgess","Dr. Cindy S. Ticknor"],"dc:creator":["Griffith, Zachary C."],"dc:date.available":["2024-05-21T07:00:00Z"],"dc:description.abstract":["The insulin-signaling pathway plays a crucial role in regulating metabolic homeostasis in both humans and insects. Dysregulation or mutation within this pathway can lead to various diseases, such as diabetes mellitus and insulin resistance. The insulin-like peptide 4 (ILP4) in Drosophila is an interesting subject for study due to its similarity to mammalian insulin and its role in regulating sugar metabolism. In this study, we investigate a novel splicing variation within the Drosophila takahashii Insulin-Like Peptide 4 (ILP4) gene. Our investigation identified a splicing variation found in four Drosophila species that introduces an additional codon in one isoform, resulting in an extra glutamic acid residue in the final protein structure. Initial analysis suggests that this variation may not significantly impact ILP4 functionality, but additional studies, such as protein structure modeling and function analysis, are necessary. Further identified is significant variation in sex specific ILP4 expression patterns across the Drosophila genus. Future research is called for to explore tissue-specific expression and the observed functional consequences of sequence variations. By investigating this splicing variation and sex-specific expression, this research provides a foundation for future understanding of how ILP4 functions and how the observed splicing variation might influence gene expression.
"],"dc:identifier":["https://csuepress.columbusstate.edu/theses_dissertations/521"],"dc:language":["english"],"dc:subject":["ILP4","drosophila genus","Biology"],"dc:title":["An Investigation Into Splicing Variation and Expression Patterns of Insulin-Like Peptide 4 (ILP4) Within the Drosophila Genus"],"thesis:degree_discipline":["Biology"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["Biology"]},"updated_at":"2026-07-24T01:44:41Z"}