Development and application of electrochemical dithiothreitol (DTT) assay for analysis of particulate matter
type: CreativeWork
description: Particulate matter (PM) in air pollution, known to have a negative impact on biological systems, is regulated in many countries across the globe. The generation of PM from energy and mining industries is monitored in an effort to minimize its contributions to diminished human health. And although quantifying total PM generation (mass and number) and exposure can help track health risks, ultimately there exists a need to develop rapid, efficient, accurate methods for analyzing PM composition and health effects. Leading hypotheses over the last decade have theorized that PM, once absorbed into bodily tissues, generate reactive oxygen species (ROS), leading to oxidative stress, thus catalyzing cellular damage. A recently developed analytical electrochemical protocol has shown great potential for investigating the potential for PM to cause oxidative stress. The work discussed within this thesis focuses on the development of the electrochemical assay and its application to real world PM samples. Herein, the development of an electrochemical version of the well-studied dithiothreitol (DTT) absorbance spectroscopy assay is presented as a platform for the analysis of PM in air samples. Flow injection amperometry was used as the primary electrochemical method. Amperometry was performed using a CH Instruments potentiostat and commercially available DropSens modified carbon screen printed electrodes (SPE's) with a DropSens impinging jet flow cell, granting the assay flexibility to be performed in any lab with commercially available components. Previous examples used homemade components restricting accessibility to the field. The ability to use inexpensive, purchasable components eases trouble shooting, training, and allows for the potential to make the assay more mobile. The use of a flow cell also allows for the possibility for linking the assay to other analytical methods to further analyze PM which may not be reactive in the assay. Assay development focused on optimizing th…
abstract: Particulate matter (PM) in air pollution, known to have a negative impact on biological systems, is regulated in many countries across the globe. The generation of PM from energy and mining industries is monitored in an effort to minimize its contributions to diminished human health. And although quantifying total PM generation (mass and number) and exposure can help track health risks, ultimately there exists a need to develop rapid, efficient, accurate methods for analyzing PM composition and health effects. Leading hypotheses over the last decade have theorized that PM, once absorbed into bodily tissues, generate reactive oxygen species (ROS), leading to oxidative stress, thus catalyzing cellular damage. A recently developed analytical electrochemical protocol has shown great potential for investigating the potential for PM to cause oxidative stress. The work discussed within this thesis focuses on the development of the electrochemical assay and its application to real world PM samples. Herein, the development of an electrochemical version of the well-studied dithiothreitol (DTT) absorbance spectroscopy assay is presented as a platform for the analysis of PM in air samples. Flow injection amperometry was used as the primary electrochemical method. Amperometry was performed using a CH Instruments potentiostat and commercially available DropSens modified carbon screen printed electrodes (SPE's) with a DropSens impinging jet flow cell, granting the assay flexibility to be performed in any lab with commercially available components. Previous examples used homemade components restricting accessibility to the field. The ability to use inexpensive, purchasable components eases trouble shooting, training, and allows for the potential to make the assay more mobile. The use of a flow cell also allows for the possibility for linking the assay to other analytical methods to further analyze PM which may not be reactive in the assay. Assay development focused on optimizing th…
author: Turner, Laurelle Rose, author
author: Henry, Charles, advisor
author: Kipper, Matt, committee member
author: Volckens, John, committee member
language: eng
language: English
keywords: flow injection amperometry, quinone, Honduras, dithiothreitol assay
date published: 2017
date modified: 2026-07-27T19:12:54Z
provider: Colorado State University
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Colorado State University. Libraries

PropertyValue
property ID: Handle
value: https://hdl.handle.net/10217/185623

PropertyValue
property ID: DOI
value: 10.25675/3.018308