Localization of nerve growth factor-β in the stallion reproductive tract

Nerve growth factor-β (NGF-β) is a protein produced in the reproductive tract of camelids (camels, llamas, and alpacas) that has been identified as the ovulation inducing factor in seminal plasma. NGF-β from seminal plasma deposited into the reproductive tract of the female camelid acts systemically to stimulate secretion of luteinizing hormone (LH) from the anterior pituitary, which in turn induces follicle maturation and ovulation. The objectives of the present study were to 1) determine if NGF-β is present in the reproductive tract of the stallion and 2) identify the specific site(s) of production. The hypotheses were that NGF-β would be present in the stallion reproductive tract and would primarily be localized in Sertoli cells of the testis and the prostate gland. Immunohistochemistry on paraffin embedded paraformaldehyde fixed tissues was performed using a rabbit polyclonal anti-NGF-β antibody on a total of six male equine reproductive tracts, including a one-day old colt, a one-year old colt and 4 adult stallion tracts. Strong immunostaining was observed in the efferent ducts of the testis and the epithelial cells of the prostate, seminal vesicles, bulbourethral glands and ampullae. Weaker NGF-β staining was noted in Leydig cells, Sertoli cells and spermatogonia within the testes and in epithelial cells of the epididymis. In conclusion, immunohistochemistry revealed that NGF-β is present in the stallion reproductive tract and the protein is primarily present in the efferent ducts of the testes and in all accessory sex glands.

Name thesis:degree_name

Master of Science (M.S.)

Level thesis:degree_level

Masters

Discipline thesis:degree_discipline

Biomedical Sciences

Grantor dc:publisher

Colorado State University. Libraries

Year dc:date.issued

2020

Authors dc:creator

• Mickelson, Alison, author
• McCue, Patrick, advisor
• Graham, James, advisor
• Magunda, Forgivemore, committee member

Statement dc:rights

• Copyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.

Language dc:language.iso

eng, English

Identifier URI

https://doi.org/10.25675/3.02502

OAI identifier oai:identifier

oai:mountainscholar.org:10217/219572