Control of protein adsorption, cell morphology and intracellular signal transduction using self-assembled monolayers of alkylthiolates on gold
type: CreativeWork
description: Self-assembled monolayers of terminally-functionaiized alkylthiolates on gold have been used to interrogate cell-biomaterial surface interactions at the extracellular and intracellular level. The goal of this research was to provide molecular level information on the surface determinants necessary to produce predictable, controllable biological responses to implanted materials. Various players in "outside-in" communication between a cell and a material's surface were investigated. Surfaces of a variety of alkylthiol chemistries were used to monitor protein adsorption and subsequent Swiss 3T3 fibroblast adhesion, spreading and growth. Distinct alkylthiol substrate chemistry-dependent differences were observed. Formation of focal contacts and stress fibers, early indicators of effective signaling, was observed to be surface-chemistry dependent. These responses could be correlated with protein dynamics on different surface chemistries. Relative deposition and conformations of fibronectin were shown to be dependent on surface chemistry. Results indicate that observed differential cell adhesion, spreading and growth on derivatized surfaces is mediated through modulations of adsorbed ECM molecules (e.g., fibronectin). The primary regulators of the events investigated above were examined. Well-controlled alkylthiol surface chemistries were used to monitor the activation state of RhoA. Activation states of RhoA were determined and shown to be surface chemistry-dependent RhoGDI levels and intracellular localization were also shown to be surface-chemistry dependent Cells cultured on -CH3 terminated SAMs, which normally exhibit a cultured on -CH3 terminated SAMs, which normally exhibit a low growth phenotype, were transfected with a constitutively active RhoA mutant. Transfected cells exhibited significant increases in cel! length. However, no focal contact formation was observed. These results show that genetic alteration of intracellular regulators is incapable of overcoming…
abstract: Self-assembled monolayers of terminally-functionaiized alkylthiolates on gold have been used to interrogate cell-biomaterial surface interactions at the extracellular and intracellular level. The goal of this research was to provide molecular level information on the surface determinants necessary to produce predictable, controllable biological responses to implanted materials. Various players in "outside-in" communication between a cell and a material's surface were investigated. Surfaces of a variety of alkylthiol chemistries were used to monitor protein adsorption and subsequent Swiss 3T3 fibroblast adhesion, spreading and growth. Distinct alkylthiol substrate chemistry-dependent differences were observed. Formation of focal contacts and stress fibers, early indicators of effective signaling, was observed to be surface-chemistry dependent. These responses could be correlated with protein dynamics on different surface chemistries. Relative deposition and conformations of fibronectin were shown to be dependent on surface chemistry. Results indicate that observed differential cell adhesion, spreading and growth on derivatized surfaces is mediated through modulations of adsorbed ECM molecules (e.g., fibronectin). The primary regulators of the events investigated above were examined. Well-controlled alkylthiol surface chemistries were used to monitor the activation state of RhoA. Activation states of RhoA were determined and shown to be surface chemistry-dependent RhoGDI levels and intracellular localization were also shown to be surface-chemistry dependent Cells cultured on -CH3 terminated SAMs, which normally exhibit a cultured on -CH3 terminated SAMs, which normally exhibit a low growth phenotype, were transfected with a constitutively active RhoA mutant. Transfected cells exhibited significant increases in cel! length. However, no focal contact formation was observed. These results show that genetic alteration of intracellular regulators is incapable of overcoming…
author: McClary, Kristin Beth, author
author: Curthoys, Norman, advisor
author: Woody, Robert W., committee member
author: Bamburg, James R., committee member
author: Grainger, David, committee member
language: eng
language: English
keywords: cellular biology
date published: 1999
date modified: 2026-07-27T19:13:11Z
provider: Colorado State University
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Colorado State University. Libraries

PropertyValue
property ID: Handle
value: https://hdl.handle.net/10217/243954

PropertyValue
property ID: DOI
value: 10.25675/3.026620