{"id":{"repo_id":"colostate","oai_identifier":"oai:mountainscholar.org:10217/44849"},"canonical_url":"https://search.dev.ndltd.org/etd/colostate/oai:mountainscholar.org:10217/44849","repository":{"repo_id":"colostate","name":"Colorado State University","base_url":"https://api.mountainscholar.org/server/oai/request"},"display":{"title":"T cell mediated satellite cell function: implications for age-associated changes in skeletal muscle regeneration","abstract":"Sarcopenia is an age-associated loss of skeletal muscle mass and strength. Recent evidence suggests that an age-associated loss of muscle precursor cell (MPC) functionality contributes to sarcopenia. Current research also suggests that T cells of the immune system may influence skeletal muscle repair via signaling with MPCs. The objective of the present study was to examine the influence of activated T cells on MPCs. MPCs were collected from the gastrocnemius and plantaris from 3-mo-old (young) and 32-mo-old (old) animals. Splenic T cells were also harvested using anti-CD3 Dynabead isolation. T cells were activated for 48 hours with co-stimulation of 100 IU/ml Interleukin-2 (IL-2) and 5 ug/ml of anti-CD28. Co-stimulation increased 5-bromo-2'-deoxyuridine (BrdU) incorporation (proliferation) of T cells from 13.382% (SEM=4.55, n=5) in control to 64.77% (SEM= 6.02, n=5). Additionally, T cell cytokines increased MPC proliferation by 23.98% (SEM=5.69, n=4) in young MPCs but decreased by 1.58% (SEM=4.09, n=4) in old MPCs. T cell cytokines were also found to be chemoattractant. Young MPCs migrated at a rate of 1.36 (SEM=0.56, n=4) with T cell cytokines. Old MPCs, however, did not migrate with T cell cytokines -0.05 (SEM= 0.214, n=4). These data suggest that T cells may play a critical role in mediating MPC function. Furthermore, aging may alter T cell-induced MPC function. These findings have implications for developing strategies aimed at increasing MPC proliferation and the regenerative capacity of aged skeletal muscle.","abstract_html":"Sarcopenia is an age-associated loss of skeletal muscle mass and strength. Recent evidence suggests that an age-associated loss of muscle precursor cell (MPC) functionality contributes to sarcopenia. Current research also suggests that T cells of the immune system may influence skeletal muscle repair via signaling with MPCs. The objective of the present study was to examine the influence of activated T cells on MPCs. MPCs were collected from the gastrocnemius and plantaris from 3-mo-old (young) and 32-mo-old (old) animals. Splenic T cells were also harvested using anti-CD3 Dynabead isolation. T cells were activated for 48 hours with co-stimulation of 100 IU/ml Interleukin-2 (IL-2) and 5 ug/ml of anti-CD28. Co-stimulation increased 5-bromo-2'-deoxyuridine (BrdU) incorporation (proliferation) of T cells from 13.382% (SEM=4.55, n=5) in control to 64.77% (SEM= 6.02, n=5). Additionally, T cell cytokines increased MPC proliferation by 23.98% (SEM=5.69, n=4) in young MPCs but decreased by 1.58% (SEM=4.09, n=4) in old MPCs. T cell cytokines were also found to be chemoattractant. Young MPCs migrated at a rate of 1.36 (SEM=0.56, n=4) with T cell cytokines. Old MPCs, however, did not migrate with T cell cytokines -0.05 (SEM= 0.214, n=4). These data suggest that T cells may play a critical role in mediating MPC function. Furthermore, aging may alter T cell-induced MPC function. These findings have implications for developing strategies aimed at increasing MPC proliferation and the regenerative capacity of aged skeletal muscle.","abstract_has_math":false,"creators":["Dumke, Breanna R., author","Lees, Simon J., advisor","Gotshall, Robert W., advisor","Frye, Melinda A., committee member"],"institution":"Colorado State University. Libraries","degree_name":"Master of Science (M.S.)","degree_level":"Masters","degree_discipline":"Health and Exercise Science","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2010,"date_issued":"2010","date_published":"2010","updated_at":"2026-07-27T19:12:52Z","subjects":["T cell","skeletal muscle","satellite cell","proliferation","migration","conditioned media","Muscles -- Diseases","Muscles -- Regeneration","T cells -- Therapeutic use"],"languages":["eng","English"],"rights":["Copyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright."],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://doi.org/10.25675/3.018278"],"render_values":[{"text":"https://doi.org/10.25675/3.018278","href":"https://doi.org/10.25675/3.018278","code":true}]},{"key":"dc:identifier","label":"Identifier","values":["ETDF2010300010HAES"],"render_values":[{"text":"ETDF2010300010HAES","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/10217/44849","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Dumke, Breanna R., author","Lees, Simon J., advisor","Gotshall, Robert W., advisor","Frye, Melinda A., committee member"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2007-01-03T04:51:31Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2007-01-03T04:51:31Z"]},{"key":"dc:date.issued","label":"Date","values":["2010"]},{"key":"dc:publisher","label":"Institution","values":["Colorado State University. Libraries"]},{"key":"dc:type","label":"Dc Type","values":["Text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Health and Exercise Science"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science (M.S.)"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["Colorado State University"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["T cell","skeletal muscle","satellite cell","proliferation","migration","conditioned media","Muscles -- Diseases","Muscles -- Regeneration","T cells -- Therapeutic use"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["English"]},{"key":"dc:language.iso","label":"Language (ISO)","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["2010_Fall_Dumke_Brianna.pdf","ETDF2010300010HAES"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/10217/44849","https://doi.org/10.25675/3.018278"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Sarcopenia is an age-associated loss of skeletal muscle mass and strength. Recent evidence suggests that an age-associated loss of muscle precursor cell (MPC) functionality contributes to sarcopenia. Current research also suggests that T cells of the immune system may influence skeletal muscle repair via signaling with MPCs. The objective of the present study was to examine the influence of activated T cells on MPCs. MPCs were collected from the gastrocnemius and plantaris from 3-mo-old (young) and 32-mo-old (old) animals. Splenic T cells were also harvested using anti-CD3 Dynabead isolation. T cells were activated for 48 hours with co-stimulation of 100 IU/ml Interleukin-2 (IL-2) and 5 ug/ml of anti-CD28. Co-stimulation increased 5-bromo-2'-deoxyuridine (BrdU) incorporation (proliferation) of T cells from 13.382% (SEM=4.55, n=5) in control to 64.77% (SEM= 6.02, n=5). Additionally, T cell cytokines increased MPC proliferation by 23.98% (SEM=5.69, n=4) in young MPCs but decreased by 1.58% (SEM=4.09, n=4) in old MPCs. T cell cytokines were also found to be chemoattractant. Young MPCs migrated at a rate of 1.36 (SEM=0.56, n=4) with T cell cytokines. Old MPCs, however, did not migrate with T cell cytokines -0.05 (SEM= 0.214, n=4). These data suggest that T cells may play a critical role in mediating MPC function. Furthermore, aging may alter T cell-induced MPC function. These findings have implications for developing strategies aimed at increasing MPC proliferation and the regenerative capacity of aged skeletal muscle."]},{"key":"dc:format.medium","label":"Dc Format Medium","values":["masters theses"]},{"key":"dc:title","label":"Title","values":["T cell mediated satellite cell function: implications for age-associated changes in skeletal muscle regeneration"]}]}],"canonical_facts":{"dc:creator":["Dumke, Breanna R., author","Lees, Simon J., advisor","Gotshall, Robert W., advisor","Frye, Melinda A., committee member"],"dc:date.accessioned":["2007-01-03T04:51:31Z"],"dc:date.available":["2007-01-03T04:51:31Z"],"dc:date.issued":["2010"],"dc:description.abstract":["Sarcopenia is an age-associated loss of skeletal muscle mass and strength. Recent evidence suggests that an age-associated loss of muscle precursor cell (MPC) functionality contributes to sarcopenia. Current research also suggests that T cells of the immune system may influence skeletal muscle repair via signaling with MPCs. The objective of the present study was to examine the influence of activated T cells on MPCs. MPCs were collected from the gastrocnemius and plantaris from 3-mo-old (young) and 32-mo-old (old) animals. Splenic T cells were also harvested using anti-CD3 Dynabead isolation. T cells were activated for 48 hours with co-stimulation of 100 IU/ml Interleukin-2 (IL-2) and 5 ug/ml of anti-CD28. Co-stimulation increased 5-bromo-2'-deoxyuridine (BrdU) incorporation (proliferation) of T cells from 13.382% (SEM=4.55, n=5) in control to 64.77% (SEM= 6.02, n=5). Additionally, T cell cytokines increased MPC proliferation by 23.98% (SEM=5.69, n=4) in young MPCs but decreased by 1.58% (SEM=4.09, n=4) in old MPCs. T cell cytokines were also found to be chemoattractant. Young MPCs migrated at a rate of 1.36 (SEM=0.56, n=4) with T cell cytokines. Old MPCs, however, did not migrate with T cell cytokines -0.05 (SEM= 0.214, n=4). These data suggest that T cells may play a critical role in mediating MPC function. Furthermore, aging may alter T cell-induced MPC function. These findings have implications for developing strategies aimed at increasing MPC proliferation and the regenerative capacity of aged skeletal muscle."],"dc:format.medium":["masters theses"],"dc:identifier":["2010_Fall_Dumke_Brianna.pdf","ETDF2010300010HAES"],"dc:identifier.uri":["http://hdl.handle.net/10217/44849","https://doi.org/10.25675/3.018278"],"dc:language":["English"],"dc:language.iso":["eng"],"dc:publisher":["Colorado State University. Libraries"],"dc:rights":["Copyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright."],"dc:subject":["T cell","skeletal muscle","satellite cell","proliferation","migration","conditioned media","Muscles -- Diseases","Muscles -- Regeneration","T cells -- Therapeutic use"],"dc:title":["T cell mediated satellite cell function: implications for age-associated changes in skeletal muscle regeneration"],"dc:type":["Text"],"thesis:degree_discipline":["Health and Exercise Science"],"thesis:degree_level":["Masters"],"thesis:degree_name":["Master of Science (M.S.)"],"thesis:institution_name":["Colorado State University"]},"updated_at":"2026-07-27T19:12:52Z"}