Roles of piwi-interacting RNAs and small interfering RNAs in regulating gene expression in Caenorhabditis elegans

There are three distinct classes of small RNAs found in animals: microRNAs (miRNAs), piwi interacting RNAs (piRNAs), and small interfering RNAs (siRNAs). miRNAs are required throughout development, whereas piRNAs and siRNAs are best known for their roles in germline development and genome defense (see Chapter 1 for an overview of piRNA and siRNA pathways). To identify the roles of piRNAs and siRNAs in regulating gene expression in the germ cells of Caenorhabditis elegans, we subjected small RNAs and messenger RNAs (mRNAs) from the distal gonads of piRNA and siRNA mutants to high-throughput sequencing. We identified extensive roles for piRNAs and siRNAs in regulating gene expression during germline development, including an unexpected role for piRNAs in preventing histone silencing by the siRNA pathway (see Chapter 2).

Name thesis:degree_name

Master of Science (M.S.)

Level thesis:degree_level

Masters

Discipline thesis:degree_discipline

Cell and Molecular Biology

Grantor dc:publisher

Colorado State University. Libraries

Year dc:date.issued

2022

Authors dc:creator

• Reed, Kailee Janelle, author
• Montgomery, Taiowa A., advisor
• Wilusz, Carol J., committee member
• Nishimura, Marc T., committee member
• Hoerndli, Fred J., committee member

Statement dc:rights

• Copyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.

Language dc:language.iso

eng, English

Identifier URI

https://doi.org/10.25675/3.02323

OAI identifier oai:identifier

oai:mountainscholar.org:10217/235162