Energy substrates, metabolic regulators, and lipid accumulation during culture of in vitro-produced bovine embryos

The main objective of this experiment was to optimize in vitro culture conditions for bovine embryonic development, using alternative energy sources and metabolic regulators. Replacing glucose with fructose in culture medium consistently increased blastocyst production per oocyte and decreased lipid content in bovine embryos. The use of phenazine ethosulphate (PES) or fetal calf serum (FCS) supplementation did not affect embryonic development; however, PES consistently decreased, and FCS increased lipid content of embryos compared to the control. There was no effect of glucose or fructose on survival of embryos after cryopreservation by slow freezing or vitrification; however, embryos-treated with PES to reduce lipid content resulted in improved cryotolerance, and FCS decreased cryotolerance compared to the control. Transfer of embryos treated with PES during in vitro culture did not affect pregnancy rates, conceptus losses, or fetal or post-natal development in calves born normally. The sex ratio of calves born was skewed toward males. This effect likely was due to a toxic effect of glucose to female embryos cultured in vitro. Therefore, the more expanded day 7 blastocysts were mostly male embryos.

Name thesis:degree_name

Doctor of Philosophy (Ph.D.)

Level thesis:degree_level

Doctoral

Discipline thesis:degree_discipline

Biomedical Sciences

Grantor dc:publisher

Colorado State University. Libraries

Year dc:date.issued

2007

Authors dc:creator

• Barceló-Fimbres, Moisés, author
• Seidel, George E., Jr., advisor

• bovine embryos
• energy substrates
• fructose
• glucose
• lipid
• metabolic
• anatomy and physiology
• animals
• environmental engineering

Statement dc:rights

• Copyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.

Language dc:language.iso

eng, English

Identifier URI

https://doi.org/10.25675/3.018266

OAI identifier oai:identifier

oai:mountainscholar.org:10217/237572