{"id":{"repo_id":"columbia-diss","oai_identifier":"oai:academiccommons.columbia.edu:10.7916/D8HD82N6"},"canonical_url":"https://search.dev.ndltd.org/etd/columbia-diss/oai:academiccommons.columbia.edu:10.7916/D8HD82N6","repository":{"repo_id":"columbia-diss","name":"Columbia University","base_url":"https://academiccommons.columbia.edu/oai"},"display":{"title":"Functional Imaging Through Dark State Dynamics","abstract":"This thesis harnesses the environmental sensitivity of the dark states of molecular fluorophores, both endogeneous in cells/tissue and externally introduced for mapping of chemical micro-environments including factors such as ion concentration and microviscosity. A novel technique for directly detecting the dynamics and population of dark states, such as the lowest triplet state, was developed and called FAPA, Fluorescence Anomalous Phase Advance. This technique is a sensitive and fast reporter of dark state dynamics and can be used for imaging. In addition, a genetically-encoded microviscosity and micro-environmental mapping ability with high protein-specific specificity was developed by using a TMP-tag and Fluorescence Lifetime Imaging.","abstract_html":"This thesis harnesses the environmental sensitivity of the dark states of molecular fluorophores, both endogeneous in cells/tissue and externally introduced for mapping of chemical micro-environments including factors such as ion concentration and microviscosity. A novel technique for directly detecting the dynamics and population of dark states, such as the lowest triplet state, was developed and called FAPA, Fluorescence Anomalous Phase Advance. This technique is a sensitive and fast reporter of dark state dynamics and can be used for imaging. In addition, a genetically-encoded microviscosity and micro-environmental mapping ability with high protein-specific specificity was developed by using a TMP-tag and Fluorescence Lifetime Imaging.","abstract_has_math":false,"creators":["Gatzogiannis, Evangelos George"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011","date_published":"2011","updated_at":"2026-07-24T01:44:16Z","subjects":["Chemistry, Physical and theoretical","Fluorescent probes","Fluorescence","Triplet state"],"languages":["English"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://doi.org/10.7916/D8HD82N6","outbound_label":"DOI","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Gatzogiannis, Evangelos George"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011"]},{"key":"dc:type","label":"Dc Type","values":["Theses"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Chemistry, Physical and theoretical","Fluorescent probes","Fluorescence","Triplet state"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["English"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://doi.org/10.7916/D8HD82N6"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["This thesis harnesses the environmental sensitivity of the dark states of molecular fluorophores, both endogeneous in cells/tissue and externally introduced for mapping of chemical micro-environments including factors such as ion concentration and microviscosity. A novel technique for directly detecting the dynamics and population of dark states, such as the lowest triplet state, was developed and called FAPA, Fluorescence Anomalous Phase Advance. This technique is a sensitive and fast reporter of dark state dynamics and can be used for imaging. In addition, a genetically-encoded microviscosity and micro-environmental mapping ability with high protein-specific specificity was developed by using a TMP-tag and Fluorescence Lifetime Imaging."]},{"key":"dc:title","label":"Title","values":["Functional Imaging Through Dark State Dynamics"]}]}],"canonical_facts":{"dc:creator":["Gatzogiannis, Evangelos George"],"dc:date":["2011"],"dc:description":["This thesis harnesses the environmental sensitivity of the dark states of molecular fluorophores, both endogeneous in cells/tissue and externally introduced for mapping of chemical micro-environments including factors such as ion concentration and microviscosity. A novel technique for directly detecting the dynamics and population of dark states, such as the lowest triplet state, was developed and called FAPA, Fluorescence Anomalous Phase Advance. This technique is a sensitive and fast reporter of dark state dynamics and can be used for imaging. In addition, a genetically-encoded microviscosity and micro-environmental mapping ability with high protein-specific specificity was developed by using a TMP-tag and Fluorescence Lifetime Imaging."],"dc:identifier":["https://doi.org/10.7916/D8HD82N6"],"dc:language":["English"],"dc:subject":["Chemistry, Physical and theoretical","Fluorescent probes","Fluorescence","Triplet state"],"dc:title":["Functional Imaging Through Dark State Dynamics"],"dc:type":["Theses"]},"updated_at":"2026-07-24T01:44:16Z"}