We studied the effects of ethynodiol diacetate on the viability of hydrogen peroxide stressed glial cells. We wanted to determine the extent in which varying concentration of ethynodiol diacetate affect cell viability of cultured glial cells from hydrogen peroxide toxicity. Based on previous studies, we predicted that the viability of hydrogen peroxide-stressed cells will increase with the presence of the progesterone, ethynodiol diacetate, and that cell viability will increase with increasing concentration of ethynodiol diacetate. Glial cells of 1x10° cells/well were introduced into a 96-well plate and allowed to grow for 24 hours in an incubator at 37°C and 5% CO2. Cells were treated with 0 nM (control), 10 nM, 100 nM, 1 uM, 10 uM ethynodiol diacetate in doublets, with half of the cells being stressed with hydrogen peroxide. Data were analyzed using a 2-way ANOVA and a Tukey’s post hoc test with a p-value of less than 0.05 being significant. Viability did not differ significantly between stressed and unstressed cells (2-way ANOVA, F9,30=1.673, P=0.211) or between cells receiving varying concentrations of ethynodiol diacetate (2-way ANOVA, F9,30=0.638 P=0.641). The results illustrate that ethynodiol diacetate, when under these treatment parameters, does not have protective effects on the glial cell cultures.
","abstract_html":"<p>We studied the effects of ethynodiol diacetate on the viability of hydrogen peroxide stressed glial cells. We wanted to determine the extent in which varying concentration of ethynodiol diacetate affect cell viability of cultured glial cells from hydrogen peroxide toxicity. Based on previous studies, we predicted that the viability of hydrogen peroxide-stressed cells will increase with the presence of the progesterone, ethynodiol diacetate, and that cell viability will increase with increasing concentration of ethynodiol diacetate. Glial cells of 1x10° cells/well were introduced into a 96-well plate and allowed to grow for 24 hours in an incubator at 37°C and 5% CO2. Cells were treated with 0 nM (control), 10 nM, 100 nM, 1 uM, 10 uM ethynodiol diacetate in doublets, with half of the cells being stressed with hydrogen peroxide. Data were analyzed using a 2-way ANOVA and a Tukey’s post hoc test with a p-value of less than 0.05 being significant. Viability did not differ significantly between stressed and unstressed cells (2-way ANOVA, F9,30=1.673, P=0.211) or between cells receiving varying concentrations of ethynodiol diacetate (2-way ANOVA, F9,30=0.638 P=0.641). The results illustrate that ethynodiol diacetate, when under these treatment parameters, does not have protective effects on the glial cell cultures.</p>","abstract_has_math":false,"creators":["Guthrie, Tracee L"],"institution":null,"degree_name":"Biology","degree_level":"Thesis","degree_discipline":"Biology","degree_department":null,"school":null,"contributors":["Dr. Kathleen Hughes","Dr. Lauren King"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2019,"date_issued":"2019-01-01T08:00:00Z","date_published":"2019-01-01T08:00:00Z","updated_at":"2026-07-24T01:45:01Z","subjects":["Ethynodiol Diacetate","Hydrogen Peroxide","Stressed Glial Cells","Hydrogen Peroxide Toxicity","Biochemistry","Biology"],"languages":["English"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://csuepress.columbusstate.edu/theses_dissertations/425","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Dr. Kathleen Hughes","Dr. Lauren King"]},{"key":"dc:creator","label":"Author","values":["Guthrie, Tracee L"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2021-05-04T07:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Biology"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Ethynodiol Diacetate","Hydrogen Peroxide","Stressed Glial Cells","Hydrogen Peroxide Toxicity","Biochemistry","Biology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["English"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://csuepress.columbusstate.edu/theses_dissertations/425"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["We studied the effects of ethynodiol diacetate on the viability of hydrogen peroxide stressed glial cells. We wanted to determine the extent in which varying concentration of ethynodiol diacetate affect cell viability of cultured glial cells from hydrogen peroxide toxicity. Based on previous studies, we predicted that the viability of hydrogen peroxide-stressed cells will increase with the presence of the progesterone, ethynodiol diacetate, and that cell viability will increase with increasing concentration of ethynodiol diacetate. Glial cells of 1x10° cells/well were introduced into a 96-well plate and allowed to grow for 24 hours in an incubator at 37°C and 5% CO2. Cells were treated with 0 nM (control), 10 nM, 100 nM, 1 uM, 10 uM ethynodiol diacetate in doublets, with half of the cells being stressed with hydrogen peroxide. Data were analyzed using a 2-way ANOVA and a Tukey’s post hoc test with a p-value of less than 0.05 being significant. Viability did not differ significantly between stressed and unstressed cells (2-way ANOVA, F9,30=1.673, P=0.211) or between cells receiving varying concentrations of ethynodiol diacetate (2-way ANOVA, F9,30=0.638 P=0.641). The results illustrate that ethynodiol diacetate, when under these treatment parameters, does not have protective effects on the glial cell cultures.
"]},{"key":"dc:title","label":"Title","values":["The Involvement of Progesterone in Glial Cell Viability"]}]}],"canonical_facts":{"dc:contributor":["Dr. Kathleen Hughes","Dr. Lauren King"],"dc:creator":["Guthrie, Tracee L"],"dc:date.available":["2021-05-04T07:00:00Z"],"dc:description.abstract":["We studied the effects of ethynodiol diacetate on the viability of hydrogen peroxide stressed glial cells. We wanted to determine the extent in which varying concentration of ethynodiol diacetate affect cell viability of cultured glial cells from hydrogen peroxide toxicity. Based on previous studies, we predicted that the viability of hydrogen peroxide-stressed cells will increase with the presence of the progesterone, ethynodiol diacetate, and that cell viability will increase with increasing concentration of ethynodiol diacetate. Glial cells of 1x10° cells/well were introduced into a 96-well plate and allowed to grow for 24 hours in an incubator at 37°C and 5% CO2. Cells were treated with 0 nM (control), 10 nM, 100 nM, 1 uM, 10 uM ethynodiol diacetate in doublets, with half of the cells being stressed with hydrogen peroxide. Data were analyzed using a 2-way ANOVA and a Tukey’s post hoc test with a p-value of less than 0.05 being significant. Viability did not differ significantly between stressed and unstressed cells (2-way ANOVA, F9,30=1.673, P=0.211) or between cells receiving varying concentrations of ethynodiol diacetate (2-way ANOVA, F9,30=0.638 P=0.641). The results illustrate that ethynodiol diacetate, when under these treatment parameters, does not have protective effects on the glial cell cultures.
"],"dc:identifier":["https://csuepress.columbusstate.edu/theses_dissertations/425"],"dc:language":["English"],"dc:subject":["Ethynodiol Diacetate","Hydrogen Peroxide","Stressed Glial Cells","Hydrogen Peroxide Toxicity","Biochemistry","Biology"],"dc:title":["The Involvement of Progesterone in Glial Cell Viability"],"thesis:degree_discipline":["Biology"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["Biology"]},"updated_at":"2026-07-24T01:45:01Z"}