{"id":{"repo_id":"colostate","oai_identifier":"oai:mountainscholar.org:10217/81020"},"canonical_url":"https://search.dev.ndltd.org/etd/colostate/oai:mountainscholar.org:10217/81020","repository":{"repo_id":"colostate","name":"Colorado State University","base_url":"https://api.mountainscholar.org/server/oai/request"},"display":{"title":"Correlation of HAS2-associated gene duplications with biological aggressiveness of mast cell tumors in Chinese Shar-Pei dogs","abstract":"Cutaneous mucinosis in Shar-Pei dogs is the result of excessive dermal hyaluronan, a protein associated with angiogenesis and tumor cell motility in multiple human and canine neoplasms. Cutaneous mucinosis in Shar-Pei has been associated with gene duplications upstream of the hyaluronic acid synthase 2 gene (HAS2). The objective of this study was to evaluate the relationship between HAS2, cutaneous mucinosis, and features of mast cell tumor (MCT) aggressiveness in Shar-Pei dogs. Biopsies of cutaneous MCTs from 149 Shar-Pei and 100 non-Shar-Pei were graded according to two schemes for canine cutaneous MCTs. Biopsies of the Shar-Pei MCTs were also evaluated for degree of cutaneous mucinosis, depth of invasion, and microvessel density (MVD). Shar-Pei and non-Shar-Pei MCTs were evaluated via qPCR for relative copy number of the gene duplication upstream of HAS2. The proportion of grade III tumors was significantly higher in Shar-Pei than the general canine population (p=1.044e-11), with no difference in average age at diagnosis. Shar-Pei biopsies had significantly higher HAS2-associated gene segment duplications than non-Shar-Pei (p=1.128e-11), and copy number was significantly associated with the development of grade III tumors (p=0.0077), mitotic index > or = 7 (p=0.022), and tumoral MVD (p<0.05). Relative copy number was not significantly associated with the degree of cutaneous mucinosis or depth of invasion. Our data suggest a relationship between HAS2 gene duplications and features of MCT aggressiveness in Shar-Pei dogs.","abstract_html":"Cutaneous mucinosis in Shar-Pei dogs is the result of excessive dermal hyaluronan, a protein associated with angiogenesis and tumor cell motility in multiple human and canine neoplasms. Cutaneous mucinosis in Shar-Pei has been associated with gene duplications upstream of the hyaluronic acid synthase 2 gene (HAS2). The objective of this study was to evaluate the relationship between HAS2, cutaneous mucinosis, and features of mast cell tumor (MCT) aggressiveness in Shar-Pei dogs. Biopsies of cutaneous MCTs from 149 Shar-Pei and 100 non-Shar-Pei were graded according to two schemes for canine cutaneous MCTs. Biopsies of the Shar-Pei MCTs were also evaluated for degree of cutaneous mucinosis, depth of invasion, and microvessel density (MVD). Shar-Pei and non-Shar-Pei MCTs were evaluated via qPCR for relative copy number of the gene duplication upstream of HAS2. The proportion of grade III tumors was significantly higher in Shar-Pei than the general canine population (p=1.044e-11), with no difference in average age at diagnosis. Shar-Pei biopsies had significantly higher HAS2-associated gene segment duplications than non-Shar-Pei (p=1.128e-11), and copy number was significantly associated with the development of grade III tumors (p=0.0077), mitotic index &gt; or = 7 (p=0.022), and tumoral MVD (p&lt;0.05). Relative copy number was not significantly associated with the degree of cutaneous mucinosis or depth of invasion. Our data suggest a relationship between HAS2 gene duplications and features of MCT aggressiveness in Shar-Pei dogs.","abstract_has_math":false,"creators":["Garner, Alana Pavuk, author","Avery, Anne, advisor","Thamm, Douglas, committee member","Basaraba, Randall, committee member"],"institution":"Colorado State University. Libraries","degree_name":"Master of Science (M.S.)","degree_level":"Masters","degree_discipline":"Microbiology, Immunology, and Pathology","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2013,"date_issued":"2013","date_published":"2013","updated_at":"2026-07-27T19:12:52Z","subjects":["mast cell tumor","Shar-Pei dog"],"languages":["eng","English"],"rights":["Copyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright."],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://doi.org/10.25675/3.018243"],"render_values":[{"text":"https://doi.org/10.25675/3.018243","href":"https://doi.org/10.25675/3.018243","code":true}]},{"key":"dc:identifier","label":"Identifier","values":["ETDF2013500374MIPA"],"render_values":[{"text":"ETDF2013500374MIPA","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/10217/81020","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Garner, Alana Pavuk, author","Avery, Anne, advisor","Thamm, Douglas, committee member","Basaraba, Randall, committee member"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2007-01-03T06:11:25Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2007-01-03T06:11:25Z"]},{"key":"dc:date.issued","label":"Date","values":["2013"]},{"key":"dc:publisher","label":"Institution","values":["Colorado State University. 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The objective of this study was to evaluate the relationship between HAS2, cutaneous mucinosis, and features of mast cell tumor (MCT) aggressiveness in Shar-Pei dogs. Biopsies of cutaneous MCTs from 149 Shar-Pei and 100 non-Shar-Pei were graded according to two schemes for canine cutaneous MCTs. Biopsies of the Shar-Pei MCTs were also evaluated for degree of cutaneous mucinosis, depth of invasion, and microvessel density (MVD). Shar-Pei and non-Shar-Pei MCTs were evaluated via qPCR for relative copy number of the gene duplication upstream of HAS2. The proportion of grade III tumors was significantly higher in Shar-Pei than the general canine population (p=1.044e-11), with no difference in average age at diagnosis. Shar-Pei biopsies had significantly higher HAS2-associated gene segment duplications than non-Shar-Pei (p=1.128e-11), and copy number was significantly associated with the development of grade III tumors (p=0.0077), mitotic index > or = 7 (p=0.022), and tumoral MVD (p<0.05). Relative copy number was not significantly associated with the degree of cutaneous mucinosis or depth of invasion. Our data suggest a relationship between HAS2 gene duplications and features of MCT aggressiveness in Shar-Pei dogs."]},{"key":"dc:format.medium","label":"Dc Format Medium","values":["born digital","masters theses"]},{"key":"dc:title","label":"Title","values":["Correlation of HAS2-associated gene duplications with biological aggressiveness of mast cell tumors in Chinese Shar-Pei dogs"]}]}],"canonical_facts":{"dc:creator":["Garner, Alana Pavuk, author","Avery, Anne, advisor","Thamm, Douglas, committee member","Basaraba, Randall, committee member"],"dc:date.accessioned":["2007-01-03T06:11:25Z"],"dc:date.available":["2007-01-03T06:11:25Z"],"dc:date.issued":["2013"],"dc:description.abstract":["Cutaneous mucinosis in Shar-Pei dogs is the result of excessive dermal hyaluronan, a protein associated with angiogenesis and tumor cell motility in multiple human and canine neoplasms. Cutaneous mucinosis in Shar-Pei has been associated with gene duplications upstream of the hyaluronic acid synthase 2 gene (HAS2). The objective of this study was to evaluate the relationship between HAS2, cutaneous mucinosis, and features of mast cell tumor (MCT) aggressiveness in Shar-Pei dogs. Biopsies of cutaneous MCTs from 149 Shar-Pei and 100 non-Shar-Pei were graded according to two schemes for canine cutaneous MCTs. Biopsies of the Shar-Pei MCTs were also evaluated for degree of cutaneous mucinosis, depth of invasion, and microvessel density (MVD). Shar-Pei and non-Shar-Pei MCTs were evaluated via qPCR for relative copy number of the gene duplication upstream of HAS2. The proportion of grade III tumors was significantly higher in Shar-Pei than the general canine population (p=1.044e-11), with no difference in average age at diagnosis. Shar-Pei biopsies had significantly higher HAS2-associated gene segment duplications than non-Shar-Pei (p=1.128e-11), and copy number was significantly associated with the development of grade III tumors (p=0.0077), mitotic index > or = 7 (p=0.022), and tumoral MVD (p<0.05). Relative copy number was not significantly associated with the degree of cutaneous mucinosis or depth of invasion. Our data suggest a relationship between HAS2 gene duplications and features of MCT aggressiveness in Shar-Pei dogs."],"dc:format.medium":["born digital","masters theses"],"dc:identifier":["Garner_colostate_0053N_12109.pdf","ETDF2013500374MIPA"],"dc:identifier.uri":["http://hdl.handle.net/10217/81020","https://doi.org/10.25675/3.018243"],"dc:language":["English"],"dc:language.iso":["eng"],"dc:publisher":["Colorado State University. Libraries"],"dc:rights":["Copyright and other restrictions may apply. User is responsible for compliance with all applicable laws. 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